Strand breakage in DNA by silicic acid
Mutation Research LettersPublished 1 May 1988
Sikandar G. Khan, R. Y. Rizvi, S.M. Hadi, Qamar Rahman
Citations9
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The alkaline unwinding assay has been used to demonstrate the formation of single-strand breaks in DNA on treatment with silicic acid, and the possibility of creation of apurinic or apyrimidinic sites is suggested.
Abstract
The alkaline unwinding assay has been used to demonstrate the formation of single-strand breaks in DNA on treatment with silicic acid. Double-stranded DNA, containing no single-strand breaks, when incubated with increasing concentrations of silicic acid, showed the formation of an increasing number of strand breaks per molecule. Experiments on reduction of silicic acid-treated DNA with NaBH4 suggested the possibility of creation of apurinic or apyrimidinic sites. The significance of silicic acid interaction with cellular DNA during asbestos exposure is discussed.
Keywords
Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology
Biochemical JournalA study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid
14,126 Citations1956K. Burton
The present study arose from the observation that a more intense colour was sometimes produced if, instead of being heated at 1000 for 10 min., the reaction mixture was allowed to stand overnight at room temperature.
Medical Entomology and ZoologyThe Chemistry of Silica: Solubility, Polymerization, Colloid and Surface Properties and Biochemistry of Silica
2,395 Citations1979R. K. Iler
The AnalystThe colorimetric determination of silicon in the micro-analysis of biological material and mineral dusts
108 Citations1955Edward J. King, B. D. Stacy +3 more
Analytical BiochemistryQuantitation of chemically induced DNA strand breaks in human cells via an alkaline unwinding assay
87 Citations1985Florian Daniel, Dorothee Haas +1 more
A correlation was shown between mammalian DNA strand-breaking potential (as measured in this procedure) and the propensity of these chemicals to revert Salmonella typhimurium TA100.
Mutation Research/Genetic ToxicologyThe genetic effects of crocidolite asbestos; comparison of chromosome abnormalities and sister-chromatid exchanges
73 Citations1980Molly Jean Price-Jones, Gillian Gubbings +1 more
The genetic effects of crocidolite asbestos in Chinese hamster V79-4 cells are compared with those of potassium chromate and Min-u-Sil silica to show whether these substances induced SCE or induced numerical changes in the karyotype.
Analytical BiochemistryA hydroxylapatite batch assay for quantitation of cellular DNA damage
69 Citations1979Peter M. Kanter, Herbert S. Schwartz
A batch elution procedure is described for quantitative measurement of DNA damage, which is rapid, permits large numbers of samples to be handled simultaneously, and consistently yields recoveries of >95% of total chromatographed DNA.
Environmental ResearchCytogenetic studies on chrysotile asbestos
63 Citations1975K. S. Lavappa, Maoyong Fu +1 more
Oral or intraperitoneal administration of chrysotile induced a significant and dose-related increase in chromosome aberrations and a dose- related inhibition of the mitotic index of cultured Syrian hamster embryo cells.
International Journal of CancerCellular ingestion, toxic effects, and lesions observed in human bronchial epithelial tissue and cells cultured with asbestos and glass fibers
39 Citations1982Aage Haugen, Paul W. Schafer +4 more
The preferential cytotoxicity of asbestos for epithelial cells and the metaplastic response of bronchial epithelium may be important in the cocarcinogenic action of asbestos.
Environmental MutagenesisRapid analysis of DNA strand breaks in soft tissues
34 Citations1985Stanton R. Morris, Howard G. Shertzer
Using the described technique, a dose-effect relationship was observed between N-nitrosodimethylamine (NDMA) and alkaline unwinding of mouse liver DNA.
Environmental Health PerspectivesEffects of ingested chrysotile on DNA synthesis in the gastrointestinal tract and liver of the rat
32 Citations1974David E. Amacher, Adhid Al-Arif +1 more
The data suggest that asbestos penetrates the gastrointestinal mucosa and influences regulation of DNA synthesis in the gastrointestinal tract of the rat and is responsible for a transient increase in DNA synthesis.
BiochemistryIntermediates in excision repair by human cells: use of S<sub>1</sub> nuclease and benzoylated naphthoylated cellulose to reveal single-strand breaks
28 Citations1977Peter Karran, N. Patrick Higgins +1 more
It is concluded that MMS- and AAAF-induced damage are repaired by different pathways in human cells and that DNA with discontinuities is a readily detectable intermediate only in the repair of MMS damage by the apurinic pathway.
Archives of Environmental Health An International JournalCytodynamic Reactivity of the Mesothelium
26 Citations1971Samuel Bryks, Felix D. Bertalanffy
Observations indicate that the pleural mesothelium plays a high degree of sensitivity to chrysotile asbestos, even when administered by the intratracheal route.
Cold Spring Harbor Symposia on Quantitative BiologyEndonuclease II of E. coli
23 Citations1968E C Friedberg, David A. Goldthwait
Environmental Health PerspectivesEffect of asbestos on the P388D1 macrophagelike cell line: preliminary ultrastructural observations.
12 Citations1983Neil F. Johnson, R. Davies
It was concluded that the cytotoxic effect of asbestos towards the cells was due to mechanical effects, e.g., by penetrating structures such as the nucleus or phagosomes, by preventing the ordered movement of organelles with the cell or by disrupting the cytoskeletal framework of the cell.
Mutation Research LettersDegradation of DNA by silicic acid
6 Citations1984Qamar Rahman, Nasir K. Alvi +2 more
Native calf thymus DNA was incubated with increasing concentrations of silicic acid and subjected to S1 nuclease hydrolysis, suggesting an increasing degree of DNA degradation was seen suggesting a destabilization of the secondary structure.