Recombinant human interleukin 5 is a selective activator of human eosinophil function.
The Journal of Experimental MedicinePublished 1 January 1988Open access
Angel F. López, Colin J. Sanderson, Jennifer R. Gamble, HD Campbell, IG Young, M A Vadas
Citations959
SJR quartileQ1
SJR score5.83
SNIP1.99
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Human rIL-5 was found to selectively stimulate morphological changes and the function of human eosinophils, and is thus a prime candidate for the selective eOSinophilia and eos inophil activation seen in disease.
Abstract
Human rIL-5 was found to selectively stimulate morphological changes and the function of human eosinophils. This molecule is thus a prime candidate for the selective eosinophilia and eosinophil activation seen in disease.
Keywords
Medicine
Journal of Clinical InvestigationRecombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival.
715 Citations1986Angel F. López, Dominic J. Williamson +8 more
It is shown that granulocyte-macrophage colony stimulating factor can selectively stimulate mature granulocytes function and induced morphological changes and enhanced the survival of both neutrophils and eosinophils by 6 and 9 h, respectively.
BloodBiologic properties in vitro of a recombinant human granulocyte- macrophage colony-stimulating factor
455 Citations1986D Metcalf, C. Glenn Begley +8 more
The Journal of ImmunologyA New Method for the Purification of Human Eosinophils and Neutrophils, and a Comparison of the Ability of These Cells to Damage Schistosomula of <i>Schistosoma Mansoni</i>
358 Citations1979Mathew A. Vadas, John R. David +3 more
Centrifugation of human white blood cells over either Ficoll-Hypaque or slightly hypertonic Metrizamide discontinuous gradients reliably produces separate fractions that are enriched for either neutrophilic or eosinophilic granulocytes.
Proceedings of the National Academy of SciencesMolecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).
325 Citations1987Hugh D. Campbell, William Q. J. Tucker +6 more
Human EDF represents a distinct hemopoietic growth factor that could play a central role in the regulation of eosinophilia and shows strong homology with that of murine EDF.
The Journal of Cell BiologyThe kinetics of chemotactic peptide-induced change in F-actin content, F-actin distribution, and the shape of neutrophils.
312 Citations1985Thomas H. Howard, Christian Oresajo
The results indicate that actin polymerization and depolymerization follow fMLP stimulation in sequence, the rate of depolymersization and the maximum and steady state F-actin content but not the rates of polymerization are fMLLP dose dependent, and concurrent with F- actin depolymization, F-actsin is redistributed and the cell changes shape.
The Journal of ImmunologyEnhancement of human eosinophil cytotoxicity and leukotriene synthesis by biosynthetic (recombinant) granulocyte-macrophage colony-stimulating factor.
281 Citations1986David S. Silberstein, W F Owen +7 more
Pro purified biosynthetic (recombinant) human T lymphocyte granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced markedly two eosinophil functions: cytotoxicity toward schistosomula and calcium ionophore A23187-induced generation of leukotriene C4 (LTC4) by a mean of 135%.
The Journal of ImmunologyActivation of antibody-dependent cell-mediated cytotoxicity of human neutrophils and eosinophils by separate colony-stimulating factors.
244 Citations1983M A Vadas, Nicos A. Nicola +1 more
The results support the notion that human CSF have two sites of action: i) the progenitor cell, where they stimulate a relatively slow process of differentiation, and ii) the mature cell,Where they have a rapid action of increasing functional capacity.
Nucleic Acids ResearchCloning of cDNA for human T-cell replacing factor (interieukin-5) and comparison with the murine homologue
205 Citations1986Chihiro Azuma, Toshizumi Tanabe +10 more
Human interleukin-5 synthesized by the direction of the cloned cDNA induced immunoglobulin synthesis in human B cells stimulated by Staphylococcus aureus mitogen and the sizes of the mature proteins appear similar.
Proceedings of the National Academy of SciencesStimulation of proliferation, differentiation, and function of human cells by primate interleukin 3.
203 Citations1987Angel F. López, Lim To +8 more
GIL-3 has a distinct set of functions and may predict the range of actions of the human molecule among cytokines that are active on human hematopoietic cells, and does not significantly stimulate any of the neutrophil functions tested.
Proceedings of the National Academy of SciencesTumor necrosis factor enhances eosinophil toxicity to Schistosoma mansoni larvae.
174 Citations1986David S. Silberstein, John R. David
TNF can enhance eosinophil function and may constitute an important immunological regulatory mechanism and should be considered when TNF is applied clinically.
The Journal of Experimental MedicineMurine eosinophil differentiation factor. An eosinophil-specific colony-stimulating factor with activity for human cells.
169 Citations1986Angel F. López, C. Glenn Begley +4 more
The possession by EDF (Eo-CSF) of all the properties expected of a human eosinophil CSF raises the possibility that a human analog of this molecule exists, and is involved in the regulation of production and function of human eOSinophils in vivo.
New England Journal of MedicineEnhanced Helminthotoxic Capacity of Eosinophils from Patients with Eosinophilia
139 Citations1980John R. David, Mathew A. Vadas +6 more
It is suggested that eosinophilia involves not only a quantitative change in eOSinophil numbers but also a qualitative change in functional capacity that renders circulating eos inophils more effective in resisting parasitic infections.
Journal of Clinical InvestigationComparison of Human Eosinophils from Normals and Patients with Eosinophilia
133 Citations1980David Bass, William H. Grover +4 more
Electrophoretic mobility, cell surface charge, membrane transport of deoxyglucose, activation of lyso-somal acid phosphatase, and oxidative metabolism were examined to provide a profile to compare EE with purified normal eosinophils (NE) and normal neutrophils.
British Journal of HaematologyThe Association of Eosinophilia with Lymphoblastic Leukaemia or Lymphoma: a Study of Seven Patients
124 Citations1980Daniel Catovsky, Carlo Bernasconi +8 more
Seven patients with hypereosinophilia in association with a lymphoblastic malignancy are described, and cytochemical and ultrastructural studies failed to show any evidence of myeloid differentiation in the blast cells.
PubMedStudies on blood eosinophils. I. Patients with a transient eosinophilia.
96 Citations1976Phang C. Tai, C J Spry
It was concluded that blood eosinophils in patients with an eOSinophilia may be functionally mature or altered in response to unknown stimulae while they are in the blood.
Journal of Clinical InvestigationRapid changes in light scattering from human polymorphonuclear leukocytes exposed to chemoattractants. Discrete responses correlated with chemotactic and secretory functions.
63 Citations1984Itzhak Yuli, Ralph Snyderman
The rapid perpendicular light-scattering response of polymorphonuclear leukocytes to chemoattractants appears to record an initial event in the stimulus-response coupling, and its measurement should provide a useful new tool for the study of leukocyte function.
NatureEosinophils and not lymphoid K cells kill Trypanosoma cruzi epimastigotes
49 Citations1977Colin J. Sanderson, Angel F. López +1 more
It is shown that K cells in rat spleen have no detectable activity against T. cruzi, but that the activity resides in cell preparations rich in eosinophils, which is important biologically in Chagas' disease.
CancerLarge cell carcinoma of the lung associated with marked eosinophilia. A case report
46 Citations1984Tetsuro Kodama, Kenichi TAKADA +4 more
Eosinophil colony stimulating factor production by the transplanted tumor in a nude mouse was confirmed by use of a human bone marrow culture assay system, and chemotacticfactor production by metastatic tumor tissue also was proved by a modified microfilter technique.
InflammationUnique characteristics of superoxide production by human eosinophils in eosinophilic states
45 Citations1979Alfred I. Tauber, Edward J. Goetzl +1 more
The prolonged release of O2− by eosinophils engaged in phagocytosis may contribute both to their unique microbicidal profile and to the capacity of eos inophils to injure host tissues in some eosInophilic syndromes.
Heart and VesselsThe pathogenesis of eosinophilic endomyocardial disease in patients with carcinomas of the lung
14 Citations1985C J Spry, A P Weetman +3 more
Findings confirm the suggestion that the presence in the blood of >1×109/l degranulated eosinophils can be used to predict the development of eosInophilic endomyocardial disease before it becomes apparent clinically, and add weight to the hypothesis that blood eos inophil degranulation causes this complication of hypereosinophile states.