Radioimmunoassay of atrial natriuretic factor: Human plasma levels
Generate an AI Snapshot to get a quick, structured summary of this paper.
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
A simple and sensitive radioimmunoassay procedure has been developed for the determination of atrial natriuretic factor (ANF) in human plasma and showed the presence of one immunoreactive peak of ANF corresponding to its low molecular weight form.
Abstract
A simple and sensitive radioimmunoassay procedure has been developed for the determination of atrial natriuretic factor (ANF) in human plasma. The rabbit antiserum was obtained from a commercial source. ANF was extracted from plasma using an octadecasilyl silica cartridge with a recovery of 78.7%. HPLC of the plasma extract showed the presence of one immunoreactive peak of ANF corresponding to its low molecular weight form. Plasma ANF in humans increased from 8.0 +/- 2.2 in upright position to 20.0 +/- 5.9 fmol/ml (n = 6) in downward position (p less than 0.005).