Genome-wide analysis of developmental and sex-regulated gene expression profiles in <i>Caenorhabditis elegans</i>
Proceedings of the National Academy of SciencesPublished 26 December 2000Open access
Min Jiang, Jubin Ryu, Monika Kiraly, Kyle Duke, V Reinke, Stuart K. Kim
Citations290
SJR quartileQ1
SJR score3.41
SNIP2.38
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TL;DR
These full-genome DNA microarrays are used to show the relative levels of gene expression for nearly every gene during development, from eggs through adulthood, and identify many genes likely to be involved in sex-specific differentiation and behavior.
Abstract
We have constructed DNA microarrays containing 17,871 genes, representing about 94% of the 18,967 genes currently annotated in the Caenorhabditis elegans genome. These DNA microarrays can be used as a tool to define a nearly complete molecular profile of gene expression levels associated with different developmental stages, growth conditions, or worm strains. Here, we used these full-genome DNA microarrays to show the relative levels of gene expression for nearly every gene during development, from eggs through adulthood. These expression data can help reveal when a gene may act during development. We also compared gene expression in males to that of hermaphrodites and found a total of 2,171 sex-regulated genes (P < 0.05). The sex-regulated genes provide a global view of the differences between the sexes at a molecular level and identify many genes likely to be involved in sex-specific differentiation and behavior.
Keywords
Biochemistry, Genetics and Molecular Biology
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