Cloning and regulation of the vertebrate homologue of <i>lin</i>‐<i>41</i> that functions as a heterochronic gene in <i>Caenorhabditis elegans</i>
Developmental DynamicsPublished 13 February 2006
Takashi Kanamoto, Koji Terada, Hideki Yoshikawa, Takahisa Furukawa
Citations39
SJR quartileQ2
SJR score0.86
SNIP0.76
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TL;DR
The isolation of chick and mouse homologues of lin‐41 that encode the RBCC‐NHL family protein and their expression patterns are reported and it is shown that lin‐ 41 expression can be regulated through let‐7 complementary sites.
Abstract
The Caenorhabditis elegans gene lin-41 is the one of the heterochronic genes that regulate the timing of many developmental events. MicroRNA let-7 negatively regulates the expression of lin-41 through RNA-RNA interaction on its 3' untranslated region (UTR). Here, we report the isolation of chick and mouse homologues of lin-41 that encode the RBCC-NHL family protein and their expression patterns. C. elegans lin-41 is one of the RBCC-NHL families and the predicted amino acid sequences of isolated two genes encode the same family proteins. Chick and mouse lin-41 expression was also observed in developing limb buds, branchial arches, and tail buds. The 3'UTRs of the mouse and chick lin-41 genes contain multiple let-7 complementary sites. Using luciferase assay, we showed that lin-41 expression can be regulated through let-7 complementary sites.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
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