A Reverse Transcriptase-PCR Assay for Detecting Filarial Infective Larvae in Mosquitoes
PLoS neglected tropical diseasesPublished 17 June 2008Open access
Sandra J. Laney, Caitlin J. Buttaro, S Visconti, Nils Pilotte, Reda M. R. Ramzy, Gary J. Weil
Citations40
SJR quartileQ1
SJR score1.37
SNIP1.44
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TL;DR
This L3-activated gene transcript, combined with a control transcript that is constitutively expressed by all vector-stage filarial larvae, can be used to detect filarial infectivity in pools of mosquito vectors.
Abstract
This L3-activated gene transcript, combined with a control transcript (tph-1, accession # U80971) that is constitutively expressed by all vector-stage filarial larvae, can be used to detect filarial infectivity in pools of mosquito vectors. This general approach (detection of stage-specific gene transcripts from eukaryotic pathogens) may also be useful for detecting infective stages of other vector-borne parasites.
Keywords
Agricultural and Biological SciencesMedicineEnvironmental Science
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