Self-resistance of a Streptomyces which produces istamycins.
The Journal of AntibioticsPublished 1 January 1981Open access
Hirokazu Yamamoto, Kunimoto Hotta, Yoshirō Okami, HAMAO UMEZAWA
Citations27
SJR quartileQ3
SJR score0.48
SNIP0.83
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TL;DR
Using reciprocally reconstituted systems consisting of S-150 fractions and ribosomes from strain SS-939 and those from Streptomyces griseus ISP5236 (istamycin-sensitive strain), ribosome were found to be resistant to istamycins.
Abstract
Streptomyces tenjimariensis SS-939, a producer of istamycins, is highly resistant to its own antibiotics and grows in Tryptic Soy Broth containing istamycin A or B at 3,000 microgram/ml. No istamycin-inactivating enzyme was detected in extracts of strain SS-939. Polyphenylalanine synthesis in an in vitro system, consisting of the S-150 fraction and ribosomes prepared from strain SS-939, was not inhibited by 200 microgram/ml of istamycins. Using reciprocally reconstituted systems consisting of S-150 fractions and ribosomes from strain SS-939 and those from Streptomyces griseus ISP5236 (istamycin-sensitive strain), ribosomes of strain SS-939 were found to be resistant to istamycins. Thus, ribosomes have the main role in the self-resistance mechanism of S. tenjimariensis SS-939.
Keywords
ChemistryMedicineBiochemistry, Genetics and Molecular Biology
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