Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
Proceedings of the National Academy of SciencesPublished 1 August 1978Open access
J G Sutcliffe
Citations783
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TL;DR
The nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase, is determined and the first 23 amino acids presumably form a signal for secretion.
Abstract
I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
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