The Recovery of Lethally Irradiated Dogs Given Infusions of Autologous Leukocytes Preserved at -80 C
BloodPublished 1 January 1964Open access
John A. Cavins, STUART C. SCHEER, E. Donnall Thomas, Joseph W. Ferrebee
Citations162
SJR quartileQ1
SJR score5.82
SNIP3.47
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TL;DR
It is concluded that peripheral blood contains primitive cells capable of repopulating marrow spaces and restoring marrow function in mongrel dogs exposed to whole-body irradiation and given intravenous leukocytes that had been previously stored at -80 C.
Abstract
Abstract Nine normal mongrel dogs were exposed to 1200 r whole-body irradiation at 4 to 5 r per minute. They were then given intravenous infusions of 2.12 to 20 x 109 autologous leukocytes that had been previously stored at -80 C. in 10 per cent dimethyl-sulfoxide. Three dogs survived with delayed but complete hematopoietic recovery. Three showed beginning marrow regeneration but died within 3-4 weeks of irradiation. Three given less than 6 x 109 cells died within 21 days. The number of leukocytes infused was critical since there was no survivor among the dogs receiving less than 9 x 109 cells. It is concluded that peripheral blood contains primitive cells capable of repopulating marrow spaces and restoring marrow function.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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