An erythroid specific enhancer upstream to the gene encodin the cell-type specific transcription factor GATA-1
Nucleic Acids ResearchPublished 1 January 1991Open access
Silia Nicolis, C Bertini, Antonella Ronchi, Stefania Crotta, Luisa Lanfranco, Emanuela Moroni
Citations78
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The mouse and human GATA-1 genes are cloned and a region upstream to the first exon, and highly conserved between mouse and man, acts as an erythroid specific enhancer in transient assays, if linked to the Gata-1 or to the SV40 promoter.
Abstract
The transcription factor GATA-1 is expressed in a subset of hemopoietic cells, where it mediates the cell-type specific expression of several genes. We have cloned the mouse and human GATA-1 genes. A region upstream to the first exon, and highly conserved between mouse and man, acts as an erythroid specific enhancer in transient assays, if linked to the GATA-1 or to the SV40 promoter. The activity of the enhancer is almost completely dependent on the integrity of a dimeric GATA-1 binding site.
Keywords
Biochemistry, Genetics and Molecular Biology
Molecular Cloning: A Laboratory Manual 2
129,982 Citations1989Joseph Sambrook, Elisabeth Fritsch +1 more
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
Nucleic Acids ResearchAccurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
11,610 Citations1983John David Dignam, Russell M. Lebovitz +1 more
A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Molecular and Cellular BiologyRecombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
7,757 Citations1982C Gorman, Lindsey Moffat +1 more
Molecular and Cellular BiologyRecombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells
3,106 Citations1982Cornelia M. Gorman, Leslie F. Moffat +1 more
A series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells.
SciencePurification and Biochemical Characterization of the Promoter-Specific Transcription Factor, Sp1
1,486 Citations1986Michael R. Briggs, James T. Kadonaga +2 more
Isolation and renaturation of proteins purified from sodium dodecyl sulfate polyacrylamide gels allowed the identification of two polypeptides as those responsible for recognizing and interacting specifically with the GC-box promoter elements characteristic of Sp1 binding sites.
NatureErythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1
1,347 Citations1991Larysa Pevny, M C Simon +6 more
The disruption of the X-linked GATA-1 gene by homologous recombination in a male (XY) murine embryonic stem cell line and testing the Gata-1-deficient cells for their ability to contribute to different tissues in chimaeric mice demonstrates that GATA, the zinc-finger transcription factor, is required for the normal differentiation of erythroid cells, and that other GATAS cannot compensate for its absence.
NatureA nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes
1,153 Citations1986Harinder Singh, Ranjan Sen +2 more
It is reported here the identification of a human B-cell nuclear factor (IgNF-A) that binds to DNA sequences in the upstream regions of both the mouse heavy and κ light-chain gene promoters and also to the mouseHeavychain gene enhancer.
GeneA simple phase-extraction assay for chloramphenicol acyltransferase activity
984 Citations1988Brian Seed, Jenq-Yunn Sheen
A simple and convenient phase extraction assay for chloramphenicol (Cm) acetyltransferase (CAT) activity has been developed, based on the enzymatic butyrylation of radiolabelled Cm, which is highly sensitive, and substantially less expensive than all presently available alternatives.
NatureCloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells
929 Citations1989Shih‐Feng Tsai, David I. K. Martin +4 more
Genes expressed in erythroid cells contain binding sites for a cell-specific factor believed to be an important regulator for this haematopoietic lineage, and complementary DNA encoding the murine protein is identified using high-level transient expression in mammalian cells.
ScienceActivation of the AIDS Retrovirus Promoter by the Cellular Transcription Factor, Sp1
707 Citations1986Katherine A. Jones, James T. Kadonaga +2 more
Findings suggest that one important component of the AIDS virus transcriptional control region interacts with a cellular transcription factor, Sp1, and that this factor must function in conjunction with transcriptional elements located downstream of the RNA cap site to mediate the response of the LTR to viral trans-activation.
Nucleic Acids ResearchAbsolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a<i>P</i>CR aided<i>t</i>ranscipt<i>t</i>itration assay (PATTY)
656 Citations1989Michael Becker-André, Klaus Hahlbrock
A PCR aided transcript titration assay (PATTY) which is based on the co-amplification of an in vitro generated transcript differing by a single base exchange from the target mRNA which gives accurate results within 24 hours and is useful especially for the quantification of either low-abundance mRNA or more abundant mRNA present in very small amounts of total RNA.
Genes & DevelopmentActivity and tissue-specific expression of the transcription factor NF-E1 multigene family.
624 Citations1990Masayuki Yamamoto, L J Ko +4 more
The data suggest that the NF-E1 consensus motif directs a broader and more complicated array of developmental transcriptional regulatory processes than has been assumed and that NF- E1c may play a unique regulatory role in the developing chicken brain and in T lymphocytes.
NatureExpression of an erythroid transcription factor in megakaryocytic and mast cell lineages
472 Citations1990David I. K. Martin, Leonard I. Zon +2 more
It is shown that GF-1 is expressed in two other haematopoietic lineages, megakaryocytes and bone marrow-derived mast cells, and these findings are consistent with results from haem atopoetic progenitor culture which suggest a relationship between erythroid,Megakaryocytic and mast cell lineages and imply thatGF-1 was expressed in committed multipotential cells and their progeny.
NatureMegakaryocytic and erythrocytic lineages share specific transcription factors
439 Citations1990Paul‐Henri Roméo, Marie‐Hélène Prandini +6 more
The data strengthen the idea of a close association between the erythrocytic and the megakaryocytic lineages and could also explain the expression of markers specific to the reythroid-specific and megakarian-oblastic permanent cell lines in most eries.
Genes & DevelopmentThe human beta-globin gene 3' enhancer contains multiple binding sites for an erythroid-specific protein.
359 Citations1988Lee Wall, E. deBoer +1 more
The minimal enhancer fragment present in the 3'-flanking region of the human beta-globin gene contains four regions that bind nuclear proteins in vitro which are named NF-E1 and each of these regions interacts with at least one other protein factor.
Genes & DevelopmentFunctional analysis and in vivo footprinting implicate the erythroid transcription factor GATA-1 as a positive regulator of its own promoter.
332 Citations1991S F Tsai, Erich C. Strauss +1 more
It is proposed that a positive feedback loop mediated through GATA-1 serves two complementary functions: maintenance of the differentiated state by locking the promoter into an "on" state, and programming the progressive increase in protein content throughout cellular maturation.
Nucleic Acids ResearchTwo tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene
314 Citations1989Vincent Mignotte, Lee Wall +3 more
The erythroid-specific promoter of the human gene coding for Porphobilinogen Deaminase (PBGD) is studied by DNaseI footprinting, gel retardation and methylation interference assays and it is shown that this promoter contains three binding sites for the erythyroid- Specific factor NF-E1 and one site for a second erystroid- specific factor, which is named NF- E2.
CellThe HeLa cell protein TEF-1 binds specifically and cooperatively to two SV40 enhancer motifs of unrelated sequence
307 Citations1988Irwin Davidson, Jia Hao Xiao +3 more
A protein (TEF-1) is purified that specifically binds to two sequence unrelated motifs of the simian virus 40 (SV40) enhancer and this cooperative binding correlates with the ability of the tandem repeats to generate enhancer activity in vivo.
NatureIncreased γ-globin expression in a nondeletion HPFH mediated by an erythroid-specif ic DNA-binding factor
284 Citations1989David I. K. Martin, Shih‐Feng Tsai +1 more
The form of HPFH studied here is an inherited disorder which can be ascribed to the action of a cell-specific DNA-binding factor on a mutant promoter and is mediated by GF-1, the human counterpart of the chicken erythroid factor11 Eryf 1.
The EMBO JournalThe human beta‐globin promoter; nuclear protein factors and erythroid specific induction of transcription.
208 Citations1988E. deBoer, Michael Antoniou +3 more
The promoter of the human beta‐globin gene contains three regions in addition to the known CAC, CAAT and TATA box regions that are important for the induction of transcription in erythroid cells that bind the erythyroid specific nuclear factor NF‐E1 and a ubiquitous CAAT‐box factor.
NatureStructure and evolution of a human erythroid transcription factor
160 Citations1990Cecelia D. Trainor, Todd Evans +2 more
The cloning of the cDNA for the human Eryf 1 encoding gene, containing two 'finger' motifs, is almost identical to that of chicken or mouse, and indicates that these erythroid regulatory factors evolved from a common precursor composed of two distinct kinds of repeated domains, which subsequently evolved at greatly different rates.
The EMBO JournalThe −117 mutation in Greek HPFH affects the binding of three nuclear factors to the CCAAT region of the gamma‐globin gene.
143 Citations1988Giulio Superti‐Furga, Alcide Barberis +2 more
The Greek form of hereditary persistence of fetal hemoglobin (HPFH) is associated with a point mutation immediately upstream of the distal of the two CCAAT elements of the A gamma‐globin gene, suggesting a possible role of NF‐E in the repression of gamma-globin genes in adult erythroid cells.
Proceedings of the National Academy of SciencesStructure and promoter activity of the gene for the erythroid transcription factor GATA-1.
131 Citations1991Robert Hannon, Todd Evans +2 more
It is found that the upstream region of the gene functions as a powerful promoter when transfected into embryonic erythroid cells and suggests that GATA-1 protein plays an autoregulatory role in its own expression.
Genes & DevelopmentIn vitro binding of several cell-specific and ubiquitous nuclear proteins to the GT-I motif of the SV40 enhancer.
127 Citations1987J.H. Xiao, Irwin Davidson +5 more
The good correlation between the known cell-specific in vivo activity of the wild-type and mutated GT-I motif and the cell- specific binding of protein GT-IC in vitro strongly supports the conclusion that this protein is an enhancer factor.
Nucleic Acids ResearchThe effects of HPFH mutations in the human γ-globin promoter on binding of ubiquitous and erythroid specific nuclear factors
91 Citations1988Roberto Mantovani, Nicoletta Malgaretti +4 more
It is shown that single point mutations in the gamma-globin promoter may be the cause of high expression of the mutated gene in the adult period (Hereditary Persistence of Fetal Hemoglobin, HPFH), and one of these mutations characterized by a T----C substitution at position -175 in a conserved octamer sequence, abolishes the ability of a ubiquitous octamer binding nuclear protein to bind a gamma- globin promoter fragment containing the mutated sequence.
Nucleic Acids ResearchThe deletion of the distal CCAAT box region of the Aγ-globin gene in Mack HPFH abolishes the binding of the erythroki specific protein NFE3 and of the CCAAT displacement protein
65 Citations1989Roberto Mantovani, Giulio Superti‐Furga +2 more
It is reported that a 13 nucleotides deletion in the CCAAT box region of the A gamma-globin promoter, associated with greater than 100 fold overexpression of the gene, abolishes the in vitro binding of the ubiquitous factors CP1 and CDP and of the erythroid specific protein NFE3.
Nucleic Acids ResearchProgressive inactivation of the expression of an erythroid transcriptional factor in GM- and G-CSF-dependent myeloid cell lines
60 Citations1990Stefania Crotta, Silvia K. Nicolis +6 more
The results demonstrate that the specificity of the expression of NFE-1 for the erythroid--megakaryocytic--mast cell lineages is obtained by progressive inactivation of its expression in alternative lineages.
Nucleic Acids ResearchIncreased Sp1 binding mediates erythroid-specific overexpression of a mutated (HPFH) γ-globulin promoter
56 Citations1989Antonella Ronchi, Silvia K. Nicolis +2 more
It is shown that the -198 T----C mutation in the promoter of the A gamma-globin gene increases 20-30 fold the expression of this gene in adult erythroid cells of patients and creates a strong binding site, resembling a CACCC box, for two ubiquitous nuclear proteins, one of which is Sp1.
Nucleic Acids ResearchA protein factor binding to an octamer motif in the γ-globin promoter disappears upon induction of differentiation and hemoglobin synthesis in K562 cells
56 Citations1987Roberto Mantovani, Nicoletta Malgaretti +5 more
Using the electrophoretic mobility shift assay and the footprinting technique, the binding of nuclear proteins from erythroid and non erythyroid human cells to the promoter region of the human gamma-globin gene is studied and the possible role of these nuclear proteins in gamma- globin gene regulation and/or cell differentiation is discussed.
Nucleic Acids ResearchIncreased erythrold-specific expression of a mutated HPFH γ-globin promoter requires the erythroid factor NFE-1
41 Citations1989Silvia K. Nicolis, Antonella Ronchi +4 more
The present functional data indicate that altered binding of NFE1, but not of OTF1, is responsible for the observed overexpression of the mutated promoter.
Biochemical JournalDetection in non-erythroid cells of a factor with the binding characteristics of the erythroid cell transcription factor EF1
3 Citations1990Neil D. Perkins, Kim Orchard +3 more
This is the first report of an EF1-like activity in non-erythroid cells and indicates that this factor may play a role in the regulation of genes expressed in such cells.