Scintillation proximity assay
NaturePublished 1 September 1989
Nigel Bosworth, Pat Towers
Citations220
SJR quartileQ1
SJR score18.29
SNIP10.16
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TL;DR
Fluomicrospheres coated with antibodies or receptors eliminate the need to separate bound from free ligand in both radioimmunoassays and ligand-binding assays.
Abstract
Fluomicrospheres coated with antibodies or receptors eliminate the need to separate bound from free ligand in both radioimmunoassays and ligand-binding assays.
Keywords
Materials ScienceMedicine
Molecular ImmunologyScintillation Proximity Assay (SPA)—A new method of immunoassay
107 Citations1979Hiram E. Hart, Elaine B. Greenwald
Scintillation Proximity Assay was used to measure concentrations of human albumin (HA) and rabbit anti-human album in rabbits and found that concentrations of less than 1 ng/ml of HA were easily and reproducibly detected.
Analytical BiochemistryScintillation proximity assay: A sensitive and continuous isotopic method for monitoring ligand/receptor and antigen/antibody interactions
93 Citations1987Sidney Udenfriend, Louise Gerber +1 more
The principles of SPA, utilizing 125I-labeled molecules, are discussed and some applications to immunology, receptor binding, and measurement of potential across membranes are presented.
Proceedings of the National Academy of SciencesScintillation proximity radioimmunoassay utilizing 125I-labeled ligands.
80 Citations1985Sidney Udenfriend, Louise Gerber +2 more
Application of scintillation proximity immunoassay to tissue enkephalins, serum thyroxin, and urinary morphine is described and applications of the principle to study the kinetics of interaction between receptors and ligands are discussed.
Analytical BiochemistryA novel method for the detection of receptors and membrane proteins by scintillation proximity radioassay
39 Citations1987Nathan Nelson
A rapid and convenient binding assay for receptors and membrane proteins has been developed based on the binding of 125I-labeled ligands to membrane proteins adsorbed to polyvinyltoluene plastic scintillation microspheres that can be assayed by measuring competition with the labeled toxin.