Autologous limbal transplantation in patients with unilateral corneal stem cell deficiency
British Journal of OphthalmologyPublished 1 March 2000Open access
Harminder S. Dua
Citations196
SJR quartileQ1
SJR score1.81
SNIP1.83
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Autologous limbal transplantation with corneal, limbal, and conjunctival carriers was found to be useful for ocular surface reconstruction, over a mid-term follow up, in patients with unilateral stem cell deficiency.
Abstract
Autologous limbal transplantation with corneal, limbal, and conjunctival carriers was found to be useful for ocular surface reconstruction, over a mid-term follow up, in patients with unilateral stem cell deficiency. Close monitoring of the migration of conjunctival epithelium in the immediate postoperative period, and preventing it from crossing the limbus, ensured that the corneal surface was re-epithelialised exclusively from epithelial cells derived from the transplanted limbal tissue. This approach should improve the success of this procedure.
Keywords
Medicine
OphthalmologyLimbal Autograft Transplantation for Ocular Surface Disorders
1,083 Citations1989Kenneth R. Keivyon, Scheffer C.G. Tseng
Clinical results in 21 patients with follow-up of 6 months or more have consistently shown improved visual acuity, rapid surface healing, stable epithelial adhesion without recurrent erosion or persistent epithelial defect, arrest or regression of corneal neovascularization, and probable increased success for lamellar or penetrating keratoplasty.
CorneaHuman Allograft Limbal Transplantation for Corneal Surface Reconstruction
381 Citations1994Ray Jui-Fang Tsai, Scheffer C.G. Tseng
Preliminary results indicate that limbal allograft transplantation may be able to reconstruct a corneal surface that has undergone bilateral diffuse destruction, including the loss of limbal epithelial stem cells.
PubMedCorneal epithelial wound healing in the absence of limbal epithelium.
371 Citations1991Andrew Huang, S. C. G. Tseng
It is shown that only limited proliferative capacity of corneal epithelium remains in the absence of limbus, and the constellation of delayed healing with recurrent erosion, corNEal vascularization, and conjunctival epithelial ingrowth can be considered possible signs of limbal stem cell dysfunction.
British Journal of OphthalmologyTransplantation of amniotic membrane and limbal autograft for patients with recurrent pterygium associated with symblepharon
342 Citations1998J. Shimazaki, N Shinozaki +1 more
Transplantation of human amniotic membrane with a limbal autograft appears to be a promising surgical treatment for reconstructing the ocular surface in patients with recurrent pterygium associated with symblepharon.
PubMedCorneal epithelial wound healing in partial limbal deficiency.
277 Citations1990J. J. Y. Chen, S. C. G. Tseng
A deficiency of limbal stem cells contributes to the triad of conjunctival epithelial ingrowth, corneal vascularization, and delayed healing with recurrent erosion in rabbit corneas with partial limbal deficiency.
British Journal of OphthalmologyCorneal epithelial wound healing.
267 Citations1994Harminder S. Dua, José Álvaro Pereira Gomes +1 more
The corneal surface is, without question, the most specialised 123 mm2* of the body's surface and forms an integral part of the ocular surface with which it shares several anatomical and physiological attributes.
PubMedIn vitro propagation of human ocular surface epithelial cells for transplantation.
255 Citations1993Kristina Lindberg, Mary-Michael Brown +3 more
Results support the concept that corneal epithelial stem cells are located in the limbus and indicate that cultured autologous limbal cells may function as grafts to permanently restore the cornean epithelium after severe ocular surface injury.
OphthalmologyReconstruction of the Corneal Epithelium by Limbal Allograft Transplantation for Severe Ocular Surface Disorders
254 Citations1995Kazuo Tsubota, Ikuko Toda +3 more
Limbal allograft transplantation with intensive immunosuppression by cyclosporine and high-dose steroids appears to be a promising surgical intervention for the reconstruction of corneas affected by severe ocular surface disease.
PubMedAbnormal corneal epithelial wound healing in partial-thickness removal of limbal epithelium.
244 Citations1991J J Chen, S. C. G. Tseng
It is evident that in vivo n-heptanol treatment for different durations can result in different extents of corneal conjunctivalization and vascularization, and the capability of the remaining limbal basal epithelium to recover its original full-thickness stratified layers determines the strength of the limbal barrier.
American Journal of OphthalmologyThe Corneoscleral Limbus in Human Corneal Epithelial Wound Healing
230 Citations1990Harminder S. Dua, John V. Forrester
During re-epithelialization of the ocular surface in 17 human eyes with large corneal and conjunctival abrasions, cell movement was found to occur circumferentially along the corneoscleral limbus and centripetally from the cornea, completing the healing process of the limbal region.
American Journal of OphthalmologyPostoperative Instillation of Low-Dose Mitomycin C in the Treatment of Primary Pterygium
202 Citations1988Seiji Hayasaka, Sachiko Noda +2 more
The postoperative instillation of 0.02% mitomycin C, twice a day for five days, is found to be effective and safe in the treatment of primary pterygium.
EyeLimbal transplantation in the management of chronic contact-lens-associated epitheliopathy
192 Citations1993Christopher W. Jenkins, Stephen J. Tuft +2 more
It is concluded that limbal stem cell dysfunction in chronic contact-lens-associated epitheliopathy may be subclinical and that autograft transplantation in bilaterally exposed patients may fail to restore the epithelial phenotype of the host eye whilst jeopardising the epithel integrity of the donor eye by depleting its stem cell reserve.
OphthalmologyLimbal Transplantation
188 Citations1996Donald Tan, Linda Ficker +1 more
Although it requires a longer follow-up period, limbal allografted transplantation is a viable procedure for bilateral ocular surface disease and for presumed bilateral disease and may be a preferred alternative to limbal autograft transplantation for such patients.
British Journal of OphthalmologyThe conjunctiva in corneal epithelial wound healing
186 Citations1998Harminder S. Dua
There is little clinical evidence to support the concept that conjunctival transdifferentiation per se, occurs in humans, but in patients with partial stem cell deficiency this approach can be a useful and effective alternative to partial limbal transplantation, as is currently practised.
Archives of OphthalmologyConjunctival Transplantation
181 Citations1977R A Thoft
In three of five eyes with total corneal involvement, successful reepithelialization of the cornea from the conjunctiva was accompanied by improved vision and decreased vascularization, and results in two other eyes were less successful due to deep stromal vascularization.
EyeStem cells and corneal epithelial regeneration
158 Citations1994Friedrich E. Kruse
Clinically, several ocular surface disorders such as chemical burns can cause limbal damage and consecutive limbal insufficiency and treatment for these disorders is available only by transplantation of healthy stem cells, which can be performed as both autograft and allograft.
British Journal of OphthalmologyAllo-limbal transplantation in patients with limbal stem cell deficiency
150 Citations1999Harminder S. Dua, Augusto Azuara‐Blanco
Allo-limbal transplantation, with systemic immunosuppression with FK-506 is useful in reconstruction of the ocular surface with improvement in vision in patients with severe stem cell deficiency.
Archives of OphthalmologyImpression Cytology Study of Epithelial Phenotype of Ocular Surface Reconstructed by Preserved Human Amniotic Membrane
147 Citations1997Pinnita Prabhasawat
The lack of corneal epithelial phenotype even on an avascular corneAL stroma supports the concept that conjunctival transdifferentiation does not occur in vivo, and indicates that additional limbal stem cell transplantation is needed for effective cornean surface reconstruction in patients with limbal deficiency.
PubMedLimbal epithelium in ocular surface wound healing.
119 Citations1982S. Kinoshita, Timothy C. Kiorpes +2 more
It is proposed that there are three distinct types of ocular surface epithelia--corneal, bulbar conjunctival, and limbal--and that limbal epithelium behaves differently from corneal and conjunctive epithelio in ocularsurface wound healing.
OphthalmologyPrimary Intraocular Lens Implantation in the Setting of Penetrating Ocular Trauma
103 Citations1995Patrick E. Rubsamen, W. David Irvine +3 more
Intraocular lens implantation at the time of lensectomy and primary repair of a corneal laceration allows good visual rehabilitation with restoration of binocular function and serves as an alternative to contact lens correction in select patients.
British Journal of OphthalmologyLong term results of intraoperative mitomycin C in the treatment of recurrent pterygium.
100 Citations1996Leonardo Mastropasqua, Paolo Carpineto +2 more
This study confirms the efficacy of intraoperative mitomycin C in improving the success rate after recurrent pterygium surgical excision.
EyeLimbal autotransplantation in the acute and chronic phases of severe chemical injuries
97 Citations1996Stephen J. Morgan, Aidan Murray
In five of the six cases of severe chemical burn of the eye treated by limbal autotransplantation, the stability of the ocular surface was markedly improved, resulting in two of the three acute cases in full recovery of normal vision and in the three chronic cases in improved comfort and reduced corneal opacity and vascularity.
OphthalmologyReconstruction of the Periocular Mucous Membrane by Autologous Conjunctival Transplantation
84 Citations1982David W. Vastine, William Stewart +1 more
The concept of autologous conjunctival transplantation for corneal resurfacing as recommended by Thoft is extended to reconstruction in 14 patients with unilateral abnormalities of the bulbar and palpebral conjunctiva caused by alkali burns, irradiation, neoplasms, degenerative diseases, trauma, and developmental anomalies.
American Journal of OphthalmologyLimbal Autograft Reconstruction After Conjunctival Squamous Cell Carcinoma
71 Citations1990Robert A. Copeland, Devron H. Char
Autologous limbal transplants were effective in restoring an excellent corneal surface and good visual function in two patients who had squamous cell carcinoma with extensive limbal and cornea involvement.
Archives of OphthalmologyIndications for Keratoepithelioplasty
67 Citations1990Paul W. Turgeon
The results suggest that epithelial transplantation may be a useful option in the care of chronic ocular surface failure unresponsive to conventional medical management.
OphthalmologyTreatment of Contact Lens-related Ocular Surface Disorders with Autologous Conjunctival Transplantation
55 Citations1992Thomas Clinch, Kenneth M. Goins +1 more
Tissue culture demonstrated a decreased replication rate in the limbal epithelial cells, which suggests that epithelial stem cell dysfunction may be responsible for this ocular surface disease.
OphthalmologyConjunctival Autograft Transplantation for Unilateral Ocular Surface Diseases
39 Citations1983Wesley K. Herman, Donald J. Doughman +1 more
A new surgical technique for diseased ocular surface preparation and for the acquisition, transfer, and placement of a 360 degrees ring of autologous conjunctiva in patients with unilateral surface pathology is introduced.
Experimental Eye ResearchBiochemical transformation of bulbar conjunctiva into corneal epithelium: An electrophoretic analysis
33 Citations1985Thomas M. Harris, Edward R. Berry +2 more
Electrophoretic patterns of soluble proteins from regenerating epithelium suggest that the regeneration process occurs in three distinct phases: 24-72 hr post-scraping is a period of de-differentiation, 72 hr to 1 week is a Period of reorganization, and 1-6 weeks is aperiod of differentiation.
International Ophthalmology ClinicsReconstruction of Corneal Epithelium by Limbal Allograft Transplantation for Severe Ocular Surface Disorders
20 Citations1995Kazuo Tsubota, Ikuko Toda +3 more
International Ophthalmology ClinicsReconstruction of Corneal Epithelium by Limbal Allograft Transplantation for Severe Ocular Surface Disorders
15 Citations1995Kazuo Tsubota, Ikuko Toda +3 more