Basal Localization of the Presumptive Auxin Transport Carrier in Pea Stem Cells
SciencePublished 17 June 1983
Mark Jacobs, Scott F. Gilbert
Citations178
SJR quartileQ1
SJR score10.42
SNIP6.62
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
Abstract
By means of an indirect immunofluorescence technique with the use of monoclonal antibodies, the location of the presumptive auxin transport carrier of pea stem tissue was identified in the plasma membranes at the basal ends of parenchyma cells sheathing the vascular bundles. The results represent what is believed to be the first direct evidence for the hypothesized basal efflux carrier conferring polarity to auxin transport in plant stems.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
NatureContinuous cultures of fused cells secreting antibody of predefined specificity
17,158 Citations1975Georges Köhler, César Milstein
The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Annual Review of Plant PhysiologyThe Polar Transport of Auxin
504 Citations1977Mary Helen M. Goldsmith
Theory of the determinants of auxin transport, and some alternative Hypotheses also Involving Diff usion are presented.
New PhytologistTRANSPORT OF INDOLEACETIC ACID IN PLANT CELLS IN RELATION TO pH AND ELECTRICAL POTENTIAL GRADIENTS, AND ITS SIGNIFICANCE FOR POLAR IAA TRANSPORT
351 Citations1975John A. Raven
The apparent active efflux of IAA from these cells can be explained in terms of passive driving forces of concentration and electrical potential acting on IAA and IAA-, with the distribution of these two species dictated ultimately by PIAA, PIAa-) the pH of the various compartments and the electrical potential difference between them.
PLANT PHYSIOLOGYAuxin-binding Sites of Maize Coleoptiles Are Localized on Membranes of the Endoplasmic Reticulum
225 Citations1977Peter M. Ray
The results raise the possibility that primary auxin action occurs at ER membranes and could represent facilitation of the transfer of hydrogen ions and nascent secretory protein into the ER lumen followed by secretory transport of these products to the cell exterior via the Golgi system.
PlantaN-1-napthylphthalamic-acid-binding activity of a plasma membrane-rich fraction from maize coleoptiles
139 Citations1971Carole A. Lembi, D. J. Morr� +2 more
Results are consistent with the localization of auxin transport sites at the plasma membrane of plant cells and appear to be strong KM between 10-8 and 10-7 M) but non-covalent.
Journal of Experimental BotanyA Microautoradiographic Study of Auxin Transport in the Stem of Intact Pea Seedlings (<i>Pisum sativum</i>L.)
88 Citations1978D. A. Morris, A. G. Thomas
Results are consistent with the conclusions drawn from earlier transport experiments which indicated that in the intact plant the long-distance basipetal transport of auxin from the apical bud takes place in a system which is separated from the phloem transport system and suggests that the vascular cambium and its immediate derivatives may function as the normal pathway for the long distance movement of Auxin in the plant.
PlantaAuxin uptake and action of N-1-naphthylphthalamic acid in corn coleoptiles
77 Citations1981Michael R. Sussman, Mary Helen M. Goldsmith
The results support the general hypothesis that cellular auxin uptake and polar transport through tissues are chemiosmotically coupled to the electrochemical potential of auxin and protons.
PlantaThe action of specific inhibitors of auxin transport on uptake of auxin and binding of N-1-naphthylphthalamic acid to a membrane site in maize coleoptiles
69 Citations1981Michael R. Sussman, Mary Helen M. Goldsmith
Using both 1-mm segments of corn coleoptiles and a preparation of membranes isolated from the same source, the effectiveness of several inhibitors of geotropism and polar transport in stimulating uptake of auxin into the tissue and in competing with N-1-naphthylphthalamic acid for a membrane-bound site is compared.
PlantaA saturable site responsible for polar transport of indole-3-acetic acid in sections of maize coleoptiles
56 Citations1982Mary Helen M. Goldsmith
The results fully substantiate the hypothesis that auxin transport consists of a saturable flux of auxin anions (A-) in parallel with a nonsaturably flux of undissociated IAA (HA), with both fluxes operating down their respective concentration gradients.
PlantaNature of cell-to-cell transfer of auxin in polar transport
44 Citations1976W. Z. Cande, Peter M. Ray
The findings are compatible with the traditional model of polar transport as passive uptake combined with an active basal efflux pump for IAA, or with the model of purely passive polar transport driven by pH and/or potential differences across the plasma membrane, provided certain ad hoc assumptions are made about the characteristics of the IAA anion carrier.
PLANT PHYSIOLOGYSolubilization of the Receptor for <i>N</i>-1-Naphthylphthalamic Acid
41 Citations1980Michael R. Sussman, Gary Gardner
Observations of an affinity of auxin analogs for the solubilized NPA receptor raise the interesting possibility that the NPA binding site is one conformation of an auxin binding site involved in polar auxin transport.
PlantaIntracellular localization of the active process in polar transport of auxin
36 Citations1973Mary Helen M. Goldsmith, Peter M. Ray
Experiments indicate that the basipetal transport of auxin is independent of both the position and pressure exerted by the amyloplasts but is strongly dependent on the amount of cytoplasm at the basal end of the cells.
New PhytologistTHE PATHWAY OF TRANSPORT OF APPLIED INDOLYL‐ACETIC ACID THROUGH INTERNODE SEGMENTS
35 Citations1974ELISABETH WANGERMANN
Helianthus internode segments were fitted with apical donor blocks containing [14C]IAA and [3H]sucrose, and plain agar basal receiver blocks, and it was concluded that active polar transport of IAA takes place in or near thephloem, but by a different mechanism from the one involved in sucrose transport through the phloem of intact plants.
Plant Science LettersIsolation of membranes from sugarcane cell suspensions: Evidence for a plasma membrane enriched fraction
24 Citations1975M. Thom, W. M. Laetsch +1 more
Proceedings of the Royal Society of London. Series B, Biological sciencesThe effect of intracellular geometry on auxin transport - II. Geotropism in shoots
8 Citations1981Graeme Mitchison
Calculations are given to support the proposed mechanism by which statolith movement could influence auxin transport, and show that a large enough downward diversion of auxin could be achieved if the permeability of the tonoplast is not too large.
Physiologia PlantarumLe transport basipète de l'AIA‐5‐<sup>3</sup>H dans le coléoptile de blé
4 Citations1982Gérald Perbal, D. Driss‐Ecole +1 more
Results support the theory of polar diffusion of auxin afterBasipetal transport of [5-3H] IAA in the wheat coleoptile showed that 3H-IAA was transported more rapidly than its tritiated metabolites.