Synthesis and assembly of hepatitis B virus surface antigen particles in yeast
NaturePublished 1 July 1982
Pablo Valenzuela, Angélica Medina, William J. Rutter, Gustav Ammerer, Benjamin D. Hall
Citations875
SJR quartileQ1
SJR score18.29
SNIP10.16
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The surface antigen of hepatitis B virus has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences.
Abstract
The surface antigens of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5'-flanking region of yeast alcohol dehydrogenase I as a promotor to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.
Keywords
Immunology and MicrobiologyBiochemistry, Genetics and Molecular Biology
NatureCleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
251,640 Citations1970Ulrich K. Laemmli
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Nucleic Acids ResearchA rapid alkaline extraction procedure for screening recombinant plasmid DNA
14,877 Citations1979H.C. Birnboim, Janine Doly
The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
European Journal of BiochemistryA Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
8,651 Citations1974William M. Bonner, Ronald A. Laskey
A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
CellIdentification of 23 complementation groups required for post-translational events in the yeast secretory pathway
1,815 Citations1980Peter Novick, C Field +1 more
Electron microscopy of sec mutant cells reveals the temperature-dependent accumulation of membrane-enclosed secretory organelles, and it is suggested that these structures represent intermediates in a pathway in which secretion and plasma membrane assembly are colinear.
NatureTransformation of yeast by a replicating hybrid plasmid
1,335 Citations1978Jean D. Beggs
These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency and contained multiple plasmid copies which were recovered by transformation in E. coli.
NatureNucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli
987 Citations1979Francis Galibert, Elisabeth Mandart +3 more
The complete nucleotide sequence of hepatitis B virus genome (subtype ayw) cloned in Escherichia coli has been determined using the Maxam and Gilbert method and the dideoxynucleotide method.
NatureHepatitis B virus genes and their expression in E. coli
582 Citations1979Mark Pasek, Tadaatsu Goto +6 more
A composite DNA sequence of regions of hepatitis B virus, determined from a series of recombinant plasmids, reveals the genes for the surface antigen and the core antigen of the virus.
GeneSequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene
509 Citations1980Gary Tschumper, John Carbon
The DNA sequence of a 1.45 kb EcoRI fragment from the yeast (Saccharomyces cerevisiae) TRP1 region has been determined and theTRP1 gene has been located on the fragment by analysis of potential initiation and termination codons in the DNA sequence.
ScienceHuman Growth Hormone: Complementary DNA Cloning and Expression in Bacteria
405 Citations1979Joseph Martial, Robert A. Hallewell +2 more
The data reported predict the previously unknown sequence of the signal peptide of human growth hormone and strengthens the hypothesis that these genes evolved by gene duplication from a common ancestral sequence.
PubMedEstablishment of a continuously growing cell line from primary carcinoma of the liver.
338 Citations1976J. J. Alexander, E. Bey +2 more
A continuously growing cell line has been established from autopsy material taken from a primary liver carcinoma, a cancer which has a high incidence in Southern Africa, and adaptation to in vitro conditions was completed after 18 months.
NatureExpression of a human gene for interferon in yeast
328 Citations1981Ronald A. Hitzeman, Frank E. Hagie +4 more
A DNA sequence coding for mature human leukocyte interferon D (LeIF-D) was linked with DNA fragments of the 5′-flanking sequences of the Saccharomyces cerevisiae (yeast) alcohol dehydrogenase I gene in a plasmid capable of autonomous replication and selection in both yeast and Escherichia coli.
Proceedings of the National Academy of SciencesGenome of hepatitis B virus: restriction enzyme cleavage and structure of DNA extracted from Dane particles.
322 Citations1975Jesse Summers, Anne R. O’Connell +1 more
D Dane particle DNA is proposed to be a double-stranded circular DNA approximately 3600 nucleotides in length containing a single-stranding gap of 600-2100 nucleosidetriphosphate and the endogenous DNA polymerase reaction appears to repair this single-Stranded gap.
British Journal of CancerHepatitis B surface antigen produced by a human hepatoma cell line
314 Citations1976G M Macnab, J. J. Alexander +3 more
NatureNucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen
303 Citations1979Pablo Valenzuela, Patrick W. Gray +4 more
The amino acid sequence deduced from the DNA indicates that the surface antigen is a protein consisting of 226 amino acids and with a molecular weight of 25,398, and the portion of the gene coding for this protein apparently contains no intervening sequences.
NatureExpression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322
244 Citations1979C. J. Burrell, Patricia Mackay +3 more
Fragments of hepatitis B virus DNA isolated from Dane particles have been inserted into the Escherichia coli plasmid pBR322 and cloned and synthesised antigenic material that reacts specifically with antisera to hepatitis B viral antigens.
Annual Review of BiochemistryMolecular Genetics of Yeast
222 Citations1980T D Petes
The present work presents a meta-analysis of yeast chromosomol genes that highlights the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals.
NatureElectron microscopy of hepatitis B core antigen synthesized in E. coli
157 Citations1982B J Cohen, J. E. Richmond
It is reported that the E. coli HBcAg preparation contains Small round particles similar in appearance to the viral cores previously seen in material obtained from HBV-infected humans5–7 and chimpanzees8.
Journal of VirologyDNA Polymerase in the Core of the Human Hepatitis B Virus Candidate
154 Citations1974William S. Robinson, Richard L. Greenman
The DNA polymerase and the radioactive DNA reaction product were used as markers for core in immunoprecipitation tests for anticore and 8 of 11 human sera with anti-HB(s) activity and all of 10 sera from chronic HBAg carriers were found to contain anti- HB(c) activity.
Journal of Biological ChemistryIsolation and characterization of the major protein and glycoprotein of hepatitis B surface antigen.
146 Citations1981D L Peterson
Hepatitis B surface antigen has been purified and its major protein (p-25) and glycoprotein (gp-30) isolated and it was demonstrated that p-25 and gp-30 have identical protein structure, differing only by the presence of carbohydrate in gp- 30.
NatureCloning and endonuclease mapping of the hepatitis B viral genome
119 Citations1979John J. Sninsky, Aleem Siddiqui +2 more
The cloning of double-stranded HBV DNA in Escherichia coli K12 is reported, using the unique EcoRI cleavage site on the viral genome to introduce the entire HBVDNA molecule into an EcoRI Cleavage site within the chloroamphenicol resistance gene of the pACYC184 plasmid vector1.
Journal of VirologyAustralia Antigen: Large-Scale Purification from Human Serum and Biochemical Studies of Its Proteins
117 Citations1971John L. Gerin, P. V. Holland +1 more
A three-step procedure involving isopycnic banding of the particle in CsCl density gradients and rate-zonal centrifugation on sucrose gradients resulted in a highly purified Au preparation which was used for biochemical studies of Au proteins and as immunizing antigen for the production of reagent antiserum in animals.
Journal of VirologyBiophysical Properties of Australia Antigen
116 Citations1969John L. Gerin, R H Purcell +3 more
Biophysical studies with Australia complement-fixing antigen showed it to be a particle with a buoyant density of 1.20 g/cm(3) in CsCl, a sedimentation coefficient of 110, and an average diameter of 25 nm.
Journal of General VirologyCellular and Humoral Immunity in Guinea Pigs to Two Major Polypeptides Derived from Hepatitis B Surface Antigen
57 Citations1978G. A. Cabral, Francine Marciano‐Cabral +5 more
The humoral immune response to either of the polypeptides, as measured by radioimmunoassay, was substantially lower than that observed in animals immunized with intact particles, suggesting that the macrophage migration assay is a sensitive indicator of the immunogenicity of the smaller mol.
Proceedings of the National Academy of SciencesProduction of immunologically active surface antigens of hepatitis B virus by Escherichia coli.
54 Citations1981Patricia Mackay, M. Pasek +8 more
The results emphasize the value of an in vivo assay for the presence of antigens in crude cell extracts and illustrate the feasibility of this type of screening with laboratory animals.
Journal of Medical VirologyThe proteins of hepatitis B dane particle cores
45 Citations1977Jerome F. Hruska, William S. Robinson
Dane particles and Dane particle cores purified by equilibrium CsCl density gradient centrifugation and HBcAg particles from HBV infected chimpanzee liver purified in the same way were analyzed and both kinds of particles were found to consistently contain 3 Coomassie blue staining peptides with approximate molecular weights of 19,000, 70,000 and 80,000 daltons.
Journal of VirologyProteins of hepatitis B surface antigen: amino acid compositions of the major polypeptides
29 Citations1977J W Shih, John L. Gerin
A high degree of compositional relatedness is indicated between the P-1 and P-2 polypeptides for each of the two preparations of hepatitis B surface antigen/adw.
Journal of Toxicology and Environmental HealthRole of hepatitis b virus in primary liver cancer
9 Citations1979Arie J. Zuckerman
It is likely that primary liver cancer is the cumulative result of several cofactors including infection with hepatitis B virus; genetic, immunologic, hormonal, and nutritional factors; and environmental factors including mycotoxins and chemical carcinogens.