Micro estimation of RNA by the cupric ion catalyzed orcinol reaction
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TL;DR
If such an assay is carried out under the optimal conditions described here it would appear to be the most sensitive, accurate, and rapid method for routine RNA estimation currently available.
Abstract
A method for estimation of purified RNA using cupric ion catalyzed orcinol has been studied and improved. If such an assay is carried out under the optimal conditions described here it would appear to be the most sensitive, accurate, and rapid method for routine RNA estimation currently available. The improved conditions include a combination of several factors such as replacement of the usual FeCl3 by a copper salt, increasing the concentration of orcinol in the reaction mixture, and increasing the heating time from the commonly used 20 minutes to 35 minutes.