Polyamine activation of staphylococcal nuclease
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisPublished 1 April 1975
Joseph J. Frank, Inez A. Hawk, Carl C. Levy
Citations25
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TL;DR
If low concentrations of calcium are added to the polyamine-activated enzyme, up to double the expected activity is observed and the polyamines have been shown to prevent inhibition of the nuclease by the ordered polynucleotides.
Abstract
The polyamines have been used to activate staphylococcal nuclease for degradation of both RNA and DNA in the absence of calcium ions. If low concentrations of calcium are added to the polyamine-activated enzyme, up to double the expected activity (based on additive values) is observed. The polyamines have also been shown to prevent inhibition of the nuclease by the ordered polynucleotides.
Keywords
Biochemistry, Genetics and Molecular BiologyEnvironmental Science
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