Basic helix-loop-helix genes in neural development
Current Opinion in NeurobiologyPublished 1 February 1997
Jacqueline E. Lee
Citations518
SJR quartileQ1
SJR score2.54
SNIP1.27
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TL;DR
Several major advances in the understanding of the regulation of vertebrate neurogenesis by members of the basic helix-loop-helix (bHLH) protein family have been made, suggesting a regulatory pathway.
Abstract
Several major advances in the understanding of the regulation of vertebrate neurogenesis by members of the basic helix-loop-helix (bHLH) protein family have been made in the past year. Specifically, a number of bHLH genes have been cloned and shown to convert non-neuronal fate to neuronal fate when expressed ectopically. In particular, studies on NeuroD and Neurogenin suggest a regulatory pathway, providing powerful molecular tools to study vertebrate neurogenesis.
Keywords
Biochemistry, Genetics and Molecular Biology
CellThe protein Id: A negative regulator of helix-loop-helix DNA binding proteins
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CellInteractions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence
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CellThe MyoD family and myogenesis: Redundancy, networks, and thresholds
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CellMammalian achaete-scute homolog 1 is required for the early development of olfactory and autonomic neurons
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ScienceConversion of <i>Xenopus</i> Ectoderm into Neurons by NeuroD, a Basic Helix-Loop-Helix Protein
1,027 Citations1995Jacqueline E. Lee, Stanley M. Hollenberg +4 more
The data suggest that neuroD may participate in the terminal differentiation step during vertebrate neuronal development and seems competent to bypass the normal inhibitory influences that usually prevent neurogenesis in ventral and lateral ectoderm.
Genes & DevelopmentExpression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1,022 Citations1994Dan Turner, H Weintraub
Analysis of molecular markers for neural, epidermal, and neural crest cells indicates that CNS expansion occurs as early as neural plate formation, and inhibition of DNA synthesis shows that additional CNS tissue does not depend on cell division--rather it reflects conversion of prospective neural crest andEpidermal cells to a neural fate.
CellIdentification of neurogenin, a Vertebrate Neuronal Determination Gene
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CellThe T Cell Leukemia Oncoprotein SCL/tal-1 Is Essential for Development of All Hematopoietic Lineages
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Genes & DevelopmentTargeted disruption of mammalian hairy and Enhancer of split homolog-1 (HES-1) leads to up-regulation of neural helix-loop-helix factors, premature neurogenesis, and severe neural tube defects.
699 Citations1995Masami Ishibashi, S L Ang +4 more
Results suggest that HES-1 normally controls the proper timing of neurogenesis and regulates neural tube morphogenesis.
Genes & DevelopmentTwo mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split.
675 Citations1992Yoshiki Sasai, Ryoichiro Kageyama +3 more
The results suggest that HES-1 may play an important role in mammalian development by negatively acting on the two different sequences while Hes-3 acts as a repressor in a specific type of neurons.
NaturePrimary neurogenesis in Xenopus embryos regulated by a homologue of the Drosophila neurogenic gene Delta
674 Citations1995Ajay Chitnis, Domingos Henrique +3 more
Results indicate that the X-Delta-1 protein mediates lateral inhibition delivered by prospective neurons to adjacent cells, and that commitment to a neural fate in vertebrates is regulated by Delta-Notch signalling as in Drosophila.
Genes & DevelopmentTissue-specific regulation of the insulin gene by a novel basic helix-loop-helix transcription factor.
607 Citations1995Francisco J. Naya, Christine M. Stellrecht +1 more
A modified yeast two-hybrid system was utilized to clone a novel bHLH factor, BETA2 (beta-cell E-box trans-activator 2), from a hamster insulin tumor (HIT) cell cDNA library, and northern analysis demonstrates that high-level expression of the BETA 2 gene is restricted to pancreatic alpha- and beta-cell lines.
CellGroucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins
562 Citations1994Ze’ev Paroush
It is proposed that gro is a transcriptional corepressor recruited to specific target promoters by hairy-related bHLH proteins, and this association reflects in vivo maternal requirements for gro during neurogenesis, segmentation, and sex determination, three processes regulated by the above b HLH proteins.
ScienceA Subclass of bHLH Proteins Required for Cardiac Morphogenesis
551 Citations1995Deepak Srivastava, Peter Cserjesi +1 more
Molecular and Cellular Neuroscienceneurogenins,a Novel Family ofatonal-Related bHLH Transcription Factors, Are Putative Mammalian Neuronal Determination Genes That Reveal Progenitor Cell Heterogeneity in the Developing CNS and PNS
547 Citations1996Lukas Sommer, Qiufu Ma +1 more
Two additional ngn's are identified, ngn2 and ngn3, which together define a novel subfamily of atonal-related mouse genes, which represent both a family of putative mammalian neuronal determination genes and useful markers of the origins of neuronal diversity.
NatureTwo rat homologues of Drosophila achaete-scute specifically expressed in neuronal precursors
532 Citations1990Jane E. Johnson, Susan J. Birren +1 more
From a sympathetic neuronal precursor cell line derived from the neural crest, two different mammalian genes that are homologous to genes of the achaete-scute complex are isolated.
Genes & DevelopmentMammalian achaete-scute homolog 1 is transiently expressed by spatially restricted subsets of early neuroepithelial and neural crest cells.
446 Citations1991Lee‐Chiang Lo, Jane E. Johnson +3 more
The data support the idea that helix-loop-helix proteins may represent an evolutionarily conserved family of cell-type determination genes, of which MASH1 is the first neural-specific member identified in vertebrates.
CellHLH proteins, fly neurogenesis, and vertebrate myogenesis
418 Citations1993Yuh Nung Jan, Lily Yeh Jan
The early events of Drosophila neurogenesis are characterized extensively in the wing imaginal disc, which gives rise to the wing and the thorax of the fruit fly.
Mechanisms of DevelopmentDynamic expression of the murine Achaete-Scute homologue Mash-1 in the developing nervous system
413 Citations1993François Guillemot, Alexandra L. Joyner
Its expression pattern suggest that Mash-1 plays a role at early stages of development of specific neural lineages in most regions of the central nervous system and of several linesages in the peripheral nervous system.
DevelopmentScleraxis: a basic helix-loop-helix protein that prefigures skeletal formation during mouse embryogenesis
397 Citations1995Peter Cserjesi, Doris Brown +6 more
The expression pattern, DNA-binding properties and transcriptional activity of scleraxis suggest that it is a regulator of gene expression within mesenchymal cell lineages that give rise to cartilage and connective tissue.
The EMBO JournalPersistent expression of helix‐loop‐helix factor HES‐1 prevents mammalian neural differentiation in the central nervous system.
392 Citations1994Masami Ishibashi, Koki Moriyoshi +4 more
Results show that persistent expression of HES‐1 severely perturbs neuronal and glial differentiation in the developing mammalian central nervous system.
CellExpression of an extracellular deletion of Xotch diverts cell fate in Xenopus embryos
365 Citations1993Clark R. Coffman, Paul Skoglund +2 more
Xotch is a Xenopus homolog of Notch, a receptor involved in cell fate decisions in Drosophila, and an extracellular deletion construct is shown that Xotch has a similar role in Xenopus embryos.
Journal of Biological ChemistryA Mammalian Helix-Loop-Helix Factor Structurally Related to the Product of Drosophila Proneural Gene atonal Is a Positive Transcriptional Regulator Expressed in the Developing Nervous System
359 Citations1995Chihiro Akazawa, Mākoto Ishibashi +3 more
The results suggest that MATH-1 may be a target of HES-1 and play a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
Journal of Biological ChemistryMolecular characterization of a rat negative regulator with a basic helix-loop-helix structure predominantly expressed in the developing nervous system.
305 Citations1992Chihiro Akazawa, Yoshiki Sasai +2 more
The cDNA cloning and characterization of a rat basic helix-loop-helix (HLH) factor, designated HES-5, and its prominent expression in the ventricular zones of the embryonal brain vesicles and the outer nuclear layer of the neural retina suggest that the negative regulator HES
Genes & DevelopmentCell interactions and gene interactions in peripheral neurogenesis.
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This work has revealed that a succession of steps leads from simple anteroposterior heterogeneities in the distribution of a few key factors present in the oocyte to the reproducible establishment of 14 consecutive metameres shortly after cellularization of the embryo.
Developmental BiologyDermo-1: A Novel Twist-Related bHLH Protein Expressed in the Developing Dermis
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The expression pattern of Dermo-1 suggests that it functions as a regulator of gene expression in a subset of mesenchymal cell lineages including developing dermis, and repressed transcriptional activity of myogenic bHLH proteins.
Developmental BiologyRestricted Expression of a Novel Murineatonal-Related bHLH Protein in Undifferentiated Neural Precursors
253 Citations1996Gérard Gradwohl, Carol Fode +1 more
A novel bHLH protein related to atonal, named MATH4A, is isolated, which is broadly expressed in neural precursor cells in the mouse embryonic CNS and PNS, and its structure and expression pattern suggest that MATH 4A may regulate an early step of neural development, either as a partner of ubiquitous b HLH proteins or associated with other neural-specific bHLh proteins.
The EMBO JournalTranscriptional activation by heterodimers of the achaete‐scute and daughterless gene products of Drosophila.
240 Citations1991Carlos V. Cabrera, María C. Alonso
Embryos deficient for the lethal of scute gene fail to activate hunchback in some neural lineages in a pattern consistent with the lack of a member of a multigene family.
Developmental BiologyParaxis: A Basic Helix-Loop-Helix Protein Expressed in Paraxial Mesoderm and Developing Somites
206 Citations1995Rob Burgess, Peter Cserjesi +2 more
The cloning of a novel basic helix-loop-helix (bHLH) protein, called paraxis, which is nearly identical to scleraxis within the bHLH region but diverges in its amino and carboxyl termini is reported.
DevelopmentSensitivity of proneural genes to lateral inhibition affects the pattern of primary neurons in <i>Xenopus</i> embryos
175 Citations1996Ajay Chitnis, Chris Kintner
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Molecular and Cellular Biology<i>neuroD2</i> and <i>neuroD3</i> : Distinct Expression Patterns and Transcriptional Activation Potentials within the <i>neuroD</i> Gene Family
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The noncongruent expression pattern and target gene specificity of these highly related neurogenic bHLH proteins make them candidates for conferring specific aspects of the neuronal phenotype.
ScienceAcquisition of myogenic specificity by replacement of three amino acid residues from MyoD into E12
149 Citations1992Ronald L. Davis, H Weintraub
It is shown that only two amino acids in the MyoD basic region and a single amino acid from the junction are sufficient for myogenic specificity when substituted into the corresponding region of E12.
Proceedings of the National Academy of SciencesMolecular characterization of NSCL, a gene encoding a helix-loop-helix protein expressed in the developing nervous system.
135 Citations1992C. Glenn Begley, Stanley Lipkowitz +6 more
The very restricted pattern of NSCL expression suggests an important role for this HLH protein in neurological development, and the NSCL gene was mapped to murine chromosome 1.
Development<i>XASH-3</i>, a novel <i>Xenopus achaete-scute</i> homolog, provides an early marker of planar neural induction and position along the mediolateral axis of the neural plate
124 Citations1993Kathryn A. Zimmerman, John Shih +3 more
The restrictedmediolateral expression of XASH-3 suggests that mediolateral patterning of the neural plate is an early event, and that this regionalization can be achieved in the absence of inducing signals derived from underlying mesoderm.
Journal of NeurobiologyMechanisms of early neurogenesis in <i>Drosophila melanogaster</i>
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Experimental evidence indicates that cellular interactions determine which cells will adopt each one of these fates, and that a signal with neuralising character is required to stabilize the primary neural fate in 25% of all the neuroectodermal cells, which will develop as neuroblasts.
Development<i>XASH</i> genes promote neurogenesis in <i>Xenopus</i> embryos
116 Citations1994Beatriz Ferreiro, Chris Kintner +3 more
The results show that the XASH-3 promotes neural development, and suggest that its activity depends on additional factors which are induced in ectoderm by factors such as noggin.
Mechanisms of DevelopmentNEX-1: a novel brain-specific helix-loop-helix protein with autoregulation and sustained expression in mature cortical neurons
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This work has identified a novel bHLH gene, termed NEX-1, which is expressed exclusively and abundantly in the mammalian central nervous system, and suggests that positive autoregulation stabilizes the activity of Nex-1 and its target genes in subsets of mature neurons.
Developmental BiologyThe gene for the helix-loop-helix protein, Id, is specifically expressed in neural precursors
114 Citations1992Melinda K. Duncan, Emanuel DiCicco‐Bloom +3 more
Id, a negative regulator of bHLH proteins, was found to exhibit peak gene expression during mouse embryogenesis with a striking pattern in the central nervous system, and this protein, similar to its Drosophila homolog, extramacrochaetae, may play a negative regulatory role in neural differentiation.
European Journal of BiochemistryMATH‐2, a Mammalian Helix‐Loop‐Helix Factor Structurally Related to the Product of<i>Drosophila</i>Proneural Gene<i>atonal</i>, is Specifically Expressed in the Nervous System
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The molecular characterization of a novel mouse HLH factor, designated MATH-2, that has a structural homology to the product of Drosophila proneural gene atonal is reported, raising the possibility that Math-2 may be a trans-acting factor involved in the development and maintenance of the mammalian nervous system.
Developmental BiologyNeuronal Expression of Regulatory Helix-Loop-Helix Factor Id2 Gene in Mouse
105 Citations1993Toomas Neuman, A. Keen +4 more
The data indicate that Id2 has different functions in different cell types during neurogenesis and in the adult, and that the region which is responsible for the down regulation of transcription during neuronal differentiation is identified.
The EMBO JournalMyf‐5 and myoD genes are activated in distinct mesenchymal stem cells and determine different skeletal muscle cell lineages.
104 Citations1996Thomas Braun, Hans-Henning Arnold
It is demonstrated that skeletal musculature in vertebrates develops from two separate cell lineages and complementation may occur at the cellular level, but not between different myogenic factor genes within one cell.
Mechanisms of DevelopmentXASH1, a Xenopus homolog of achaete-scute: a proneural gene in anterior regions of the vertebrate CNS
92 Citations1993Beatriz Ferreiro, Paul Skoglund +3 more
The combined evidence described in this paper strongly suggests that XASH1 plays a role in Xenopus neurogenesis similar to that played by the ASC genes in Drosophila.
PubMedNSCL-2: a basic domain helix-loop-helix gene expressed in early neurogenesis.
61 Citations1992Gobel, Stanley Lipkowitz +2 more
The temporally and tissue restricted pattern of expression of this gene and its identification as a member of a family of transcription factors relevant to growth and development in a wide variety of species suggest a role for NSCL-2 in the development of the eukaryotic nervous system.
Biochemical and Biophysical Research CommunicationsMolecular Cloning and Characterization of a cDNA Encoding a Novel Basic Helix-Loop-Helix Protein Structurally Related to NeuroD/BHF1
45 Citations1996Michio Yasunami, Kazuo Suzuki +5 more
The structure and expression patterns of NDRF are similar to but distinct from those of NeuroD and MATH-2/NEX-1, suggesting that NDRf may play distinct roles in neural development and plasticity as a novel member of the NeuroD family.
Biochemical and Biophysical Research CommunicationsMolecular Cloning of a Novel Basic Helix-Loop-Helix Protein from the Rat Brain
42 Citations1996Hideaki Kume, Kei Maruyama +4 more
A novel bHLH protein in rat brain is described, which has a b HLH domain highly homologous with that of MATH-2 and NeuroD and was detected only in neural tissue, indicating that it has an important role specific to neuronal activities.