Emerging techniques: Cryo-electron microscopy of vitrified biological specimens
Trends in Biochemical SciencesPublished 1 April 1985
Jacques Dubochet, M. Adrian, Jean Lepault, Alasdair W. McDowall
Citations106
SJR quartileQ1
SJR score4.16
SNIP2.34
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Abstract
Rapid freezing has long promised the most faithful structural presentation of biological material for electron microscopy. With the recent emergence of methods for vitrifying specimens, this promise seems about to be fulfilled.
Keywords
Materials ScienceBiochemistry, Genetics and Molecular BiologyPhysics and Astronomy
NatureCryo-electron microscopy of viruses
1,321 Citations1984Marc Adrian, Jacques Dubochet +2 more
Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method.
Journal of MicroscopyElectron microscopy of frozen water and aqueous solutions
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High-resolution electron diffraction patterns have been obtained from frozen, hydrated catalase crystals to demonstrate the feasibility of using a frozen specimen hydration technique.
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299 Citations1983Alasdair W. McDowall, Jen-Wei Chang +4 more
The preparation and high resolution observation of frozen hydrated thin sections has been studied by transmission electron microscopy on model systems, including pure water, protein solutions, catalase crystals, myelin sheath and various tissues.
Journal of BacteriologyElectron microscopy of frozen-hydrated bacteria
252 Citations1983Jacques Dubochet, Alasdair W. McDowall +3 more
It is confirmed that "mesosomes," hitherto quite often considered to be essential organelles in all procaryotes, are artifacts, and appear in large numbers during osmium fixation.
Journal of MicroscopyElectron microscopy of frozen biological suspensions
236 Citations1983Jean Lepault, Frank P. Booy +1 more
It is shown that optimum structural preservation is obtained from a thin, quench‐frozen film with the bulk aqueous medium in the vitreous state, and frozen‐hydrated, freeze-dried or sugar embedded crystals can withstand a three‐ to four‐fold increase in electron exposure for the same damage when compared with similar sugar‐embedded or freeze‐dried samples at room temperature.
UltramicroscopyMolecular structure determination of crystalline specimens in frozen aqueous solutions
80 Citations1984Ronald A. Milligan, Alain Brisson +1 more
The freezing method appears to provide the most complete and accurate descriptions of eukaryotic ribosome crystals, bladder membranes and gap junctions preserved in frozen aqueous solutions under conditions where either amorphous or crystalline ice is formed.
The EMBO JournalProjected structure of unstained, frozen‐hydrated T‐layer of Bacillus brevis.
77 Citations1984Jean Lepault, Tony Pitt
It is shown that the contrast of T‐layer embedded in ice can be approximated to pure phase contrast.
Philosophical magazineOn the heat capacity, entropy and ‘glass transition’ of vitreous ice
68 Citations1977G. P. Johari
Journal of Molecular BiologyCryo-electron microscopy of vitrified insect flight muscle
62 Citations1984Alasdair W. McDowall, W. Hofmann +3 more
Frozen hydrated sections of vitrified unstained insect flight muscle in the rigor and relaxed states were studied by cryo-electron microscopy and it seems that the preserved structure of the relaxed structure seems better than in freeze-etched replicas.