Chromosomal loci of Neurospora crassa
Microbiological ReviewsPublished 1 December 1982Open access
Dmitri Perkins, Alan Radford, Dorothy Newmeyer, Mari Björkman
Citations344
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Methods in enzymology on CD-ROM/Methods in enzymology[4] Genetic and microbiological research techniques for Neurospora crassa
1,117 Citations1970Rowland H. Davis, Frederick J. de Serres
This chapter describes the microbiological and genetic handling of Neurospora crassa and reviews the biochemical methods of neurospora, a member of the fungal class Ascomycetes.
The American NaturalistDistribution of Lysine Pathways Among Fungi: Evolutionary Implications
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The remarkable consistency of the distribution of the two lysine paths suggests that (a) they did not arise sporadically; (b) their distribution pattern was not disturbed by genetic exchange; and (c) there is a substantial evolutionary gap in organisms differing in path of l Lysine synthesis.
Advances in geneticsThe Cytogenetics of Neurospora
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Neurospora possesses several favorable features compared to the more conventional organisms that are used in cytogenetic research, and these, in part, compensate for the small size of its chromosomes.
Advances in geneticsMap Construction in Neurospora Crassa
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Journal of General MicrobiologyThymidine Kinase: Evidence for its Absence from Neurospora crassa and Some Other Micro-organisms, and the Relevance of This to the Specific Labelling of Deoxyribonucleic Acid
204 Citations1968Anthony R. Grivell, J. F. Jackson
Thymidine kinase (EC 2.7.1.21) was not detected in cell-free extracts of Neurospora crassa, Aspergillus nidulans, Saccharomyces cerevisiae or Euglena gracilis, and it is considered that the absence of thymidine Kinase prevents the specific incorporation of Thymidine into deoxyribonucleic acid in intact cells of neurospora Crassa.
PLANT PHYSIOLOGYPhotoregulation of the Carotenoid Biosynthetic Pathway in Albino and White Collar Mutants of <i>Neurospora crassa</i>
162 Citations1981Roy W. Harding, Richard V. Turner
The soluble enzyme fraction obtained from the albino-3 mutant was shown to be almost totally defective in enzyme activity required for the biosynthesis of [(14)C]geranylgeranyl pyrophosphate from [1-C]isopentenyl pyroph phosphate.
GeneticsSPORE KILLER, A CHROMOSOMAL FACTOR IN NEUROSPORA THAT KILLS MEIOTIC PRODUCTS NOT CONTAINING IT
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Three chromosomal factors called Spore killer (Sk) have been found in wild populations of Neurospora sitophila and N. intermedia, and are similar to other examples of meiotic drive such as Segregation Distorter in Drosophila, Pollen killer in wheat, and Gamete eliminator in tomato.
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136 Citations1981Eric U. Selker, Charles Yanofsky +4 more
The pattern of nucleotide substitutions between the predicted Neurospora 5S RNAs and between these and S. cerevisiae 5S RNA suggests that a particular 5SRNA secondary structure occurs in vivo and is conserved.
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Re recessive lethal mutations at specific loci induced in a heterokaryotic cell result from the same spectrum of genetic alterations that is inducing in a diploid cell.
Proceedings of the National Academy of SciencesMendelian and Non-Mendelian Factors Affecting the Cytochrome System in <i>Neurospora Crassa</i>
127 Citations1953M. B. Mitchell, Herschel K. Mitchell +1 more
Among 8795 ascospores isolated from irradiated material during their study by one of the present authors, 54 gave rise to strains having easily diagnosed requirements for components present in the complete medium used and, therefore, represented the class of nutritional mutants sought.
Archives of Biochemistry and BiophysicsBiosynthesis of carotenoids in Neurospora. Action spectrum of photoactivation
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The amount of carotenoids produced in Neurospora was proportional to the light dosage, and it was assumed that a flavin was the photoreceptor and no other pigments with a similar spectrum could be detected in neurospora.
Biochemical JournalControl of the flux in the arginine pathway of <i>Neurospora crassa</i>. Modulations of enzyme activity and concentration
116 Citations1981Harry J. Flint, R. W. Tateson +4 more
It is shown that control of the flux is shared among all the enzymes of the pathway, and the concept of a 'rate-limiting step' is found to be inadequate and is replaced by a quantitative measure, the Sensitivity Coefficient, which takes account of all the interactions.
Proceedings of the National Academy of SciencesIdentification and characterization of recombinant plasmids carrying the complete qa gene cluster from Neurospora crassa including the qa-1+ regulatory gene.
109 Citations1981Michael Schweizer, Mary E. Case +3 more
This communication describes a series of recombinant plasmids that span 36.5 kilobases of linkage group VII and contain the coding sequences for qa-2,qa-4, qo-3, and the qa -1 regulatory protein, which encodes a positive regulatory protein which, in the presence of quinic acid, leads to the de novo synthesis of the other proteins in theqa cluster.
Proceedings of the National Academy of SciencesA gene cluster in Nuerospora crassa coding for an aggregate of five aromatic synthetic enzymes.
109 Citations1967Norman H. Giles, Mary E. Case +2 more
The mutants studied are polyaromatic auxotrophs which typically require for growth tryptophan, phenyl-alanine, tyrosine, and para-aminobenzoic acid and have proved to be a considerable problem with many of the arom mutants.
ScienceMetabolite Distribution in Cells
98 Citations1972Rowland H. Davis
In "Metabolite distribution in cells," by R. H. Davis (24 November 1972), two errors occurred.
GeneticsNEW MARKERS AND MULTIPLE POINT LINKAGE DATA IN NEUROSPORA
93 Citations1959David D. Perkins
Improved and extended maps based on new data have been provided by augment and improve the maps in order to provide suitably marked chromosomes for investigations related to crossing over.
Advances in microbial physiology/Advances in Microbial PhysiologyNitrate Assimilation in Fungi
90 Citations1979Reginald H. Garrett, Nancy Klein Amy
The chapter analyzes the results available in the process of nitrate assimilation as it occurs in two filamentous ascomycetes— Aspergillus nidulans and Neurospora crassa, providing the most concise and comprehensive description of nitrates assimilation and its regulation.
Journal of BacteriologyNovel Mutation Causing Derepression of Several Enzymes of Sulfur Metabolism in <i>Neurospora crassa</i>
90 Citations1972Earl G. Burton, Robert L. Metzenberg
A group of enzymes of sulfur metabolism, normally repressed in Neurospora crassa by an abundant supply of a "favored" sulfur source, are isolated in which the formation of each of these enzymes is largely or completely nonrepressible.
Neurospora crassa: A Unique System for Studying Circadian Rhythms
85 Citations1983Jerry F. Feldman, Jay Dunlap
The value of E. coli to molecular biology, the mammalian red blood cell to membrane biochemistry, and the oat coleoptile to plant physiology has been well-documented.
Proceedings of the National Academy of SciencesControl of the synthesis of a single enzyme by multiple regulatory circuits in Neurospora crassa.
83 Citations1975Mark A. Hanson, George A. Marzluf
The evidence suggests that a single structural gene for this extracellular protease exists and that it is regulated in a complex fashion such that control signals arising from any one of the three distinct regulatory circuits can activate it for expression.
Journal of Biological ChemistryThe Regulation of Methionine Synthesis and the Nature of Cystathionine γ-Synthase in Neurospora
81 Citations1970Douglas S. Kerr, Martin Flavin
An ethionine-resistant mutant which over-produced methionine was found to be unable to accumulate the inhibitor due to a defect in S-adenosylmethionine synthase; this is believed to be the first identification of a gene coding this enzyme.
Journal of Molecular BiologyGenetically determined multiple forms of glutamic dehydrogenase in Neurospora crassa
78 Citations1962J. R. S. Fincham
The TPN†-linked glutamic dehydrogenase of Neurospora crassa has been obtained in approximately pure form and the pairs of mutants am 1 + am 2, am1 + am 3 and am 1 - am 12 show complementation ; that is to say, they can form glutamic dehydrationase of relatively normal properties in heterocaryons.
Journal of Biological ChemistryDerepression of Tyrosinase Synthesis in Neurospora by Cycloheximide, Actinomycin D, and Puromycin
78 Citations1970N. H. Horowitz, Helen Macleod Feldman +1 more
Results suggest that synthesis of the enzyme is controlled by an unstable repressor protein, and these antibiotics inhibit the synthesis de novo of tyrosinase in cultures of Neurospora crassa which have been derepressed by fasting.
GeneticsGENETICS OF A COLONIAL MICROCONIDIATING MUTANT STRAIN OF NEUROSPORA CRASSA
77 Citations1949R. W. Barratt, Laura Garnjobst
Genetical analysis of a mutant strain of AVeurospora crassa having these characteristics, the interaction of the genes concerned, and the development of derived strains show that the single gene, colonial-1 (col-1)) differentiates between a culture derived from U-8743 having colonial growth and one having spreading growth.
Journal of Biological ChemistryIndole Channeling by Tryptophan Synthase of Neurospora
75 Citations1974William H. Matchett
Results are interpreted as evidence that the Neurospora enzyme fits well the conceptual framework of the "surface model" and that the enzyme may have evolved, as first suggested, from a primitive multicomponent complex.
Journal of BacteriologyGenetic Determinants of Circadian Rhythmicity in <i>Neurospora</i>
75 Citations1969Malcolm Sargent, Dow O. Woodward
Timex, a strain of Neurospora crassa which exhibits a circadian rhythm of conidia formation in growth-tube cultures, has been found to differ from wild-type strains by two genes, one of which is responsible for an invertase deficiency, whereas the second gene is of unknown function.
Journal of BacteriologyMolecular Sieving by Neurospora Cell Walls During Secretion of Invertase Isozymes
74 Citations1966John R. Trevithick, Robert L. Metzenberg
The hypothesis is offered that the increased secretion of invertase and decreased degree of fractionation seen in osmotic mutants, and to a lesser extent in the other mutants, can be explained by an increased porosity of the cell wall.
Proceedings of the National Academy of SciencesDefective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa.
73 Citations1979Carmen A. Mannella, Richard A. Collins +2 more
Two temperature-sensitive nuclear mutants are identified that fail to excise the intervening sequence at the nonpermissive temperature (37 degrees C) and should provide powerful new tools for studying RNA processing in eukaryotic cells.
GeneticsLINKAGE STUDIES WITH BIOCHEMICAL MUTANTS OF NEUROSPORA CRASSA
71 Citations1949Mary B. Houlahan, G. W. Beadle +1 more
GeneticsGENETIC CONTROL OF NITRATE REDUCTASE IN <i>NEUROSPORA CRASSA </i>
71 Citations1965George J. Sorger, Norman H. Giles
At least one of the steps in the electron transport sequence from TPNH to nitrate in Neurospora nitrate reductase has been demonstrated to be inducible by nitrate and to remain physically associated the ascospores both inside and out of the perithecia.
Journal of Cellular and Comparative PhysiologyMutagenesis and chromosome structure
70 Citations1964Frederick J. de Serres
The spectra of forward-mutations occurring spontaneously or induced by X rays, nitrous acid, or 2-aminopurine at the ad-3A andad-3B loci of Neurospora crassa have been compared by means of tests for allelic complementation and leakiness and suggest that there is a correlation between the type of genetic alteration and thetype of complementation pattern.
GeneticsTHE USE OF DUPLICATION-GENERATING REARRANGEMENTS FOR STUDYING HETEROKARYON INCOMPATIBILITY GENES IN NEUROSPORA
70 Citations1975David D. Perkins
The results show how that the duplication method can be used to identify and map additional vegetative incompatibility loci, without the necessity of heterokaryon tests.
Archives of Biochemistry and BiophysicsA mutant form of ornithine transcarbamylase found in a strain of Neurospora carrying a pyrimidine-proline suppressor gene
69 Citations1962Rowland H. Davis
It has been found that the enzyme present in the s strain is different from the normal enzyme in its affinities for ornithine and carbamyl phosphate, and this characteristic could compensate to a large extent for the low specific activity in s strains.
Journal of BacteriologyBasic amino acids and inorganic polyphosphates in Neurospora crassa: independent regulation of vacuolar pools
68 Citations1980Carole L. Cramer, L E Vaughn +1 more
It is concluded that N. crassa, like yeasts, can regulate polyphosphates and basic amino acids independently, and that the accumulation ofbasic amino acids in vesicles may depend upon an energy-requiring mechanism in addition to the demonstrated charge interaction with polyph phosphate.
Archives of Biochemistry and BiophysicsA gene affecting the repression of invertase and trehalase in Neurospora
68 Citations1962Robert L. Metzenberg
Evidence is presented that the levels of enzymes are affected by a single gene alteration that produces much larger amounts of these two enzymes than does the parental strain.
Proceedings of the National Academy of SciencesThe regulation of synthesis of leucine biosynthetic enzymes in Neurospora.
67 Citations1965S R Gross
Synthetase activity was measured at 340 by the spectrophotometric procedure of Webster and Gross3 in a 1-ml reaction mixture containing 0.1 jsmole acetyl CoA, 0.mole N-ethylmaleimide, and0.1 mmole KPO4 buffer at pH 7.5 with 0.01 ml enzyme extract.
Journal of BacteriologyAdenosine 3',5'-cyclic monophosphate and morphology in Neurospora crassa: drug-induced alterations
65 Citations1975William Scott, Bruce Solomon
Evidence indicates a relationship between the degree of morphological abnormality, the degree to which intracellular cAMP is reduced, and the action of the drugs on the adenyl cyclase and phosphodiesterase.
Proceedings of the National Academy of SciencesCircadian rhythms in Neurospora crassa: a mutation affecting temperature compensation.
64 Citations1982Daniell L. Mattern, Linda Forman +1 more
Cel displayed a threshold temperature or "break point" for the temperature compensation of its rhythm, and Supplementation of cel strains, which require fatty acids, with unsaturated or short-chain fatty acids raised the threshold temperature to 26 degrees C, whereas supplementation with long-chain saturated fatty acids lowered it to 18 degrees C.
PROTOPLASMAEffects of cytochalasins onNeurospora crassa
64 Citations1980Edward D. Allen, R. Aiuto +1 more
Mutation Research/Fundamental and Molecular Mechanisms of MutagenesisBiochemical basis of radiation-sensitivity in mutants of Neurospora crassa
63 Citations1973Thomas E. Worthy, J.L. Epler
Tentative analogies were drawn between the Neurospora mutants and the known classes of UV-sensitive mutants in E. coli and relationships were seen between repair defects and mutation induction, although too little data was available for any definitive conclusions.
GeneticsULTRAVIOLET-SENSITIVE MUTANTS IN <i>NEUROSPORA CRASSA</i>
62 Citations1967Lung-Ting Chang, R. W. Tuveson
Journal of BacteriologyProperties of two cyclic nucleotide-deficient mutants of Neurospora crassa
59 Citations1979Gary B. Rosenberg, Martin L. Pall
The semicolonial morphology of another adenylate cyclase-deficient mutant of Neurospora, frost, was not corrected by exogenous cyclic nucleotides or by phosphodiesterase inhibitors indicating that the frost morphology is probably not caused by low endogenous cAMP levels.
Journal of BacteriologyAcetate-nonutilizing Mutants of <i>Neurospora crassa</i> I. Mutant Isolation, Complementation Studies, and Linkage Relationships
57 Citations1968R. B. Flavell, J. R. S. Fincham
Sixty mutants of Neurospora crassa unable to grow on acetate as sole source of carbon, but able to utilize sucrose, were isolated and six of the genes have been mapped; no two are closely linked.
Archives of Biochemistry and BiophysicsPhotochemical studies of the carotenoid biosynthetic pathway in Neurospora crassa
57 Citations1969Roy W. Harding, P.C. Huang +1 more
Labeling and time-course data are presented which support the proposal that the carotenoids are all synthesized from phytoene by a series of sequential reactions.
Proceedings of the National Academy of SciencesEvidence for a Tryptophane Cycle in Neurospora
55 Citations1949F. A. Haskins, Herschel K. Mitchell
It is indicated that anthranilic acid is a precursor to indole in Neurospora, which requires either tryptophane or indole for growth.
Canadian Journal of Genetics and CytologyNULL MUTANTS OF THE<i>A</i>AND<i>a</i>MATING TYPE ALLELES OF<i>NEUROSPORA CRASSA</i>
55 Citations1982Anthony Griffiths
Thirty-five null mutants have now been obtained of the A mating type allele, which have simultaneously lost both known functions-heterokaryon incompatibility and sexual compatibility with strains of EMT.
Proceedings of the National Academy of SciencesTHE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. ENZYMATIC TRANSFORMATION OF THE DIHYDROXY ACID PRECURSORS TO THE KETO ACID PRECURSORS
54 Citations1954John W. Myers, Edward A. Adelberg
It has been shown that the last 'two steps in the synthesis of these amino acids proceed through homologous compounds, namely, from the corresponding a,f-dihydroxy acids (DHI and DHV)1 through the a-keto acids (KI and KV).
Journal of Biological ChemistryThe subunit structure of tryptophan synthase from Neurospora crassa.
53 Citations1975WH Matchett, J A DeMoss
Results are interpreted as indicating that the Neurospora enzyme is a homodimer, after purifying to electrophoretic homogeneity from the wild type strain 74A.
Molecular and General Genetics MGGThe role of glutamine synthetase and glutamine metabolism in nitrogen metabolite repression, a regulatory phenomenon in the lower eukaryote Neurospora crassa
50 Citations1980Nigel Dunn-Coleman, Reginald H. Garrett
It is proposed that under conditions of NH4+sufficiency, and hence glutamine sufficiency, the octameric form of GS represses nit-2 gene expression and thereby achieves ammonium repression.
Canadian Journal of BotanyMeiotic nuclear behavior and ascospore formation in five homothallic species of <i>Neurospora</i>
50 Citations1978Namboori B. Raju
Ascus development, haploid chromosome numbers, and morphology in all five homothallic species resemble the heterothallic N. crassa, and the following observations have not been reported previously for any Neurospora species, although some have been described in other fungi.
Journal of Molecular BiologyMultiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro
50 Citations1966Alan Coddington, J. R. S. Fincham +1 more
The results are interpreted in terms of conformational correction of the potentially active am 19 protein subunits by hybridization with the conformationally normal, but inactive am 1 subunits.
GeneticsGENETIC AND PHYSIOLOGICAL CHARACTERISTICS OF A SLOW-GROWING CIRCADIAN CLOCK MUTANT OF <i>NEUROSPORA CRASSA</i>
49 Citations1978Jerry F. Feldman, Cheryl A Atkinson
A circadian clock mutant of Neurospora crassa with a period length of about 25.8 hours (4 hr longer than wild type) has been isolated after mutagenesis of the band strain, and it is recessive in heterokaryons and grows at about 60% the rate of the parent band strain on both minimal and complete media.
GeneticsALLELIC RECOMBINATION IN NEUROSPORA: TETRAD ANALYSIS OF A THREE-POINT CROSS WITHIN THE <i>pan-2</i> LOCUS
49 Citations1964Mary E. Case, Norman H. Giles
HE analysis of tetrads from crosses between allelic mutants at the pan-2 locus in Neurospora crassa has provided considerable information concern-ing allelic (intragenic) recombination mechanisms, but the evidence indicates that the great majority of nonreciprocal tetrades cannot be explained on the basis of orthodox although unusual chromosome behavior, such as heteroploidy.
Proceedings of the National Academy of SciencesTHE RELATIONSHIP OF GENE CONVERSION TO CROSSING OVER IN NEUROSPORA
49 Citations1959David Stadler
A hypothesis whereby a single event results in both gene conversion and crossing over was formulated by Freese and this scheme was designed to account for the apparent differences between the two types of recombination reported by Mitchell.
Biochemical JournalSlow conformational changes of a <i>Neurospora</i> glutamate dehydrogenase studied by protein fluorescence
48 Citations1974Barrie Ashby, John C. Wootton +1 more
The results show that the slow reversible conformational change detected by the change in fluorescence is the rate-limiting process for enzyme activation and inactivation, and these kinetics and published measurements of molecular weight are indicative of an isomerization process.
GeneticsA MUTANT AFFECTING MEIOSIS IN NEUROSPORA
48 Citations1975David A. Smith
The usefulness of the criterion white-ascospore-production as a signal for the presence of a mutant affecting meiosis is demonstrated by the recovery of several such mutants, including mei-1 (meiotic-1), a recessive mutant on linkage group IV.
Journal of BacteriologyNuclear division cycle in germinating conidia of Neurospora crassa
47 Citations1978Laura Serna, David Stadler
It is concluded that the nuclei in a population of dormant conidia are arrested at various points in the nuclear division cycle.
GeneticsAN INSERTIONAL TRANSLOCATION IN NEUROSPORA THAT GENERATES DUPLICATIONS HETEROZYGOUS FOR MATING TYPE
45 Citations1972David D. Perkins
The initial inhibition is attributed to mating-type heterozygosity, and escape to a somatic event that makes mating type homoor hemizygous, and genes that were initially heterozygous in the duplication segregate when escape occurs.
Journal of General MicrobiologyA Nuclear Gene Suppressor of a Cytoplasmically Inherited Character in Neurospora crassa
45 Citations1956M. B. Mitchell, Herschel K. Mitchell
In the presence of the gene, f, the growth rate of a poky strain becomes nearly normal but the defective cytochrome system remains unchanged, as if the defects were being compensated for by an increase in activity of some other enzyme system.
Proceedings of the National Academy of SciencesA Suppressor in Neurospora and its Use as Evidence for Allelism
45 Citations1947Mary B. Houlahan, Herschel K. Mitchell
An individual, homozygous for a suppressor gene in the mutant form and for the mutant gene upon which the suppressor acts, is phenotypically wild type, or nearly so.
ScienceCircadian Rhythms in <i>Neurospora crassa</i> : Oligomycin-Resistant Mutations Affect Periodicity
44 Citations1980Carol Diekmann, Stuart Brody
Nuclear mutations conferring resistance to oligomycin, a mitochondrial inhibitor, shorten the period of the circadian conidiation rhythm of Neurospora crassa from the normal 21.5 hours to 18 to 19 hours and slow the linear growth rate by 30 percent.
Carlsberg Research CommunicationsHuman meiosis III. Electron microscopical analysis of chromosome pairing in an individual with a balanced translocation 46, XY, t(5p−;22p+)
44 Citations1978Preben Bach Holm, Søren W. Rasmussen
The meiotic behaviour of a balanced translocation involving the short arms of chromosomes 5 and 22 has been analyzed by reconstructions of lateral components and synaptonemal complexes at early and mid pachytene in human spermatocytes to conclude that the translocation is reciprocal with transfer of the telomere region from chromosome 22 to chromosome 5 and that of the latter to chromosome 22.
GeneticsNUCLEAR DISTRIBUTION IN CONIDIA OF NEUROSPORA HETEROKARYONS
43 Citations1955K.C. Atwood, Frank Mukai
A simple approximation for the nuclear ratio from plating data will be given, based on assumptions differing in some ways from those of the randomness hypothesis and suggested by the nature of the observed departure from randomness.
Journal of BacteriologyLocalization of the β-Glucosidases in <i>Neurospora crassa</i>
43 Citations1970Bruce M. Eberhart, Reta S. Beck
The beta-glucosidases of Neurospora crassa were studied with respect to their location in conidia and young mycelia and measurements of K(m) suggested a slightly lower affinity for substrate p-nitrophenyl-beta-d- glucopyranoside by the enzymes in intact cells compared to enzymes in extracts.
GeneticsCOMPLEMENTATION ANALYSIS OF LINKED CIRCADIAN CLOCK MUTANTS OF <i>NEUROSPORA CRASSA</i>
43 Citations1976Jerry F. Feldman, Marian Hoyle
A fourth mutant of Neurospora crassa, designated frq-4, has been isolated in which the period length of the circadian conidiation rhythm is shortened to 19.3 hours, suggesting that all four mutations are allelic.
Journal of General MicrobiologyProperties of a Basic Amino Acid Permease in Neurospora crassa
42 Citations1968W. B. Roess, A. G. DeBusk
The system for transporting arginine into Neurospora conidia is described and shown to be consistent with the permease concept and to be separate from that which transports the aromatic and branched-chain neutral amino acids.
Journal of Biological ChemistryHomocitrate Formation in Neurospora crassa
41 Citations1968Robert W. Hogg, Harry P. Broquist
Neurospora crassa lysine auxotrophs 33933 and STL-7 were found to accumulate homocitrate and malate plus citrate, respectively, and it is shown that, in Neurospora, the initial step ofLysine biosynthesis is via a homocItrate-condensing enzyme which is under effective end product control by lysines.
Mutation Research/Fundamental and Molecular Mechanisms of MutagenesisMutagenesis at the ad-3A and ad-3B loci in haploid UV-sensitive strains of Neurospora crassa
40 Citations1980F.J. de Serres, Hirokazu Inoue +1 more
7 different mutations that confer sensitivity to inactivation by ultraviolet light have been investigated for their effect on spontaneous mutation in haploid strains of Neurospora crassa, and strain uvs-3 is highly mutable spontaneously with marked variation from experiment to experiment.
Mutation Research/Environmental Mutagenesis and Related SubjectsUse of the spot, plate and suspension test systems for the detection of the mutagenicity of environmental agents and chemical carcinogens in Neurospora crassa
40 Citations1978Tong-man Ong
Studies described here show that spot, plate and suspension tests using testers N23 and N24 are satisfactory substitutes for the ad-3 forward-mutation system for screening the mutagenic activity of environmental agents and chemical carcinogens in N. crassa.
Journal of BacteriologyMutants Affecting Thymidine Metabolism in <i>Neurospora crassa</i>
40 Citations1969Larry G. Williams, Herschel K. Mitchell
Three mutants were isolated in which different steps are blocked in the pathway that converts the pyrimidine ring of thymidine to an RNA precursor, and three other classes of mutations are proposed to be affecting, respectively, regulation of theThymidine degradative pathway, transport of pyridine free bases, and transport ofpyrimidine nucleosides.
Journal of BacteriologyPyrimidine Synthesis in <i>Neurospora crassa:</i> Gene-Enzyme Relationships
40 Citations1969Dina F. Caroline
A new series of pyrimidine-requiring mutants of Neurospora has been isolated and all enzymes involved in p Skyrimidine biosynthesis are represented by at least one mutant, including a single isolate for a new locus, pyr-6.
Journal of Molecular BiologyFrameshift mutations affecting the N-terminal sequence of Neurospora NADP-specific glutamate dehydrogenase
39 Citations1980Mohamed A M Siddig, John Á. Kinsey +2 more
A series of ultraviolet light-induced revertants from the mutant am6, mapping at the left-hand end of the structural gene for NADP-specific glutamate dehydrogenase, have been shown to have amino acid substitutions in the N-terminal tryptic peptide, meaning that am6 is a frameshift mutant.
GeneticsA GENE THAT CAUSES NATURAL DEATH IN NEUROSPORA CRASSA
39 Citations1951Sheng Teng
The irreparable mutant described in this paper is characterized on the one hand by a normal growth on minimal medium in the young condition and on the other hand by an ever-decreasing growth potential under any condition tested thus far.
GeneticsA MEIOTIC UV-SENSITIVE MUTANT THAT CAUSES DELETION OF DUPLICATIONS IN NEUROSPORA
39 Citations1978Dorothy Newmeyer, Donna R. Galeazzi
The meiotic-3 (mei-3) mutant of Neurospora crassa has several effects: when homozygous, it almost completely blocks meiosis and ascospore formation, it is sensitive to UV, its growth is inhibited by histidine and it increases the instability of nontandem duplications.
Proceedings of the National Academy of SciencesGenetic evidence on the organization and action of the qa-1 gene product: a protein regulating the induction of three enzymes in quinate catabolism in Neurospora crassa.
38 Citations1975Mary E. Case, Norman H. Giles
Genetic and biochemical evidence for both complementing and temperature-sensitive qa-1 alleles indicates that the product of the qA-1+ gene is an oligomeric (multimeric) protein, and the striking similarities between the qa system in this lower eukaryote and certain prokaryote operon systems are discussed.
Canadian Journal of Genetics and CytologyA SEARCH FOR COMPLEXITY AT THE MATING-TYPE LOCUS OF<i>NEUROSPORA CRASSA</i>
38 Citations1973Dorothy Newmeyer, H. Branch Howe +1 more
Evidence for complexity at the mating-type locus of Neurospora crassa was sought by selecting recombinants between closely linked markers on either side, and results indicate that if subunits exist, they are not more than 0.002 units apart.
Developmental BiologyAnalysis of pattern formation in neurospora perithecial development using genetic mosaics
38 Citations1976Thomas E. Johnson
Genetic mosaics used to elucidate the development of structures in the sexual phase of Neurospora provide a basis for understanding mechanisms of pattern formation in a structure which has been rather intractable to traditional cytological analysis.
GeneticsTHE INSTABILITY OF NEUROSPORA DUPLICATION <i>Dp(IL⇒IR)H4250</i>, AND ITS GENETIC CONTROL
37 Citations1977Dorothy Newmeyer, Donna R. Galeazzi
A genetic analysis of the instability of Dp(IL-->IR)H4250 reports on a smaller group of fertile escapes that are unlike either parental sequence; at least one of these involves a chromosome break outside the duplicated region.
Journal of BacteriologyInduction and Repression of Nitrate Reductase in <i>Neurospora crassa</i>
37 Citations1978Anne H. Dantzig, Wayne K. Zurowski +2 more
Nitrogen metabolites (other than ammonium) appear to be responsible for the mediation of "ammonium repression" in the regulation of this enzyme.
GeneticsCORRIGENDA
37 Citations1955C Yanofsky, D M Bonner
Observations indicate that suppressor gene specificity can be used to detect differences at the locus controlling the formation of tryptophan synthetase, and additional mutants and suppressors have been obtained and examined.
Journal of General MicrobiologyGene Interactions: the Mode of Action of the Suppressor of Acetate-requiring Mutants of Neurospora crassa
36 Citations1954Bernard S. Strauss, Steffen Pierog
Certain single-gene acetate-requiring mutants of Neurospora crassa are deficient in their ability to oxidize pyruvate directly, but can decarboxylate pyruVate to acetaldehyde and can form ethanol.
Canadian Journal of Genetics and CytologyISOLATION AND GENETIC ANALYSIS OF MMS-SENSITIVE<i>MUS</i>MUTANTS OF<i>NEUROSPORA</i>
36 Citations1980Etta Käfer, Esther Perlmutter
Results indicate that mus-8 may be epistatic to uvs-2 which is deficient in excision repair, but not to mutants like Uvs-3 that appear to be deficient in error-prone repair.
Journal of General MicrobiologySpectrum of Forward Mutants in the pyr-3 Region of Neurospora
36 Citations1963José L. Reissig
Evidence is presented which suggests that pyr-N and pyr su-arg lleles affect ATC via different mechanisms, and this results in pyrimidine-dependence and suppression of arg respectively.
Biochimica et Biophysica Acta (BBA) - General SubjectsRegulation of amino acid assimilations in a strain of Neurospora crassa lacking basic amino acid transport activity
35 Citations1969W M Thwaites, Lakshmi Pendyala
The bat mutant retains a generalized amino acid transport system which seems to be regulated to a high degree by alternations in arginine synthesis.
Archives of MicrobiologySome properties of vacuoles isolated from Neurospora crassa slime variant
35 Citations1979Enrico Martinoia, Urs Heck +3 more
A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described.
GeneticsGENETIC CONTROL OF THE STRUCTURE AND ACTIVITY OF AN ENZYME AGGREGATE IN THE TRYPTOPHAN PATHWAY OF <i>NEUROSPORA CRASSA</i>
35 Citations1967J A DeMoss, Robert W. Jackson +1 more
To define the role of the two genetic loci in controlling the structure and activity of the enzyme aggregate, a detailed biochemical and genetic analysis of a series of tryp-l andtryp-2 mutants is carried out.
Archives of Biochemistry and BiophysicsMolecular transport
35 Citations1971L. Wolfinbarger, A. G. DeBusk
The kinetic properties of this residual activity indicate a capability for transport of either neutral or basic amino acids and further suggests that it is under separate genetic control.
Journal of Biological ChemistryAcetylglutamate kinase. A mitochondrial feedback-sensitive enzyme of arginine biosynthesis in Neurospora crassa.
34 Citations1980Edward C. Wolf, R L Weiss
The radioisotopic method used to assay acetylglutamate kinase of Neurospora crassa has been shown to detect two distinct enzymatically catalyzed reactions, and the metabolic problems posed by a mitochondrial location of a feedback-sensitive enzyme and the cytosolic location of its effector are discussed.
Canadian Journal of Genetics and CytologyA NEW MUTATION IN<i>NEUROSPORA</i>FOR SENSITIVITY TO ULTRAVIOLET
34 Citations1968David Stadler, David A. Smith
A failure of the dark repair system for UV damage in Neurospora is determined by a single gene (uvs-2) located far out on the right arm of linkage group IV.
Journal of BacteriologyMultiple Alterations in Metabolite Uptake in a Mutant of <i>Neurospora crassa</i>
34 Citations1967Michael S. Kappy, Robert L. Metzenberg
A temperature-conditional lethal mutant of Neurospora crassa, un-t (55701), was resistant to neutral amino acid analogues by virtue of a decreased ability to transport these analogues and their natural congeners across the cell membrane and the possibility that the mutant has a generalized membrane defect is discussed.
Archives of Biochemistry and BiophysicsRepair of multiple defects of a regulatory mutant of neurospora by high osmotic pressure and by reversion
34 Citations1968Robert L. Metzenberg
A mutant, eth-1 r, of Neurospora crassa is ethionine-resistant, temperature-conditional lethal, and nonrepressible by methionine for the synthesis of aryl sulfatase, and raising the osmolar concentration with glycerol repairs all of these defects.
GeneticsGENETIC STUDIES OF ad-8 MUTANTS IN NEUROSPORA CRASSA. I. GENETIC FINE STRUCTURE OF THE ad-8 LOCUS
34 Citations1962Tatsuo Ishikawa
The genetic fine structure of the adenine-8 (ad-8) locus in Neurospora crassa is described, a locus at which a large number of mutants of independent origin are available and the existence of numerous distinct mutational sites within the ad-8 locus is indicated.
Journal of BacteriologyMucopolysaccharide Which Regulates Growth in <i>Neurospora</i>
34 Citations1971José L. Reissig, James E. Glasgow
Cultures of a colonial strain display a phase of slow growth; the induction of this phase is traced to the production of MP by the mold.
Proceedings of the National Academy of SciencesThe In Vitro Synthesis of Pantothenic Acid by Pantothenicless and Wild Type Neurospora
34 Citations1949Robert P. Wagner
Mutation Research/Fundamental and Molecular Mechanisms of MutagenesisMutagen sensitivities and mutator effects of MMS-sensitive mutants in neurospora
33 Citations1981Etta Käfer
7 mus (mutagen-sensitive) mutants of Neurospora crassa, which are more sensitive to the toxic effects of MMS than wild-type, were investigated for cross-sensitivities to other mutagens and inhibitors and found that mutants in 3 of these 5 genes showed radiation-induced mutation frequencies similar to wild- type.
GeneticsEVIDENCE FOR NONSENSE MUTATIONS IN THE <i>AROM</i> GENE CLUSTER OF <i>NEUROSPORA CRASSA</i>
33 Citations1968Mary E. Case, Norman H. Giles
Evidence is presented in support of the interpretation of these pleiotropic polar mutants resulting from mutations to nonsense codons within the proximal structural gene (arom-2) of the arom gene cluster, which is assumed to be transcribed in a polarized fashion via a single polycistronic messenger RNA.
Australian Journal of Biological SciencesOccurrence in Wild Strains of Neurospora crassa of Genes Controlling Genetic Recombination
32 Citations1975DG Catcheside
This work proves that there are minor differences between the control (con) genes, near to am-1 and his-2, which recognize products of rec-3 genes, and first clear evidence, though indirect, that the binding sites for products ofRec genes are situated in the chromosome regions where recombination is modulated.
Archives of Biochemistry and BiophysicsVitamin B6 antagonists and growth of microorganisms. I. 4-Desoxypyridoxine
32 Citations1953Jesse C. Rabinowitz, Esmond E. Snell
It is shown that the same organism may be sensitive to the inhibitor when dependent upon an external supply of vitamin B 6 for growth, but insensitive when cultured under conditions that permit it to synthesize this vitamin.
Proceedings of the National Academy of SciencesTHE q LOCUS OF NEUROSPORA CRASSA
32 Citations1956Patricia Lawrence
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