Yeast RNA Polymerase II Transcription Reconstituted with Purified Proteins
MethodsPublished 1 July 1997Open access
Lawrence C. Myers, Kerstin K. Leuther, David Bushnell, Claes M. Gustafsson, Roger D. Kornberg
Citations39
SJR quartileQ1
SJR score1.00
SNIP0.97
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TL;DR
This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins.
Abstract
Protocols are presented for the preparation of a fully defined yeast RNA polymerase II transcription system, consisting of essentially pure TFIIB, -E, -F, and -H, TATA-binding protein, RNA polymerase II, and mediator of transcriptional regulation. This system, comprising 44 polypeptides, is able to initiate transcription at any of a dozen yeast and mammalian promoters thus far tested and responds to a variety of transcriptional activator proteins.
Keywords
Biochemistry, Genetics and Molecular Biology
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