An epithelial blood-aqueous barrier to horseradish peroxidase in the ciliary processes of the vervet monkey (Cercopithecus aethiops)
Cell and Tissue ResearchPublished 1 January 1971
Torgeir Vegge
Citations82
SJR quartileQ1
SJR score1.18
SNIP0.94
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TL;DR
Vervet monkeys given horseradish peroxidase intravenously penetrated the capillary walls of the ciliary processes and “filled” the stroma and the intercellular spaces as far as the basal junctional complex of the superficial epithelial cells.
Abstract
Vervet monkeys were given horseradish peroxidase intravenously. Subsequent studies with the electron microscope showed:
Keywords
MedicineNeuroscienceBiochemistry, Genetics and Molecular Biology
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Intradermal, intraperitoneal and intravenous injections of horseradish peroxidase solutions induce increased vascular permeability in rats and this phenomenon occurs also in the guinea pig but is minimal or non-existent in the mouse.
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Investigations on the structure of horseradish peroxidase (donor: H 2 O 2 oxidoreductase, EC 1.11.1.7), including its amino acid and carbohydrate composition, are described.
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It was calculated that the albumin concentration in the tissue fluid of the choroid and in that of the anterior uvea as a whole is more than tenfold the album in concentrate in the aqueous humour and that thealbumin drainage with the aQueous humour can account for less than 10 per cent of the albumIn drainage from the eye.
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The capillaries have a continuous endothelium without fenestrae, and a rather thick basement membrane is present, similar to what is found in the human iris.
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It is concluded that conclusions about the size of functional pores based on the known molecular weight (40000 daltons) are permitted, when the enzyme is used in permeability studies.
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The conclusion is drawn that the ciliary epithelium functions as a barrier to the molecules of the solutes in question, and that it may also have this function in vivo with regard to molecules of similar size.
