Initiation of protein synthesis in a rabbit reticulocyte lysate system
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein SynthesisPublished 1 January 1971
Wolfram Hoerz, Kenneth S. McCarty
Citations74
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TL;DR
Subunits are shown to be directly incorporated into polysomes during initiation of protein synthesis without evidence for the prior formation of a monosome.
Abstract
Initiation of protein synthesis in a cell-free system from rabbit reticulocytes has been resolved into two discrete steps by the use of the inhibitors NaF and aurintricarboxylic acid (ATA): (1) the attachment of a 40-S ribosomal subunit to polysomes which takes place in the presence of NaF but is blocked by ATA. (2) the addition of the 60-S ribosomal subunit in the formation of a complete ribosome that is prevented by NaF. Subunits are shown to be directly incorporated into polysomes during initiation of protein synthesis without evidence for the prior formation of a monosome.
Keywords
Biochemistry, Genetics and Molecular Biology
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