Full‐term development of mouse blastomere nuclei transplanted into enucleated two‐cell embryos
Journal of Experimental ZoologyPublished 1 May 1987
Y. Tsunoda, T. Yasui, Y. Shioda, Kae Nakamura, Tsuyoshi Uchida, T. Sugie
Citations144
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TL;DR
It appears that cytoplasmic factors are important for the development of nuclei from advanced cells in enucleated zygotes which contain advanced nuclei.
Abstract
The nuclei from four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos. It was found that such embryos not only developed to the blastocyst stage in vitro (72% and 35%), but also developed to full term (22% and 8%) after transfer to recipient mice. However, development of embryos which contained nuclei from the inner cell mass was not observed. Since the development of enucleated zygotes which contain advanced nuclei is limited (the present study; McGrath and Solter: Science, 226:1317-1319, '84; Robl, Gilligan, Critser, and First: Biol. Reprod., 34:733-739, '86), it appears that cytoplasmic factors are important for the development of nuclei from advanced cells.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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