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Comparison of DNA Amplification Methods for the Detection of Chlamydia trachomatis in First-Void Urine From Asymptomatic Military Recruits

Sexually Transmitted DiseasesPublished 1 March 1996
Angelika Stary, Susan Tomazic-Allen, BELINDA CHOUEIRI, John D. Burczak, Kurt Steyrer, Helen Lee
Citations68
SJR quartileQ1
SJR score0.97
SNIP0.89

TL;DR

It was found that freezing and thawing the specimens before testing enhanced the performance of PCR, and the LCx assay performed better than the Amplicor assay and appears reliable for urine testing.

Abstract

The LCx assay performed better than the Amplicor assay and appears reliable for urine testing. The low sensitivity of the Amplicor assay requires further evaluation of possible inhibitors of PCR in fresh specimens. It was found that freezing and thawing the specimens before testing enhanced the performance of PCR.

Keywords

Immunology and MicrobiologyMedicine