Bradyrhizobium Japonicum Genes Involved in Soybean Root-Nodule Development
Published 1 January 1986
J. W. Lamb, Brigitte Regensburger, H. J. Fisher, Michael Göttfert, Linda Meyer, Sabine Ebeling
Citations6
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TL;DR
In this report, some promising mutants of B. japonicum which are defective in different steps during bacteroid and root nodule development are described and a progress report on the isolation and characterization of the B. Japonicum nod genes is presented.
Abstract
Bradyrhizobium japonicum is a member of the slow-growing genus of the rhizobia. It is able to form determinate, N2 fixing root nodules on soybean (Glycine max L. Merr.). Research in our laboratory is concerned with the structural and functional analysis of bacterial genes involved in nodulation and symbiotic nitrogen fixation. We have chosen B. japonicum strain 110 for this study, as this strain is able to derepress nitrogenase activity under free-living, microaerobic growth conditions (ex planta). In this report we describe some promising mutants of B. japonicum which are defective in different steps during bacteroid and root nodule development. Furthermore, we present a progress report on the isolation and characterization of B. japonicum nod genes.
Keywords
Agricultural and Biological Sciences
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372 Citations1985Bernard J. Carroll, D. L. McNeil +1 more
Results indicate that nts382 is affected in a nodule-development regulatory gene and not in a gene related to nitrate assimilation, and suggest that nitrate stimulated growth in both the wild type and nt382, and these lines had similar nitrate reductase activity.
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In a split-root system of soybeans (Glycine max L. Merr), inoculation of one half-side suppressed subsequent development of nodules on the opposite side, and nodule suppression was significantly more pronounced in the shaded treatments.
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A protein comparison of the nodF protein showed it to be similar to the acyl‐carrier protein from Escherichia coli and barley, especially around the pantothenate‐binding region and on this basis it is thought that this protein may be involved in an acyl transfer reaction.
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The rapid regulatory response may be an important factor contributing to the clustering of nodules in the crown region of soybean roots in field-grown plants and the sparse nodulation commonly observed in younger regions of the root.
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113 Citations1986Peter R. Schofield, John M. Watson
DNA sequencing technique has been used to determine the precise locations of Tn5 mutations within the nod genes and the phenotypes of the corresponding mutants correlate with their mapped locations, suggesting that translational coupling may ensure the synthesis of equimolar amounts of these gene products.
The EMBO JournalThe pleiotropic nature of symbiotic regulatory mutants: Bradyrhizobium japonicum nifA gene is involved in control of nif gene expression and formation of determinate symbiosis
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Results show that nifA not only controls nitrogenase genes but also one or more genes involved in the establishment of a determinate, nitrogen‐fixing root nodule symbiosis in a slow‐growing soybean symbiont.
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Nucleotide sequence analysis of three copies of RS alpha revealed many characteristics of procaryotic insertion sequence elements: potential inverted repeats at their ends, potential target site duplication, and large open reading frames, despite this, their genomic positions appear to be stable.
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90 Citations1986Kieran F. Scott
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Several classes of deletion mutants were found which, as judged by their physical DNA structure and their phenotypes, allowed the following most important conclusions: (i) deletions lacking both the nifDK and nifH regions indicate linkage between the two operons whereby at least 15 kb of DNA separate them.
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Molecular and General Genetics MGGIn Bradyrhizobium japonicum the common nodulation genes, nodABC, are linked to nifA and fixA
39 Citations1986J. W. Lamb, Hauke Hennecke
Using cloned Rhizobium phaseoli nodulation (nod) genes as hybridization probes homologous restriction fragments were detected in the genome of the slow-growing soybean symbiont, BradyrhizOBium japonicum strain 110, and mutants carrying this insertion were completely nodulation deficient.
Current plant science and biotechnology in agricultureOrganization and Regulation of Symbiotic Nitrogen Fixation Genes from Bradyrhizobium Japonicum
18 Citations1985Hauke Hennecke, Ariel Álvarez-Morales +12 more
Recent data on the analysis of bacterial genes required for the formation of a nitrogen fixing soybean root nodule are summarized, with particular emphasis on nif and fix genes.