A SPECIFIC AND SENSITIVE ENZYMATIC ASSAY FOR TRYPTAMINE IN TISSUES
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TL;DR
A sensitive and specific enzymatic-isotopic method for the determination of tryptamine content in animal tissues is described, and the levels of endogenous tryptamines were quantified in rat brain and peripheral tissues.
Abstract
A sensitive and specific enzymatic-isotopic method for the determination of tryptamine content in animal tissues is described. Organ extracts are incubated with S-adenosylmethionine-14C in the presence of an N-methyltransferase, purified from rabbit lung. Under the conditions of the assay N-14C-methyl- and N, N-14C-dimethyltryptamine are the only products extracted in amounts directly proportional to the tissue tryptamine present in the incubation mixture; there is negligible conversion of other amines to radioactive products. With this assay, the levels of endogenous tryptamine were quantified in rat brain and peripheral tissues.