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A rapid micrqpreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells

Nucleic Acids ResearchPublished 1 January 1991Open access
Nancy C. Andrews, Douglas V. Faller
Citations2,338
SJR quartileQ1
SJR score7.78
SNIP4.71
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TL;DR

The aim in developing this micropreparation procedure was to easily and rapidly extract DNA-binding proteins from small numbers of cells, and it gives an excellent yield, comparable to that of the large scale Dignam protocol with minimal proteolysis.

Abstract

Nancy C. Andrews, Douglas V. Faller; A rapid micrqpreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells, Nu

Keywords

Biochemistry, Genetics and Molecular Biology