Expression and localization of matrilysin, a matrix metalloproteinase, in human endometrium during the reproductive cycle
American Journal of Obstetrics and GynecologyPublished 1 January 1993
William H. Rodgers, Kevin G. steen, Lynn M. Matrisian, Marc Navre, Linda C. Giudice, Fred Gorstein
Citations221
SJR quartileQ1
SJR score2.77
SNIP2.75
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
The expression of the matrilysin gene is regulated in endometrium during the reproductive cycle, implying an important role for matriolysin inendometrial physiologic characteristics.
Abstract
The expression of the matrilysin gene is regulated in endometrium during the reproductive cycle, implying an important role for matrilysin in endometrial physiologic characteristics.
Keywords
Immunology and MicrobiologyMedicine
Nucleic Acids ResearchEfficient<i>in vitro</i>synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
6,447 Citations1984Douglas A. Melton, Paul A. Krieg +4 more
It is shown that single stranded RNA probes of a high specific activity are easy to prepare and can significantly increase the sensitivity of nucleic acid hybridization methods.
The FASEB JournalMatrix metalloproteinases and their inhibitors in connective tissue remodeling
3,296 Citations1991J. Frederick Woessner
Latency is overcome by physical, chemical, and enzymatic treatments that separate the cysteine residue from the zinc Expression of the metalloproteinases is switched on by a variety of agents acting through regulatory elements of the gene, particularly the AP‐1 binding site.
Fertility and SterilityDating the Endometrial Biopsy
2,864 Citations1950R. W. Noyes, Arthur T. Hertig +1 more
It is asserted that examination of the endometrium during the secretory phase yields more information about the time of ovulation degree of progestational change and normality of theendometrium than any other test used in sterility studies.
Developmental BiologyDetection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes
1,635 Citations1984Kathleen H. Cox, Donna V. DeLeon +2 more
Estimates from the observed signals indicate that a large fraction of target RNAs is both retained in sections and hybridized with probe at saturation, and coupled with measurements of nonspecific background binding of heterologous probes, these data indicate that the method has sufficient sensitivity to detect many moderately abundant mRNAs.
Trends in GeneticsMetalloproteinases and their inhibitors in matrix remodeling
1,621 Citations1990Lynn M. Matrisian
Experiments designed to improve the understanding of metalloproteinase regulation have also resulted in new insights into mechanisms by which growth factors and proto-oncogenes may regulate biological processes.
DNAA Method for Isolation of Intact, Translationally Active Ribonucleic Acid
1,468 Citations1983Guy Cathala, Jean François Savouret +5 more
A method for isolation of large, translationally active RNA species is presented and yields large mRNA precursors up to 10 kb and mRNA species which translate very well, however, small RNA species are recovered with a poor yield.
NatureA novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas
1,133 Citations1990Paul Basset, Jean‐Pierre Bellocq +8 more
The suggestion is that stromelysin-3 is one of the stroma-derived factors that have long been postulated to play an important part in progression of epithelial malignancies.
The Histochemical JournalAminoalkylsilane-treated glass slides as support forin situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions
381 Citations1986Martin Rentrop, Bernhard Knapp +2 more
In situ hybridization was possible after a short immersion of the glass slides in a 2% solution of 3-aminopropyltriethoxysilane in acetone and it was found that especially harsh prehybridization drastically reduces the morphological integrity of the sections, thus rendering a reliable assignment of the label difficult.
Proceedings of the National Academy of SciencesEpidermal growth factor replaces estrogen in the stimulation of female genital-tract growth and differentiation.
380 Citations1991Karen G. Nelson, Tetsu Takahashi +3 more
The in vivo studies presented here demonstrate that epidermal growth factor (EGF) is an important autocrine and/or paracrine mediator of estrogen-induced growth and differentiation in mouse uterus and vagina and that EGF may serve as an important mediators of estrogen action in vivo.
Biochemical JournalThe collagenase gene family in humans consists of at least four members
365 Citations1988D Müller, Béatrice Quantin +4 more
It is concluded that the collagenase gene family in humans consists of at least four members, and speculate that expression of these genes plays a role in cancer.
Molecular CarcinogenesisExpression and localization of the matrix metalloproteinase pump‐1 (MMP‐7) in human gastric and colon carcinomas
281 Citations1991Susan McDonnell, Marc Navre +2 more
Using in situ hybridization and affinity purified anti‐pump‐1 antibodies, the expression of pump‐1 mRNA and protein was localized to tumor cells and was not detected in stromal or lymphocytic cells, suggesting that the inappropriate expression of pumps by malignant cells may contribute to the neoplastic phenotype.
Journal of Biological ChemistryPurification and properties of a small latent matrix metalloproteinase of the rat uterus.
221 Citations1988J. Frederick Woessner, Carolyn J Taplin
Its properties clearly distinguish it from collagenase, gelatinase, and stromelysin (matrix metalloproteinase 3); it constitutes a further member of the family of extracellular matrix metalloendopeptidases.
BiochemistryPump-1 cDNA codes for a protein with characteristics similar to those of classical collagenase family members
210 Citations1989Béatrice Quantin, Gillian Murphy +1 more
Results show that, despite the absence of a hemopexin-like domain, pump-1 is a latent secreted metalloproteinase.
Molecular Reproduction and DevelopmentExpression of the insulin‐like and platelet‐derived growth factor genes in human uterine tissues
164 Citations1990Keith D. Boehm, Makoto Daimon +4 more
The increased IGF‐I RNA in late proliferative–stage human endometrium correlates with the known elevation of estradiol secretion by the ovary and the increased concentration of uterine est radiol receptors during this stage of the menstrual cycle, which is consistent with reports of increased IGF'S RNA in the rat uterus in response to administration ofEstradiol.
The Journal of Clinical Endocrinology & MetabolismRegulation of Insulin-Like Growth Factor-Binding Protein-1 Synthesis and Secretion by Progestin and Relaxin in Long Term Cultures of Human Endometrial Stromal Cells*
144 Citations1991Stephen C. Bell, J Jackson +3 more
Results were consistent with a primary role of progestin exposure, whether in vivo or in vitro, in converting endometrial stromal cells to cells potentially able to exhibit the high rates of IGFBP-1 production typical of the decidualized endometrium of pregnancy.
Molecular EndocrinologyIdentification by Hybridization Histochemistry of Human Endometrial Cells Expressing mRNAs Encoding a Uterine β-Lactoglobulin Homologue and Insulin-Like Growth Factor-Binding Protein-1
110 Citations1990Mervi Julkunen, Riitta Koistinen +3 more
It is shown that beta LG/PP14 mRNA is expressed in the endometrium in a cyclic manner, being hardly detectable in midcycle and most abundant during the late secretory phase.
The Journal of Clinical Endocrinology & MetabolismInsulin-Like Growth Factor Binding Proteins in Human Endometrium: Steroid-Dependent Messenger Ribonucleic Acid Expression and Protein Synthesis*
99 Citations1991Linda C. Giudice, Deborah A. Milkowski +3 more
Examination of expression of the messenger RNAs encoding IGFBP-2 and IGF BP-3 in human proliferative endometrium shows that their expression is differentially regulated in secretory compared to proliferative phase endometrial (different steroidogenic environments), and that the synthesis of both IGFBP/IGF/IGBP-3 proteins is regulated by steroid hormones.
American Journal of Obstetrics and GynecologyEpidermal growth factor receptor expression in normal and malignant endometrium
75 Citations1989Andrew Berchuck, Andrew P. Soisson +5 more
Epidermal growth factor receptor expression in fresh-frozen uterine tissues was studied with the use of monoclonal antibody 528 and did not correlate with histologic grade, depth of myometrial invasion, estrogen-progesterone receptor status, the presence of extrauterine metastases, or the development of recurrent disease.
Proceedings of the National Academy of SciencesIncreased platelet-derived growth factor A-chain expression in human uterine smooth muscle cells during the physiologic hypertrophy of pregnancy.
46 Citations1990A E Mendoza, Regina Munter-Young +2 more
Observations demonstrate PDGF polypeptide expression in situ and implicate PDGF in a normal physiologic process--uterine expansion during pregnancy.
Gynecologic OncologyRegulation of epidermal growth factor and insulin-like growth factor I receptors by estradiol and progesterone in normal and neoplastic endometrial cell cultures
36 Citations1990R. Kevin Reynolds, Francisco Talavera +3 more
The effects of steroid hormones upon growth factor receptors in human endometrium are illustrated, and involvement of growth factors in the regulation of normal and neoplastic endometrial growth is suggested.
The Journal of Clinical Endocrinology & MetabolismDecidual Transformation of Human Extrauterine Mesenchymal Cells Is Associated with the Appearance of Insulin-Like Growth Factor-Binding Protein-1
25 Citations1991Eeva‐Marja Rutanen, SEPPO PARTANEN +1 more
It is described, for the first time, that cells morphologically similar to uterine decidual cells found beneath the peritoneal mesothelium in inguinal hernia and in the cervical mucosa during normal pregnancy react with the monoclonal antibody for IGFBP-1 equally to endometrial deciduality.
American Journal of Obstetrics and GynecologyHemodynamic and fluid responses to furosemide infusion in the ovine fetus
13 Citations1993Thomas F. Kelly, Thomas R. Moore +1 more
Furosemide infusion caused a marked dose-dependent diuresis in the ovine fetus that appears to be partly mediated by increases in vascular pressure.
American Journal of Obstetrics and GynecologyEpithelial cells from normal human endometrium express a tumor-associated glycoprotein (TAG-72) epitope in vitro
11 Citations1990Kevin G. Osteen, Ted L. Anderson +3 more
In vitro expression of tumor-associated glycoprotein 72 epitope by normal endometrial epithelial cells in vitro is in marked contrast to the cyclic, heterogeneous expression observed in vivo, and differs from published in vitro observations of cancer cell lines that express this epitope.
