DNA replication and messenger RNA production after induction of wild-type λ bacteriophage and λ mutants
Journal of Molecular BiologyPublished 1 August 1966
Anne Joyner, Leonard N. Isaacs, Harrison Echols, William S. Sly
Citations181
SJR quartileQ1
SJR score2.21
SNIP1.13
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TL;DR
The mRNA results suggest that the pleiotropio defect in synthesis of late proteins shown by N, O, P and Q mutants results from an insufficient number of mRNA copies for the region of the A genome containing the maturation functions.
Abstract
Hybridization techniques have been used to study phage DNA synthesis and mRNA levels after induction of wild-type λ and a number of λ mutants. Mutants of λ defective in the N, O and P cistrons are blocked in DNA replication and produce a very low level of λ mRNA late in the induction period. Mutants defective in the Q cistron show normal DNA replication, but produce much lower levels of λ mRNA than a normal phage. The mRNA results suggest that the pleiotropio defect in synthesis of late proteins shown by N, O, P and Q mutants results from an insufficient number of mRNA copies for the region of the A genome containing the maturation functions. The λ T11 mutant, which has been shown previously to produce greatly elevated levels of λ nuclease, was found to be blocked in DNA replication. There does not, however, appear to be a general correlation between failure of DNA replication and the hypernuclease phenotype, because the DNA-defective. O cistron mutant has been found previously to be normal in nuclease production. The turn-on of λ-specific nucleic acid production in normal λ development after mitomycin and temperature induction has been compared. There is a 20- to 30-minute lag after addition of mitomycin before λ DNA and mRNA levels show an increase; in the case of temperature induction, the turn-on of λ nucleic acid production occurs much earlier. The effect of λ development after temperature induction on host DNA and mRNA production was also studied; no strong inhibition was found.
Keywords
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