The nucleotide sequence of a human protamine 1 cDNA
Nucleic Acids ResearchPublished 1 January 1987
Chul‐Hoon Lee, Sigrid Hoyer‐Fender, Wolfgang Engel
Citations37
SJR quartileQ1
SJR score7.78
SNIP4.71
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TL;DR
cDNA human protamine human testis specific cDNA in vector Agt re-gion polyadenylation and its role in infectious disease is still under investigation.
Abstract
Chul-Hoon Lee, Sigrid Hoyer-Fender, Wolfgang Engel; The nucleotide sequence of a human protamine 1 cDNA, Nucleic Acids Research, Volume 15, Issue 18, 25 Se
Keywords
Immunology and MicrobiologyMedicine
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106 Citations1986Hubert Ammer, Agnes Henschen +1 more
The two protamines of human sperm cell nuclei, P1 and P2, were isolated in pure form after extraction with 6M guanidine/5% mercaptoethanol and alkylation with vinyl pyridine by reversed-phase high-performance liquid chromatography and showed a novel type of structure.
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The results show that bovine protamine is 50 amino-acid residues in length and not 47 residues as previously published, and this insert encodes a polypeptide of 50 amino acids of which 26 are arginine, 7 are cysteine, and 2 are tyrosine.
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The results indicate that the protamine gene in the bull is postmeiotically expressed and the mRNA is synthesized as a 680 nucleotide long molecule.