Clathrin, Adaptors, and Sorting
Annual Review of Cell BiologyPublished 1 November 1990
Barbara M. F. Pearse, Margaret S. Robinson
Citations701
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TL;DR
Clathrin-Mediated Intracellular Sorting in Yeast Cells Lacking Clathrin and Interaction of Receptors with Adaptors.
Abstract
CONTENTS INTRODUCTION 15 1 COAT COMPONENTS 153 Clathrin Triskelions and Adaptors ....... 153 Function and Distribution of Adaptors 156 COATED PITS AS MOLECULAR FILTERS ......... ... .. 159 Clathrin-Mediated Endocytosis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 Clathrin-Mediated Intracellular Sorting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 62 Defective Sorting in Yeast Cells Lacking Clathrin ... 165 Interaction of Receptors with Adaptors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166 CONCLUSIONS AND PROBLEMS 167
Keywords
Biochemistry, Genetics and Molecular Biology
CellA C-terminal signal prevents secretion of luminal ER proteins
2,214 Citations1987Sean Munro, Hugh R.B. Pelham
It is proposed that the KDEL sequence marks proteins that are to be retained in the ER and discuss possible retention mechanisms.
NatureCoated pits, coated vesicles, and receptor-mediated endocytosis
2,072 Citations1979Joseph L. Goldstein, Richard G.W. Anderson +1 more
It is now recognised that receptor-mediated endocytosis has a fundamental role in the growth, nutrition and differentiation of animal cells.
Annual Review of BiochemistryGROWTH FACTOR RECEPTOR TYROSINE KINASES
1,831 Citations1988Yosef Yarden, A. Ullrich
Annual Review of Cell BiologyReceptor-Mediated Endocytosis: Concepts Emerging from the LDL Receptor System
1,604 Citations1985Joseph L. Goldstein, Michael S. Brown +3 more
This data indicates that intracellular routes in the response of the immune system to EMT are regulated by Tournaisian reprograming, a type of ‘spatially aggregating’ behaviour.
CellThe human LDL receptor: A cysteine-rich protein with multiple Alu sequences in its mRNA
1,406 Citations1984Tokuo Yamamoto, Christopher G. Davis +5 more
Transfection of simian COS cells with the human LDL receptor cDNA linked to the SV40 early promoter resulted in expression of functional cell surface receptors.
Annual Review of BiochemistryBIOSYNTHETIC PROTEIN TRANSPORT AND SORTING BY THE ENDOPLASMIC RETICULUM AND GOLGI
992 Citations1987Suzanne R. Pfeffer, James E. Rothman
Annual Review of BiochemistryLYSOSOMAL ENZYMES AND THEIR RECEPTORS
919 Citations1986Kurt Von Figura, Andrej Hasilík
CellRole of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts
694 Citations1977Richard G.W. Anderson, Michael S. Brown +1 more
The current data indicate that the coated regions of plasma membrane are specialized structures of rapid turnover that function to carry receptor-bound LDL, and perhaps other receptor- bound molecules, into the cell.
Journal of Molecular BiologyCoated vesicles from pig brain: Purification and biochemical characterization
558 Citations1975Barbara M. F. Pearse
The most striking observation about these vesicles, reported here, is that they contain essentially just one protein species, having an apparent molecular weight on sodium dodecyl sulphate gels of about 180,000.
NatureThe receptor for transepithelial transport of IgA and IgM contains multiple immunoglobulin-like domains
506 Citations1984Keith E. Mostov, Martin Friedlander +1 more
The cDNA for the receptor that mediates the endocytosis and transcellular transport of IgA and IgM across many glandular epithelia into external secretions is cloned and sequenced.
The Journal of Cell BiologyDistinct endocytotic pathways in epidermal growth factor-stimulated human carcinoma A431 cells [published erratum appears in J Cell Biol 1990 Mar;110(3):859]
454 Citations1989Michele West
It is shown that in the absence of extracellular Na+ or in the presence of amiloride the stimulation of pinocytosis by EGF is substantially reduced, and EGF induces a distinct amILoride-sensitive endocytotic pathway on A431 cells.
Annual Review of BiochemistryMembrane Recycling by Coated Vesicles
448 Citations1981Barbara M. F. Pearse, Mark S. Bretscher
The Journal of Cell BiologyAn enzyme that removes clathrin coats: purification of an uncoating ATPase.
442 Citations1984David M. Schlossman, Sandra L. Schmid +2 more
Uncoating ATPase, an abundant 70,000-mol-wt polypeptide mediating the ATP-dependent dissociation ofClathrin from coated vesicles and empty clathrin cages, has been purified to virtual homogeneity from calf brain cytosol.
NatureAssembly units of clathrin coats
420 Citations1981Ernst Ungewickell, Daniel Branton
It is shown that purified clathrin coats dissociate reversibly into triskelions, structures composed of three usually bent, rather flexible legs radiating from a centre, and the molecular weight of these tr iskelions is determined and it is concluded that they contain trimers of clathin together with about three light molecular weight polypeptide chains.
ScienceIntracellular Targeting and Structural Conservation of a Prohormone-Processing Endoprotease
396 Citations1989Robert S. Fuller, Anthony J. Brake +1 more
Analysis of mutant kex2 genes truncated at their 3' end indicates that carboxyl terminal domains of the enzyme are required for its proper localization within the cell.
ScienceA Single Receptor Binds Both Insulin-Like Growth Factor II and Mannose-6-Phosphate
371 Citations1988Richard G. MacDonald, Suzanne R. Pfeffer +8 more
Results indicate that the type II IGF receptor contains cooperative, high-affinity binding sites for both IGF-II and Man-6-P-containing proteins.
The EMBO JournalReceptors compete for adaptors found in plasma membrane coated pits.
363 Citations1988Barbara M. F. Pearse
The results imply that the HA‐II complex has a recognition site for a common signal, probably involving a tyrosine residue, carried by the LDL receptor and competing receptors also found in plasma membrane coated pits.
Journal of Molecular BiologyAdsorptive endocytosis of Semliki Forest virus
347 Citations1980Mark Marsh, Ari Helenius
The Journal of Cell BiologyAcidification of the cytosol inhibits endocytosis from coated pits.
342 Citations1987Kirsten Sandvig, Sjur Olsnes +2 more
The data indicate that the reason for the reduced endocytic uptake of transferrin at internal pH less than 6.5 is an inhibition of the pinching off of coated vesicles, and are consistent with the possibility that an alternativeendocytic pathway involving smooth (uncoated) pits exists.
CellThe human transferrin receptor gene: genomic organization, and the complete primary structure of the receptor deduced from a cDNA sequence
339 Citations1984Alan McClelland, Lukas C. Kühn +1 more
The deduced amino acid sequence of the receptor shows that it does not contain an N-terminal hydrophobic signal peptide, which leads to the prediction that the receptor is oriented in the membrane with a cytoplasmic N- terminus and an extracellular C-terminus.
The EMBO JournalSpecificity of binding of clathrin adaptors to signals on the mannose‐6‐phosphate/insulin‐like growth factor II receptor.
334 Citations1989Jonathan N. Glickman, Elizabeth Conibear +1 more
Mutation of the two tyrosines in the tail of the M6P receptor tail essentially eliminates the interaction with the HA‐II plasma membrane adaptor, which recognizes a ‘tyrosine’ signal on other endocytosed receptors (for example, the LDL receptor and the poly Ig receptor).
The Journal of Cell BiologyRapid stimulation of pinocytosis in human carcinoma cells A-431 by epidermal growth factor.
327 Citations1979Harry T. Haigler, J A McKanna +1 more
Biochemical methods showed that HRP uptake in control cultures was directly proportional to the duration of exposure and the tumor-promoter 12-0- tetradecanoyl-phorbol-13-acetate was effective in stimulating an increase in the rate of pinocytosis.
The Journal of Cell BiologyRole of the human transferrin receptor cytoplasmic domain in endocytosis: localization of a specific signal sequence for internalization.
307 Citations1990Shiyun Jing, T Spencer +3 more
The cytoplasmic domain of the transferrin receptor contains a specific signal sequence located within amino acid residues 19-28 that determines high efficiency endocytosis and Tyr 20 is an important element of that sequence.
CellProtein organization in clathrin trimers
295 Citations1981Tomas Kirchhausen, Stephen C. Harrison
Intact 8.6S clathrin can reassemble into cages without participation of additional protein species.
Journal of Biological ChemistryTopography and subunit stoichiometry of the coated vesicle proton pump.
279 Citations1988Hiroshi Arai, Geronimo Terres +2 more
It is found that the 73,000-dalton polypeptide can be dissociated from the complex with 0.5 M KI in the absence of detergent, indicating a peripheral association of this subunit with the membrane.
CellFc receptor isoforms exhibit distinct abilities for coated pit localization as a result of cytoplasmic domain heterogeneity
263 Citations1989Heini M. Miettinen, John K. Rose +1 more
The mouse macrophages and lymphocytes express two distinct isoforms of a single class of Fc receptor for IgG, and the FcRII-B2 cytoplasmic tail contains a domain needed for accumulation in coated pits, and this domain is disrupted by the 47 amino acid insertion in FcB1.
CellA single amino acid change in the cytoplasmic domain allows the influenza virus hemagglutinin to be endocytosed through coated pits
244 Citations1988Janette Lazarovits, Michael G. Roth
Results indicate a specific and local role for the tyrosine introduced into the cytoplasmic domain of HA that is necessary for interaction of the protein with coated pits.
CellCycling of the integral membrane glycoprotein, LEP100, between plasma membrane and lysosomes: Kinetic and morphological analysis
241 Citations1987Jennifer Lippincott‐Schwartz, Douglas M. Fambrough
The results support the existence of a hitherto unappreciated pathway of membrane flow from lysosomes and should not be considered simply a terminal target of membrane trafficking.
Biochemical and Biophysical Research CommunicationsYeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases
238 Citations1988Kensaku Mizuno, Tomoko Nakamura +3 more
The amino acid sequence of the KEX2 gene product deduced from nucleotide sequencing indicates that the Kex2-encoded protein may function as a membrane-bound, subtilisin-like serine protease.
The Journal of Cell Biology100-kD coated vesicle proteins: molecular heterogeneity and intracellular distribution studied with monoclonal antibodies
236 Citations1987MS Robinson
Two antibodies that recognize both A and C stained fixed and permeabilized tissue culture cells showed a punctate pattern in the plane of the plasma membrane, which may help to explain how different membrane proteins can be sorted into coated vesicles in different parts of the cell.
The Journal of Cell BiologyAssembly polypeptides from coated vesicles mediate reassembly of unique clathrin coats.
224 Citations1983S Zaremba, James H. Keen
AP promotes clathrin reassembly into coat structures of uniform size and distinctive composition under physiologically relevant salt, temperature, and pH conditions and raises the possibility that AP in the cell may be associated with this subpopulation of coat structures.
The Journal of Cell BiologyAssembly and packing of clathrin into coats.
208 Citations1981R.A. Crowther, Barbara M. F. Pearse
A model for the packing of clathrin molecules into the characteristic hexagons and pentagons covering coated pits and vesicles, which reveals details of the structure which correlate well with the predicted features of the model.
The EMBO JournalTransferrin receptor and its recycling in HeLa cells.
206 Citations1982Jeffrey D. Bleil, Mark S. Bretscher
The transferrin receptor is a 180 000‐dalton protein which can be dissociated to two 90 000‐ Dalton polypeptides under reducing conditions and can be labelled by lactoperoxidase‐catalysed iodination on the cell surface at 0 degree C, which causes it to become trypsin resistant.
Proceedings of the National Academy of SciencesCoated pits act as molecular filters.
204 Citations1980Mark S. Bretscher, J. Nichol Thomson +1 more
Two proteins--theta and H63 antigens--are found to be excluded from coated pits on fibroblast plasma membranes, which constitutes part of a lipid flow cycle that was proposed earlier to explain the capping of crosslinked surfaceAntigens.
Annual Review of Biophysics and Biophysical ChemistrySTRUCTURE AND ASSEMBLY OF COATED VESICLES
201 Citations1987Barbara M. F. Pearse, R.A. Crowther
Packing Considerations, three-Dimensional Structure oj the Cage, and The Cytoplasmic Portion oj Receptors: Structure and FUNCTION.
CellDeletion of the cytoplasmic domain of the polymeric immunoglobulin receptor prevents basolateral localization and endocytosis
200 Citations1986Keith E. Mostov, Anne de Bruyn Kops +1 more
The results suggest that the cytoplasmic domain of the receptor is necessary for both basolateral localization and endocytosis.
ScienceA Test of Clathrin Function in Protein Secretion and Cell Growth
198 Citations1985Gregory S. Payne, Randy Schekman
The data presented demonstrate that Saccharomyces cerevisiae does not require clathrin for either cell growth or protein secretion, and protein transport through the secretory pathway is not obligately coupled to the formation of clathin-coated vesicles.
The Journal of Cell BiologyClathrin assembly proteins: affinity purification and a model for coat assembly.
188 Citations1987James H. Keen
Results indicate the existence of a family of assembly proteins within cells and a model for AP-mediated coat assembly is proposed in which a bivalent AP-2 molecule bridges the distal legs or terminal domains of two clathrin trimers that are destined to occupy adjacent vertices in the assembled coat.
The Journal of Cell BiologyCloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins).
157 Citations1989Margaret S. Robinson
In situ hybridization carried out on mouse brain sections indicates that the same cell type may express both transcripts, but that their relative expressions vary.
ScienceClathrin: A Role in the Intracellular Retention of a Golgi Membrane Protein
156 Citations1989Gregory S. Payne, Randy Schekman
Analysis of this defect indicates that the endoprotease Kex2p, which is responsible for initiating proteolytic maturation of the alpha-factor precursor in the Golgi apparatus, is unexpectedly present at the plasma membrane in mutant cells.
Cell46 kd mannose 6-phosphate receptor: Cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor
150 Citations1987Nancy Dahms, Peter Lobel +3 more
Encoding the entire sequence of the bovine 46 kd cation-dependent mannose 6-phosphate (CD Man-6-P) receptor results in a protein that binds specifically to phosphomannan-Sepharose, thus demonstrating that the cDNA clones encode a functional receptor.
NatureClathrin light chains contain brain-specific insertion sequences and a region of homology with intermediate filaments
142 Citations1987Antony P. Jackson, Heng Fong Seow +3 more
The primary structures of four bovine clathrin light chains have been determined and a potentially α-helical region of the clathin light chains shows homology with intermediate filament proteins.
ScienceClathrin Requirement for Normal Growth of Yeast
135 Citations1987Sandra K. Lemmon, Elizabeth W. Jones
Results indicate that clathrin is required for normal growth of yeast, and, therefore, most likely, for growth of all eukaryotic cells.
Journal of Biological ChemistryIdentification of a clathrin binding subunit in the HA2 adaptor protein complex
120 Citations1989S Ahle, Ernst Ungewickell
The results support the conjecture that the beta-type subunits (beta and beta') of the HA2 and HA2 adaptor complexes serve to attach the HA-2 adaptors to clathrin, while the other subunits may determine the specificity of binding to docking proteins and receptors on cytoplasmic membrane surfaces.
Proceedings of the National Academy of Sciences100-kDa polypeptides in peripheral clathrin-coated vesicles are required for receptor-mediated endocytosis.
108 Citations1989Daniel J. Chin, Robert M. Straubinger +3 more
Results show that the 100-kDa polypeptides are directly involved in localized clathrin assembly at the cell periphery and are markers for the endocytic pathway.
The Journal of Cell BiologyAssembled and unassembled pools of clathrin: a quantitative study using an enzyme immunoassay.
107 Citations1985Bruno Goud, C. Huet +1 more
The data support the view that the amount of clathrin (relative to total cell protein) in eucaryotic cells is not related to the extent of receptor-mediated endocytosis and intracellular membrane traffic.
Proceedings of the National Academy of SciencesCloning of a cDNA encoding the human cation-dependent mannose 6-phosphate-specific receptor.
95 Citations1987Regina Pohlmann, Georg Nagel +8 more
Analysis of a panel of somatic cell (mouse-human) hybrids shows that the gene for the receptor is located on human chromosome 12, and the deduced amino acid sequence is colinear with that determined by amino acid sequencing of the N-terminus peptide and nine tryptic peptides.
The Journal of Cell BiologyImmunofluorescent localization of 100K coated vesicle proteins.
93 Citations1986Margaret S. Robinson, Barbara M. F. Pearse
Two antisera were able to detect a 100K band on blots of whole 3T3 cell protein and were used for immunofluorescence, indicating that when vesicles become uncoated, the 100K proteins are removed as well as clathrin.
Journal of Biological ChemistryConservation and diversity in families of coated vesicle adaptins.
93 Citations1990Sreenivasan Ponnambalam, Margaret S. Robinson +3 more
The complete sequence of the beta adaptin subunit of the plasma membrane adaptor complex from coated vesicles has been elucidated and it is found that the 937-amino acidbeta adaptin polypeptide is totally conserved between species.
Journal of Biological ChemistryPurification and characterization of two distinct complexes of assembly polypeptides from calf brain coated vesicles that differ in their polypeptide composition and kinase activities.
85 Citations1987James J. Manfredi, W L Bazari
Two complexes have been identified that differ in their polypeptide composition and kinase activities, but are similar in their ability to assemble clathrin triskelions into cages.
The Journal of Cell BiologyClathrin-coated vesicle assembly polypeptides: physical properties and reconstitution studies with brain membranes.
79 Citations1988David M. Virshup, Vann Bennett
A specific membrane protein binding site for 125I-labeled assembly polypeptides in 0.1 N sodium hydroxide-extracted bovine brain membranes is demonstrated based on the following criteria: binding is displaceable by unlabeled ligand, the binding site is destroyed by protease treatment of the membranes, and the distribution of binding between vesicle-depleted membranes and coated vesicles parallels the in vivo localization of assembly polyPEptides and clath
The EMBO JournalA new method for detecting endocytosed proteins.
78 Citations1988Mark S. Bretscher, René Lutter
The results indicate that intracellular receptors are in non‐reducing compartments, and that uptake of average cell surface (by non‐coated pit processes) in K562 cells is small.
Journal of Biological ChemistryThe flexible region of protein L12 from bacterial ribosomes studied by proton nuclear magnetic resonance
48 Citations1989V.N. Bushuev, A.T. Gudkov +2 more
The dimeric protein L7/L12 from bacterial ribosomes has a highly elongated and flexible structure and residues 37-50 were found to be highly mobile whereas the amino- terminal and COOH-terminal regions are organized into folded domains.
DNAMolecular Cloning and Complete Amino Acid Sequence of AP50, an Assembly Protein Associated with Clathrin-Coated Vesicles
44 Citations1988Christophe Thurieau, Jürgen Brosius +7 more
Comparison with the partially sequenced bovine brain AP50 shows a primary structure that is highly conserved, and AP50 does not have detectable sequence similarity with other known kinases or with other proteins of known sequence.
The EMBO JournalThe mannose 6‐phosphate receptor cytoplasmic domain is not sufficient to alter the cellular distribution of a chimeric EGF receptor.
28 Citations1990Suzanne M. Dintzis, Suzanne R. Pfeffer
It is demonstrated that the MPR cytoplasmic domain is not sufficient to alter the distribution of the EGF receptor, and a role for extracellular and transmembrane domains in MPR routing is suggested.
Trends in Biochemical SciencesPathways of endocytosis in secretory cells
27 Citations1981Volker Herzog
Electron dense tracers applied to the apical cell surfaces of glandular cells reveal that membranes of secretion granules inserted during exocytosis into the plasma membrane are retrieved by endocytoses, pointing to the re-use of retrieved membrane in the secretory cycle.