Base Composition of Deoxyribonucleic Acid of the Temperature-Sensitive Kanamycin-Resistant R Factor, R <i>ts</i> 1
Journal of BacteriologyPublished 1 March 1970Open access
Nobuichi Goto, Yoko Yoshida, Yoshiro Terawaki, Rintaro NAKAYA, Kenji Suzuki
Citations17
SJR quartileQ2
SJR score1.09
SNIP0.85
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TL;DR
The buoyant density of deoxyribonucleic acid (DNA) from the temperature-sensitive R factor, Rts1, was determined by CsCl density-gradient centrifugation, suggesting that RTS1 represents a new molecular class of R factors.
Abstract
The buoyant density of deoxyribonucleic acid (DNA) from the temperature-sensitive R factor, Rts1, was determined by CsCl density-gradient centrifugation. Rts1 was found to consist of a single species of DNA of density 1.705 g/cm(3), which corresponds to a base composition of 45% guanine plus cytosine. This value is distinct from the densities previously reported for other R factors, suggesting that Rts1 represents a new molecular class of R factors.
Keywords
Biochemistry, Genetics and Molecular Biology
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A method has been described for the isolation of DNA from micro-organisms which yields stable, biologically active, highly polymerized preparations relatively free from protein and RNA, and Representative samples have been characterized for their thermal stability and sedimentation behaviour.
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