Autoradiographic maps of regional brain glucose consumption in resting, awake rats using [<sup>14</sup>c] 2‐deoxyglucose
The Journal of Comparative NeurologyPublished 15 January 1978
William J. Schwartz, Frank R. Sharp
Citations96
SJR quartileQ2
SJR score1.08
SNIP0.80
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The pattern of regional brain glucose consumption in the normal, resting, awake rat is presented and the anatomical identities of brain structures which on autoradiographs appear only as regional variations of optical density are presented.
Abstract
The 2-deoxy-D-[14C]-glucose (2-DG) autoradiographic method for determining regional brain glucose consumption has been applied successfully by a number of workers for mapping the alterations of brain glucose consumption which occur in association with experimental alterations of brain functional activity. This paper provides a framework for the interpretation of these and further studies by presenting: (1) the pattern of regional brain glucose consumption in the normal, resting, awake rat; (2) the anatomical identities of brain structures which on autoradiographs appear only as regional variations of optical density. For this purpose, a series of 2-DG autoradiographs of coronal brain sections from an injected animal is compared with adjacent labeled Nissl sections.
Keywords
MedicineNeuroscience
Journal of NeurochemistryTHE [<sup>14</sup>C]DEOXYGLUCOSE METHOD FOR THE MEASUREMENT OF LOCAL CEREBRAL GLUCOSE UTILIZATION: THEORY, PROCEDURE, AND NORMAL VALUES IN THE CONSCIOUS AND ANESTHETIZED ALBINO RAT<sup>1</sup>
6,025 Citations1977Louis Sokoloff, Martin Reivich +6 more
The method can be applied to most laboratory animals in the conscious state and is based on the use of 2‐deoxy‐D‐[14C]glucose as a tracer for the exchange of glucose between plasma and brain and its phosphorylation by hexokinase in the tissues.
Journal of Pharmacology and Experimental TherapeuticsDOPAMINERGIC NEURONS: EFFECT OF ANTIPSYCHOTIC DRUGS AND AMPHETAMINE ON SINGLE CELL ACTIVITY
1,284 Citations1973Benjamin S. Bunney, Judith R. Walters +2 more
Neostriatal 3,4-dihydroxyphenylacetic acid was inceased by chlorpromazine, decreaed by amphetamine and unchanged by promethazine, thus paralleling the effects of these drugs on dopaminergic unit activity, compatible with the neuronal feedback hypothesis.
The Journal of Comparative NeurologyThe organization and postnatal development of the commissural projection of the rat somatic sensory cortex
518 Citations1976S.P. Wise, Edward G. Jones
Anterograde and retrograde tracing experiments have been used to demonstrate the origin and terminal distribution of commissural fibers in the first somatosensory cortex of the rat.
Archives of NeurologyCorrelation Between Regional Cerebral Blood Flow and Oxidative Metabolism
485 Citations1976Marcus E. Raichle
In normal subjects and patients with chronic, stable diseases of brain, r CBF correlated well with rCMRO2, and in patients with acute diseases affecting the hemisphere studied, however, the correlation between rCBF and r CMRO2 was unpredictable.
ScienceMapping of Functional Neural Pathways by Autoradiographic Survey of Local Metabolic Rate with [ <sup>14</sup> C]Deoxyglucose
434 Citations1975Charles Kennedy, M. H. Des Rosiers +4 more
An enzymatic preparation from human brain converts tryptamine to tryptoline (9H-1,2,3, 4-tetrahydropyrido(3,4-b)indole) in the presence of 5-methyltetrahYDrofolic acid, which yields 1- methyltryptoline and 5-hydroxytryptoline, respectively.
ScienceSuprachiasmatic Nucleus: Use of <sup>14</sup> C-Labeled Deoxyglucose Uptake as a Functional Marker
354 Citations1977William J. Schwartz, Harold Gainer
The results show that glucose consumption of the SCN, in contrast to other brain structures, is a function of both the time of day and environmental lighting conditions, and indicate that the DG technique may provide a novel approach for the study of the central neural mechanisms underlying circadian rhythm regulation.
Proceedings of the National Academy of SciencesMetabolic mapping of the primary visual system of the monkey by means of the autoradiographic [14C]deoxyglucose technique.
308 Citations1976Charles Kennedy, M.H. Des Rosiers +5 more
An autoradiographic technique that employs 2-[14-C]deoxyglucose to measure the local rates of glucose utilization within the brain has been applied to the binocular visual system of the Macaque monkey.
Archives of NeurologyMetabolic Anatomy of Focal Motor Seizures
198 Citations1976Robert C. Collins, C. Kennedy +2 more
The anatomic dimensions of the metabolic response in the focus as well as the spread of increased activity through the brain were studied by autoradiography following intravenous injection of carbon 14-labeled 2-deoxyglucose.
Journal of NeurochemistryENZYMIC AND CEREBRAL METABOLIC EFFECTS OF 2‐DEOXY‐<scp>d</scp>‐GLUCOSE
164 Citations1973Roger W. Horton, Brian S. Meldrum +1 more
Analysis of the fate of d‐[U‐14C] glucose given subcutaneously 5 min before death showed that glucose uptake was reduced, however brain glucose concentration rose three to five‐fold 20–30 min after 2‐deoxy‐d‐glucose, and the majority of glucose entering the brain after 10 min of 2‐ deoxy‐ d‐glUCose treatment was recovered as glucose.
Journal of NeurochemistrySPECIFICITY AND KINETIC PROPERTIES OF MONOSACCHARIDE UPTAKE INTO GUINEA PIG CEREBRAL CORTEX IN VITRO
154 Citations1971H. S. Bachelard
Evidence on competition with glucose for the transport process has been used to derive information on the substrate specificity of sugar uptake to the brain and the kinetic properties of the uptake of 2‐deoxygIucose indicate that the transport is a facilitated process rather than diffusion.
Brain ResearchRelative cerebral glucose uptake of neuronal perikarya and neuropil determined with 2-deoxyglucose in resting and swimming rat
122 Citations1976Frank R. Sharp
Since gray matter is composed predominantly of neuropil, the present results indicate that the bulk of the total brain gray matter glucose consumption is accounted for by Neuropil in both the resting and swimming states.
Journal of Biological ChemistryThe Kinetics of Glycogen Phosphorylases from Brain and Muscle
113 Citations1967Oliver H. Lowry, Demoy W. Schulz +1 more
Under the experimental conditions there was found little evidence of interaction between phosphate and adenylate sites, although from a few experiments under different conditions and from the work of others, some interaction is possible.
Journal of NeurochemistryHEXOSE TRANSLOCATION ACROSS THE BLOOD‐BRAIN INTERFACE: CONFIGURATIONAL ASPECTS<sup>1</sup>
85 Citations1968T. George Bidder
The translocation of intravenously administered tritiated 3‐O‐methyl‐d‐glucopyranose into brain has been studied in an intact nephrectomized rat preparation by infusing a series of hexoses into one internal carotid artery.
NatureLesions of ascending dopaminergic pathways decrease forebrain glucose uptake
68 Citations1976William J. Schwartz, Frank R. Sharp +2 more
It is found that destruction of dopaminergic fibres results in decreasedForebrain glucose uptake following various lesions of ascending catecholaminergic pathways.
Brain ResearchSpontaneous firing rate of neurones in the prefrontal cortex of the rat: evidence for a dopaminergic inhibition
57 Citations1976Francisco Mora, K. F. Sweeney +2 more
The first electrophysiological and pharmacological investigation into the possible role of this dopaminergic input to the prefrontal cortex is described and results are found in both groups of rats independent of the anaesthetic used.
Experimental Brain ResearchInput from trigeminal cutaneous afferents to neurones of the inferior olive in rats
45 Citations1976Justin R. Cook, M. Wiesendanger
It is concluded that neurones of the inferior olive receive a substantial input from trigeminal afferents and are capable of transmitting precise somatotopical information to the cerebellum.
Brain ResearchRotation induced increases of glucose uptake in rat vestibular nuclei and vestibulocerebellum
44 Citations1976Frank R. Sharp
A new technique for the autoradiographic measurement of regional cerebral glucose consumption using tracer amounts of radioactive 2-deoxyglucose (2-DG) was employed to study the effects of vestibular inputs on local cerebral glucose metabolism.
Experimental NeurologyEffect of substantia nigra stimulation on spontaneous unit activity in the caudate nucleus
5 Citations1975Nachum Dafny
It is suggested that unit that exhibit spontaneous activity in the caudate nucleus and which respond to stimulation of the substantia nigra have polysynaptic connections, and those units which do not discharge spontaneously but respond to stimulated nucleus by driven action potentials have monosynaptic connetion to the caUDate nucleus.