The Adenovirus EIIA Enhancer
Journal of Biological ChemistryPublished 1 April 1989Open access
Mary R. Loeken, John Brady
Citations99
SJR quartileQ1
SJR score1.71
SNIP1.00
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TL;DR
Using gel shift analysis, it is demonstrated that the binding activity of EIIF is increased following wild-type adenovirus infection and the bindingActivity of ATF is decreased by viral infection.
Abstract
Adenovirus EIIA upstream sequences which contain the binding sites for proteins ATF and EIIF act as an enhancer and can be trans-activated by both E1A and SV40 T/t-antigens. Specific mutation of either the ATF or EIIF binding site demonstrates that both act as positive regulators, decreasing transcription greater than 10-fold. Mutation of both the ATF and EIIF binding sites inhibited the EIIA enhancer 200-fold. Analysis of insertion mutations suggests that the spatial alignment of the upstream ATF and EIIF binding sites with respect to the downstream EIIF binding site on the DNA helix is important. Consistent with previous findings, using gel shift analysis we demonstrate that the binding activity of EIIF is increased following wild-type adenovirus infection. In contrast, using identical gel shift conditions, the binding activity of ATF is decreased by viral infection.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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