Sites of action potential generation in cultured vertebrate neurons
Brain ResearchPublished 1 December 1983
Thomas G. Smith
Citations12
SJR quartileQ2
SJR score0.85
SNIP0.72
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TL;DR
The somata of mouse tissue cultured mouse dorsal root ganglion cells and spinal cord neurons and the dendrites of spinal Cord neurons do not actively generate action potentials.
Abstract
The somata of mouse tissue cultured mouse dorsal root ganglion cells and spinal cord neurons and the dendrites of spinal cord neurons do not actively generate action potentials.
Keywords
Neuroscience
Pflügers Archiv - European Journal of PhysiologyThe extracellular patch clamp: A method for resolving currents through individual open channels in biological membranes
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The current contributions of individual ionic channels can be measured by electrically isolating a small patch of membrane by bringing the tip of a small pipette into close contact with an enzymatically cleaned membrane of a hypersensitive amphibian or mammalian muscle fiber.
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It is suggested that combined Ca2+‐Na+ spikes observed in isolated sensory neurones in vitro reflect the action potential of adult sensory cells but the possibility that they represent an early stage in development is also discussed.
Journal of NeuroscienceLocalization of sodium channels in cultured neural cells
137 Citations1981WA Catterall
Visual observation indicated that 37 +/- 5% of cultured spinal cord neurons had a higher sodium channel density on the initial segment of one or more neurites than on the cell body, which may be the basis of the lower threshold for action potential generation at the axonInitial segment of motor neurons and some spinal interneurons in vivo.
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The unresponsiveness of the neurons to TTX was shown to be due to the insensitivity of Na as well as Ca components of action potentials to the toxin.
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The ionic components of the action potentials of mouse spinal cord (SC) cells and dorsal root ganglion (DRG) cells were studied in dissociated cell cultures and it was concluded that the action possibles of SC cells result mainly from a TTX-sensitive Na component.
Journal of NeurophysiologyMouse spinal cord in cell culture. II. Synaptic activity and circuit behavior
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Only one case of electrical coupling between neurons was found, and statistical analysis of evoked excitatory postsynaptic potentials indicates that the quantal size was 200-250 muV, which was below the noise level of the recording system used.
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The capability of membranes to carry Ca currents, as represented by the maximum rate of rise (MRR) of a Ca spike, is enhanced during a particular period of the cell culture, whereas there is no significant change in Na currents.
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While both somatic and axonal membranes of a giant neuron in Aplysia have sodium spike channels, only in the axon is an action potential generated and the sodium channel density in the soma is too low to generate action potentials.
