A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides
GenePublished 1 January 1986
Matty Verlaan–de Vries, Marianne E. Bogaard, Hans van den Elst, Jaques H. van Boom, Alex J. van der Eb, Johannes L. Bos
Citations341
SJR quartileQ2
SJR score0.68
SNIP0.72
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Abstract
To analyze human tumors for the presence of mutated ras oncogenes, a procedure was developed based on selective hybridization of mutation-specific oligodeoxynucleotide probes to genomic DNA [Bos et al., Nucl. Acids Res. 12 (1984) 9155-9163]. We have improved this procedure both in sensitivity and speed by including an in vitro amplification step of ras-specific sequences. This amplification step has first been described by Saiki et al. [Science 230 (1985) 1350-1353] and results in a more than 10(4)-fold increase in the sequence which might contain the mutation. Furthermore, we have improved the selectivity of our hybridizations. As a result, mutated ras oncogenes can now be detected with a dot-blot screening procedure requiring less than 1 microgram of tumor DNA.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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