Isolation of Canine Cardiac Sarcolemmal Vesicles
Published 1 January 1984
Larry R. Jones, Henry R. Besch
Citations56
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TL;DR
It seems highly advantageous to isolate sarcolemmal membrane fragments from the heart in the form of tightly sealed vesicles so that not only enzymic activity but also active and passive transport of ions can be measured in the same membrane preparation.
Abstract
Isolation and characterization of sarcolemmal membrane fragments from the heart has been a goal of several research laboratories for some time (Wollenberger and Will, 1978). This is because of the fundamental importance of the sarcolemma in maintaining the electrophysiological and contractile properties of the heart by regulating transmembrane fluxes of the inotropically active cations Na+, K+, and Ca2+ between intracellular and extracellular compartments. Both purity and morphological form of the isolated membranes are equally important considerations in any isolation scheme for cardiac sarcolemma (Besch et al., 1976). In analogy to results first observed with cardiac sarcoplasmic reticulum membrane preparations, it seems highly advantageous to isolate sarcolemmal membrane fragments from the heart in the form of tightly sealed vesicles so that not only enzymic activity but also active and passive transport of ions can be measured in the same membrane preparation.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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383 Citations1979Larry R. Jones, Henry R. Besch +3 more
The results suggest that sarcolemma may contain an integral enzyme complex, not present in sarcoplasmic reticulum, that contains beta-adrenergic receptors, adenylate cyclase, cyclic AMP-dependentprotein kinase, and several substrates of the protein kinase.
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279 Citations1980Pico Caroni, Ernesto Carafoli
In the present work, an ATP-dependent Ca2+ transport system which can be conclusively ascribed to heart sarcolemma is described and it is shown that a heart vesicular preparation particularly enriched in sar colemma markers accumulates Ca2- in the presence of ATP.
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238 Citations1980Larry R. Jones, Stephen W. Maddock +1 more
The results suggest that alamethicin disrupts membrane barriers of sarcolemmal vesicles, giving substrates and activators access to enzymic sites in the interior of the vesicle, while preserving functional coupling of enzymes to their effectors.
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75 Citations1981Larry R. Jones, Stephen W. Maddock +1 more
The results suggest that cardiac sarcolemma contains an intrinsic type II cyclic AMP-dependent protein kinase activity that is not present in sarcoplasmic reticulum.
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75 Citations1978A Wollenberger, Horst Will
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46 Citations1978Larry R. Jones, Henry R. Besch +1 more
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