A method for studying collagen biosynthesis in human skin biopsies in vitro
Biochimica et Biophysica Acta (BBA) - General SubjectsPublished 1 March 1970
Jouni Uitto
Citations82
SJR quartileQ2
SJR score0.77
SNIP0.66
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TL;DR
When skin biopsy specimens from living human subjects of various ages were used for incubation, younger subjects demonstrated a relatively high rate of collagen synthesis and a decrease with advancing age was observed.
Abstract
Biosynthesis of collagen was studied by incubating slices of human skin in a medium containing [14C]proline. The presence of hydroxy[14C]proline in nondialysable protein of the skin was taken as a measure of collagen formation. The synthesis of radioactive hydroxyproline was studied under various experimental conditions, and an incubation medium (20 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid buffer (pH 7.4), so mM glucose, 0.05 mg/ml ampicillin, I μC [14C]proline, all in phosphate-free Krebs-Ringer solution) for an optimal rate of collagen synthesis is presented. The separate components of the incubation medium had no inhibitory effect on the hydroxylation of [14C]proline when partially purified preparations of protocollagen proline hydroxylase were incubated with radioactive protocollagen substrate. When skin biopsy specimens from living human subjects of various ages were used for incubation, younger subjects demonstrated a relatively high rate of collagen synthesis and a decrease with advancing age was observed.
Keywords
Biochemistry, Genetics and Molecular Biology
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