Chapter 7 Developmental Stage-Specific Antigens During Mouse Embryogenesis
Current topics in developmental biology/Current Topics in Developmental BiologyPublished 1 January 1979
Davor Solter, Barbara B. Knowles
Citations110
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TL;DR
This chapter summarizes the results obtained so far and offers some suggestions for future experimentation on Immunological methods for the study of cell surface molecules in regulating developmental processes.
Abstract
The important role of cell surface molecules in regulating developmental processes has been assumed, but experimental evidence has been scarce. Immunological methods offer, perhaps, the best approach to the study of such molecules and this chapter summarizes the results obtained so far and offers some suggestions for future experimentation. It also describes several xenogeneic, allogeneic, and syngeneic antisera, with the common property that they all react with some or all stages of early mouse development. These antisera cross-react most notably with teratocarcinoma stem cells and sperm and with various other cell types as well. Probably all antisera described in the chapter are polyspecific and their range of specificities partially overlapped. None of these antisera react with embryos alone, with the possible exception that some are raised by syngeneic immunization with embryos. Activation of the embryonic genome occurs very early in mouse development, possibly immediately after fertilization and certainly after two divisions. It is, therefore, possible to study the activity of developmental genes in a very simple structure that is composed of only a few cells and in which gene activity directs basic events such as the separation of the embryo into its embryonic part.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described, which defines an embryonic stage-specific antigen.
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Proceedings of the Royal Society of London. Series B, Biological sciencesThe Leeuwenhoek Lecture, 1977 Mouse teratocarcinoma and mouse embryo
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Developmental BiologyDetection of sperm antigens on mouse ova and early embryos
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40 Citations1976David P. Aden, Barbara B. Knowles
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Developmental BiologyNS-6 (nervous system antigen-6): A new cell surface antigen of brain, kidney, and spermatozoa
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Developmental BiologyNS-7 (nervous system antigen-7): A cell surface antigen of mature brain, kidney, and spermatozoa shared by embryonal tissues and transformed cells
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