Fractionation of solubilized sarcoplasmic reticulum?
Biochemical and Biophysical Research CommunicationsPublished 1 September 1971
Noriaki Ikemoto, Gopal M. Bhatnagar, J. Gergely
Citations97
SJR quartileQ2
SJR score0.75
SNIP0.56
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TL;DR
A method has been developed which permits the separation of three protein components of the fragmented sarcoplasmic reticular membrane following solubilization with Triton X-100.
Abstract
A method has been developed which permits the separation of three protein components of the fragmented sarcoplasmic reticular membrane following solubilization with Triton X-100. One fraction contains a 100,000 dalton component and constitutes about 20–30% of the total membrane protein; in this fraction the level of ATPase activity and phosphorylated intermediate formation is about four times higher than the level after activation with Triton. Another fraction (62,000 dalton, 20–25% of the total protein) is precipitated upon addition of 4 mM Ca++. The third fraction (125,000–160,000 dalton) constitutes about 50% of the total protein.
Keywords
Biochemistry, Genetics and Molecular Biology
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