The Development and Application of a Direct Radioimmunoassay for Plasma Aldosterone Using<sup>125</sup>I-Labeled Ligand—Comparison of Three Methods*†
The Journal of Clinical Endocrinology & MetabolismPublished 1 January 1978
Emad A. S. Al‐Dujaili, C.R.W. Edwards
Citations83
SJR quartileQ1
SJR score2.18
SNIP1.77
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TL;DR
A direct, rapid, sensitive, and highly specific radioimmunoassay for plasma aldosterone has been developed using a Aldosterone conjugated to [125I]histamine as ligand and a highly specific ald testosterone antiserum.
Abstract
A direct, rapid, sensitive, and highly specific radioimmunoassay for plasma aldosterone has been developed using aldosterone conjugated to [125I]histamine as ligand and a highly specific aldosterone antiserum. This assay permits the direct measurement of plasma aldosterone without preliminary extraction or purification steps and hence allows a single technician to assay 500 samples in a week. An excellent correlation was obtained between the results of the direct assay and the levels measured after paper chromatography (n = 43, r = 0.99, P less than 0.001) or after extraction (n = 43, r = 0.99, P less than 0.001). The coefficients of variation for intra-assay and inter-assay determinations of samples from a normal plasma pool were 7.6% and 9.3%, respectively.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
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