Estimation of Molecular Size and Molecular Weights of Biological Compounds by Gel Filtration
Methods of biochemical analysisPublished 1 January 1970
Paige G. Andrew
Citations639
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Abstract
This chapter contains sections titled: Introduction Elution Volume and Distribution Coefficient Theories of Gel Filtration Principles of the Gel Filtration Method of Molecular size and Molecular Weight Estimation Gel Filtration Media Special Aspects of Column Technique Estimation of Molecular Size (Stokes' Radius) by Column Methods Estimation of Molecular Weights by Column Methods Estimation of Molecular Weights by Thin-Layer Gel Filtration
Keywords
ChemistryBiochemistry, Genetics and Molecular BiologyEngineering
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The gels are easily regenerated in the columns and may be used daily over a period of months without detectable deterioration, and the column capacity is very high compared to other similar column methods (chromatography and zone electrophoresis).
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332 Citations1967A. David Smith, Hans Winkler
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A method is described for preparation of spherical agarose or agar grains, to be used as bed material for chromatographic “sieving” of molecules and particles.
BiochemistryStudies of Chemically Reacting Systems on Sephadex. I. Chromatographic Demonstration of the Gilbert Theory<sup>*</sup>
206 Citations1963Donald J. Winzor, Harold A. Scheraga
Archives of Biochemistry and BiophysicsA relationship between the molecular weights of macromolecules and their elution volumes based on a model for Sephadex gel filtration
199 Citations1964Phil G. Squire
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197 Citations1965B.B.L. Agrawal, Irwin Goldstein
Analytical Biochemistry“Molecular-sieve” chromatography of proteins on columns of cross-linked polyacrylamide
190 Citations1962Stellan Hjertén, Rolf Mosbach
Columns packed with cross-linked polyacrylamide have been used for chromatographic separation of high molecular weight substances, especially proteins, and there is a positive correlation between the molecular size of a protein and its Rf value.
Journal of Chromatography AThe correlation between molecular weight and elution behaviour in the gel chromatography of proteins
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A mathematical treatment of the Gel chromatography process, based on these empirical equations and avoiding assumptions about the microstructure of the gel, strengthens the concept of the exclusion mechanism.
Journal of Biological ChemistryPurification by Means of Detergents and Properties of Cytochrome b5 from Liver Microsomes
185 Citations1968Akihiro Ito, R Sato
It was concluded that cytochrome b5 preparations hitherto purified are protease-resistant cores of the native hemoprotein.
Carbohydrate ResearchStudies on uronic acid materials
167 Citations1966D.M.W. Anderson, J. Fraser Stoddart
Separation ScienceMolecular-Sieve Chromatography of Proteins on Granulated Polyacrylamide Gels
167 Citations1966J. Fawcett, C. J. O. R. Morris
The results are discussed in terms of the gel structure and a method for estimating the average effective pore radius in polyacrylamide gels of different compositions is derived and compared with experimental findings.
BiochemistryDetermination of molecular weight of proteins and protein subunits in the presence of 6M guanidine hydrochloride
165 Citations1968Agnès Ullmann, Michel Goldberg +2 more
BiochemistrySome Physical and Chemical Properties of Concanavalin A, the Phytohemagglutinin of the Jack Bean<sup>*</sup>
162 Citations1967Mark O. J. Olson, Irvin E. Liener
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149 Citations1967John J. Cebra, Parker A. Small
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The relation between the molecular weights of proteins and their behaviour on Sephadex gels is discussed and a method for the estimation of protein molecular weights from the results of thin-layer chromatography is derived.
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The following structure is proposed for the carbohydrate sequence in the chondroitin 4-sulfate-protein linkage region of bovine nasal septum: O-β-d-galactopyranosyl-(1 → 4)-d-xylose, respectively.
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136 Citations1965A. A. Leach, P.C. O'Shea
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The inhibition by some metals, and the lack of inhibition by other metals or by p -chloromercuribenzoate, indicate that SH groups are not required for full enzyme activity, whereas an imidazole group may be required.
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The dissociation of normal human oxyhemoglobin has been studied by gel filtration under conditions of neutral pH and moderate ionic strength, with the use of both integral boundaries, formed between solution and solvent, and finite difference boundaries,Forms a simple dimer-tetramer reversible equilibrium and a monomer-dimer-trimer-tetic reversible equilibrium.
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90 Citations1961Serge N. Timasheff, Robert Townend
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89 Citations1967Mark O. J. Olson, Irvin E. Liener
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72 Citations1968Dennis Saunders, Robert L. Pecsok
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The elution volumes of ovalbumin, trypsin and bovine serum albumin from a column of Sephadex G-100 have been shown to be functions of concentration; no such dependence is observed with Dextran-500.
Biochemical and Biophysical Research CommunicationsDissociation of protein subunits by maleylation
70 Citations1968C.L. Sia, B.L. Horecker
NatureAdsorption of Proteins on ‘Sephadex’
69 Citations1962Alexander N. Glazer, Daniel Wellner
Since ‘Sephadex’ has been used extensively for the desalting of protein solutions and for the separation of proteins from materials of low molecular weight, it is important to note that in attempts to remove salts completely by using distilled water as eluant, this phenomenon may lead to complications.
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68 Citations1967Th. Hohn, Heinz Schaller
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66 Citations1967Donald L. DeVincenzi, Jerry L. Hedrick
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65 Citations1966A.F.S.A. Habeeb
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65 Citations1965Katherine Selby, CC Maitland
There is no evidence for interconversion of, or synergism between, these components, and the effect of a change in temperature has been explained in terms of changes in the degree of swelling of the gel particles.
Journal of Biological ChemistryIsolation and Characterization of Lipopolysaccharides Containing 6-O-Methyl-d-glucose from Mycobacterium Species
65 Citations1966Yuan Chaun Lee
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64 Citations1966D.M.W. Anderson, J. Fraser Stoddart
Biochemical JournalBiosynthesis of an insulin precursor by islet tissue of cod (<i>Gadus callarias</i>)
64 Citations1968P. T. Grant, K.B.M. Reid
The results suggest that the polypeptide is an insulin precursor whose formation is inhibited by puromycin and that the steps involved in the conversion of precursor into product are sensitive to O-ethyl O-p-nitrophenyl phenylpropylphosphonate.
Analytical BiochemistryGel filtration of proteins, with particular reference to the glycoprotein, luteinizing hormone
63 Citations1965Darrell N. Ward, Marilyn S. Arnott
Results are reported which show that luteinizing hormone at pH 1.3 undergoes a marked change in its physical dimensions as studied by comparison with the gel filtration behavior of a series of simple proteins and two other glycoproteins on Sephadex G-75 at pH1.3 and pH 7.5.
Journal of Chromatography APropriétés échangeuses d'ions du gel de dextrane (sephadex)
62 Citations1962François Miranda, Hervé Rochat +1 more
European Journal of BiochemistrySedimentation Coefficient and Molecular Weight of Beef Liver Glutamate Dehydrogenase at the Microgram and the Milligram Level
61 Citations1968H. Sund, Walther Burchard
The molecular weight and the sedimentation behaviour of beef liver glutamate dehydrogenase was investigated and it was found that the associated enzyme molecule is formed in a stepwise association-dissociation equilibrium and not in a closed equilibrium between monomers and the octamer without any intermediate steps.
Proceedings of the National Academy of SciencesACHIEVEMENT OF SEDIMENTATION EQUILIBRIUM
58 Citations1961P. E. Hexner, L. E. Radford +1 more
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57 Citations1968J.L. GUERIGUIAN, W.H. Pearlman
The testosterone-binding component was readily separated from corticosteroid-binding globulin by column chromatography of pooled pregnancy serum on microgranular diethylaminoethyl cellulose and is distinctly different from corto-binding Globulin in its chromatographic behavior and steroid-binding properties.
Elsevier eBooksChemical Composition and Molecular Parameters of Purified Plasma Proteins
57 Citations1960Robert A. Phelps, Frank W. Putnam
This chapter discusses the delineation of the purified plasma proteins, and the chemical composition and molecular parameters of plasma proteins.
Journal of Lipid ResearchSeparation and size determination of human serum lipoproteins by agarose gel filtration
57 Citations1967Simeón Margolis
The technique was employed to demonstrate aggregation of low density lipoprotein following partial delipidation, partial proteolysis, or mild heat denaturation.
Canadian Journal of ChemistryPREPARATION OF SYNTHETIC GELS FOR CHROMATOGRAPHY OF MACROMOLECULES
55 Citations1962Daniel Lea, A.H. Sehon
NatureLayer Technique in Gel Filtration for estimating Differences in the Degree of Dissociation of Closely Related Proteins
52 Citations1966G. A. GILBERT
The leading and trailing boundaries do not then interfere with each other, and the effective elution position of a constituent can be found by constructing an equivalent sharp boundary using Longsworth's procedure for homogeneous solutions.
Journal of Chromatography ACorrelation between chromatographi and diffusional behavior of substances in beds of pore controlled glass
49 Citations1968Wolfgang Haller
It is very unlikely that a volume exclusion of uniform and controlle pore size display elution spectra similary to those of gel columns, and it is also unlikely that volume exclusion governs the elution spectrum in steric chromatography on gels.
Canadian Journal of ChemistryTHE USE OF POLYACRYLAMIDE GELS FOR CHROMATOGRAPHY OF PROTEINS
49 Citations1965Kai Sun, A.H. Sehon
Biochemical JournalPurification of an acid α-glucosidase by dextran-gel filtration
48 Citations1967Ferdinando Auricchio, Carmelo B. Bruni
The behaviour of rat liver α-glucosidases on dextran gel (Sephadex G-100) columns was studied and a ‘retardation’ of an acid α- GLUCosidase activity was observed.
Zeitschrift für Naturforschung BDie Trennung von Nucleinsäurebausteinen mit Sephadex
48 Citations1963Th. Hohn, W. Pollmann
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47 Citations1968Jaap Visser, C. Veeger
Association—dissociation phenomena are involved in the reversible conversion of activity with oxidized lipoate into DCIP activity in lipoamide dehydrogenase holoenzyme.
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