Abnormalities of Immunoregulatory T Cells in Disorders of Immune Function
New England Journal of MedicinePublished 8 November 1979
Ellis L. Reinherz, Arye Rubinstein, Raif S. Geha, Anthony J. Strelkauskas, Fred S. Rosen, Stuart F. Schlossman
Citations213
SJR quartileQ1
SJR score19.08
SNIP13.47
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
It is concluded that the characterization of T cells may provide insight into the causes of a number of abnormal immune states in man.
Abstract
We studied a five-year-old girl with several autoimmune disorders and a 16-year-old boy with acquired agammaglobulinemia to determine whether aberrations of immunoregulatory T cells could explain some instances of immunodeficiency or autoimmunity. The normal peripheral blood T-cell population, as defined by specific heteroantiserums, is 20 per cent TH2+ and 80 per cent TH2-. Human suppressor cells are TH2+, whereas helper cells are TH2-. In addition, each subset expresses Ia antigens upon activation. Our patient with autoimmune disease had no demonstrable TH2+ cells, and her lymphocytes could not be induced to suppress. Her circulating T cells were of an activated-helper phenotype, i.e., TH2-,Ia+. In contrast, in the boy with agammaglobulinemia, the T-cell population was predominantly of an activated-suppressor phenotype, i.e., TH2+,Ia+. This patient's T cells abrogated both his own and his histoidentical brother's B-cell secretion of immunoglobulins. We conclude that the characterization of T cells may provide insight into the causes of a number of abnormal immune states in man.
Keywords
Immunology and Microbiology
The LancetROLE OF SUPPRESSOR T CELLS IN PATHOGENESIS OF COMMON VARIABLE HYPOGAMMAGLOBULINÆMIA
711 Citations1974Thomas A. Waldmann, S Broder +4 more
Theses studies suggest that in some patients the disease common variable hypogammaglobulinaemia may be caused or perpetuated by an abnormality of regulatory T cells which act to suppress B-cell maturation and antibody production.
Journal of Clinical InvestigationSuppressor function of peripheral blood mononuclear cells in normal individuals and in patients with systemic lupus erythematosus.
436 Citations1977Barry Bresnihan, Hugo E. Jasin
Normal peripheral blood mononuclear cells demonstrated increased DNA synthesis and secretion of newly synthesized protein when suboptimal concentrations of Concanavalin A (Con A) were added to the cultures after 24-h incubation in vitro, suggesting that suppressor function in SLE returns as disease activity declines.
The Journal of ImmunologySubpopulations of Human T Cells Identified by Receptors for Immunoglobulins and Mitogen Responsiveness
428 Citations1976Lorenzo Moretta, Manlio Ferrarini +3 more
Although the two subpopulations show similar response patterns to concanavalin A, Tmu and Tgamma cells exhibit different dose-response curves to phytohemagglutinin, suggesting that synergistic interactions may occur.
Proceedings of the National Academy of SciencesDistribution of Ia-like molecules on the surface of normal and leukemic human cells.
321 Citations1976S F Schlossman, Leonard Chess +2 more
Antiserum to a glycoprotein antigen complex of 23,000 and 30,000 dalton subunits (p23,30), isolated and purified from a human lymphoblastoid B cell line, was shown to be highly specific for human bursal-equivalent-processed (B) cells, reactive with 15-20% of human Null cells, but completely unreactive with human thymus- Processed (T) cells.
PubMedImmunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens.
295 Citations1974Leonard Chess, Richard P. MacDermott +1 more
A Sephadex G-200 immunoabsorbent column bound with pure rabbit antihuman Fab was used to fractionate human peripheral lymphocytes and two distinct populations of cells were quantitatively recovered.
New England Journal of MedicineAberrations of Suppressor T Cells in Human Graft-versus-Host Disease
285 Citations1979Ellis L. Reinherz, Robertson Parkman +3 more
Analysis of T cells in patients with acute and chronic graft-versus-host disease suggested that the immunoregulatory cells may profoundly affect the overall immune response, and studies showing that these TH2+, la+ cells actively suppressed the in vitro immune response support this hypothesis.
The Journal of ImmunologyTechnical Aspects of the Rosette Tests Used to Detect Human Complement Receptor (B) and Sheep Erythrocyte-Binding (T) Lymphocytes
269 Citations1973Nelson F. Mendes, Marina E. A. Tolnai +3 more
The use of rosette formation and gradient centrifugation is demonstrated for the specific depletion of B or T cells from peripheral blood lymphocytes for complement receptor lymphocytes depletion.
Journal of Clinical InvestigationSuppression of B-cell differentiation by leukocytes from hypogammaglobulinemic patients.
223 Citations1976Frederick P. Siegal, Marta Siegal +1 more
Leukocytes from patients with several forms of immunodeficiency characterized by apparently differing defects in B-lymphocyte maturation produced few or no plasmacytoid cells in vitro, and were capable of suppressing the generation of plasma cells in co-culture with cells of normal persons, in the presence of pokeweed mitogen.
The Journal of ImmunologyTwo Functionally Distinct Subpopulations of Human T Cells That Collaborate in the Generation of Cytotoxic Cells Responsible for Cell-Mediated Lympholysis
203 Citations1978Robert L. Evans, Herbert Lazarus +2 more
Isolation of strongly reactive, TH2+, from weakly reactive,TH2- T cells by fluorescence-activated cell sorting revealed that the TH2+ subset contained most of the killer activity in cell-mediated lympholysis (CML), but had a diminished response in MLC and a suboptimal or negligible proliferative response to soluble antigens (mumps, PPD, tetanus toxoid).
New England Journal of MedicineHeterogeneity of “Acquired” or Common Variable Agammaglobulinemia
203 Citations1974Raif S. Geha, Eveline E. Schneeberger +2 more
Circulating T (thymus- derived) and B (bone-marrow-derived) lymphocytes were assessed in patients with common variable or "acquired" agamma-globulinemia and T-cell number and function were determined.
The Journal of ImmunologyImmunologic Functions of Isolated Human Lymphocyte Subpopulations
200 Citations1974Leonard Chess, Richard P. MacDermott +1 more
Proceedings of the National Academy of SciencesDirect evidence for loss of human suppressor cells during active autoimmune disease.
171 Citations1978A J Strelkauskas, Richard T. Callery +3 more
The studies indicate that a regulatory subset of lymphocytes is missing in patients with juvenile rheumatoid arthritis but these patients have antibodies in their serum that react with normal T cells, and the indication is the indication that serum from patients with autoimmune diseases may give a readily available reagent with which to dissect further functionally distinct subsets ofnormal T cells in man.
The Journal of Experimental MedicineDetection, isolation, and functional characterization of two human T-cell subclasses bearing unique differentiation antigens.
151 Citations1977Robert L. Evans, J Bréard +3 more
Functional analysis of the unreactive (TH1-) and reactive T-cell subclasses demonstrated that TH1- cells mounted a good proliferative response to a battery of specific soluble antigens but neither responded in MLC, nor elaborated LMF in response to tetanus toxoid.
The Journal of ImmunologyCon A-Inducible Suppression of MLC: Evidence for Mediation by the TH2+ T Cell Subset in Man
141 Citations1979Ellis L. Reinherz, Stuart F. Schlossman
The notion that the Con A-induced suppressor cell is confined to a distinct T cell subset in man and that T-T interactions are important in the overall expression of the immune response is supported.
The Journal of ImmunologyIsolation and Characterization of Naturally Occurring Subclasses of Human Peripheral Blood T Cells with Regulatory Functions
133 Citations1978Anthony J. Strelkauskas, Victoria Schauf +3 more
By utilizing naturally occurring autoimmune antibodies from patients with juvenile rheumatoid arthritis, the isolated and functionally characterized two unique subpopulations of T cells that respond very well to allogeneic cells, proliferate well in response to PHA but not Con A, and can greatly enhance the secretion of Ig by B cells.
New England Journal of MedicinePlasma Protein Measurements as a Diagnostic Aid
94 Citations1974Chester A. Alper
The plasma proteins function as enzymes, proteinase inhibitors, antibodies, clotting factors, complement components, kinin precursors and transport substances for hormones, vitamins, metals, lipids and other substances necessary for normal body function.
The Journal of ImmunologyPhenotypic and Functional Distinctions between the TH2+ and JRA+ T Cell Subsets in Man
58 Citations1979Ellis L. Reinherz, Anthony J. Strelkauskas +2 more
It is concluded that the TH2+ and JRA+ T cell subsets define distinct and different T cell populations in man.
The Journal of Experimental MedicineT-cell regulation of human peripheral blood B-cells responsiveness.
41 Citations1977A J Strelkauskas, Barry S. Wilson +3 more
It is shown that the B-cell population alone contains fewer secreting cells than the total lymphocyte population, and that T cells are required to achieve maximal plaque-forming cell levels.