Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast
Current BiologyPublished 1 February 1999Open access
Antonio Casamayor, Pamela D. Torrance, Takayasu Kobayashi, Jeremy Thorner, Dario R. Alessi
Citations262
SJR quartileQ1
SJR score2.71
SNIP1.83
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Two previously uncharacterised genes of Saccharomyces cerevisiae are found to encode protein kinases with catalytic domains closely resembling those of human and Drosophila PDK1, and these two groups of yeast kinases constitute two tiers of a signalling cascade required for yeast cell growth.
Abstract
Pkh1 and Pkh2 function similarly to PDK1, and Ypk1 and Ykr2 to SGK. As in animal cells, these two groups of yeast kinases constitute two tiers of a signalling cascade required for yeast cell growth.
Keywords
Biochemistry, Genetics and Molecular Biology
NatureCleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
251,640 Citations1970Ulrich K. Laemmli
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Molecular Cloning: A Laboratory Manual 2
129,982 Citations1989Joseph Sambrook, Elisabeth Fritsch +1 more
The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.
GeneticsA system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
8,794 Citations1989R Sikorski, P. Hieter
A series of yeast shuttle vectors and host strains has been created to allow more efficient manipulation of DNA in Saccharomyces cerevisiae to perform most standard DNA manipulations in the same plasmid that is introduced into yeast.
NatureInhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B
5,242 Citations1995Darren A.E. Cross, Dario R. Alessi +3 more
It is shown that agents which prevent the activation of both MAPKAP kinase-1 and p70S6k by insulin in vivo do not block the phosphorylation and inhibition of GSK3, and it is demonstrated that PKB is the product of the proto-oncogene protein kinase B (PKB, also known as Akt/RAC).
Journal of Biological ChemistryThe Tumor Suppressor, PTEN/MMAC1, Dephosphorylates the Lipid Second Messenger, Phosphatidylinositol 3,4,5-Trisphosphate
3,089 Citations1998Tomohiko Maehama, Jack E. Dixon
It is demonstrated that overexpression of PTEN, a putative tumor suppressor, reduced insulin-induced PtdIns(3,4,5)P3 production in human 293 cells without effecting insulin- induced phosphoinositide 3-kinase activation.
The EMBO JournalMechanism of activation of protein kinase B by insulin and IGF‐1.
3,029 Citations1996Dario R. Alessi, Mirjana Andjelković +5 more
It is demonstrated that activation of PKBalpha by insulin or insulin‐like growth factor‐1 (IGF‐1) results from phosphorylation of both Thr308 and Ser473, that phosphorylate of both residues is critical to generate a high level of P KBalpha activity and that the phosphorylated of Thr308 in vivo is not dependent on phosphorylations of Ser473 or vice versa.
GeneNew yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
2,894 Citations1988R. Daniel Gietz, Akio Sugino
The production of new alleles of the LEU2, URA3 and TRP1 genes of Saccharomyces cerevisiae by in vitro mutagenesis is described and a unique series of yeast-Escherichia coli shuttle vectors derived from the plasmid pUC19 are constructed.
Current BiologyCharacterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα
2,830 Citations1997Dario R. Alessi, Stephen R. James +5 more
PubMedProtein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.
2,543 Citations1995Steven K. Hanks, Tony Hunter
GeneA ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli
2,508 Citations1987Charles S. Hoffman, Fred Winston
To release plasmid DNA for the transformation of Escherichia coli, cells are subjected to vortex mixing in the presence of acid-washed glass beads, Triton X-100, sodium dodecyl sulfate, phenol and chloroform.
Molecular and Cellular BiologyIsolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
2,435 Citations1985Gérard I. Evan, George K. Lewis +2 more
MycologiaGuide to Yeast Genetics and Molecular Biology
2,384 Citations1993C. P. Kurtzman, Christine Guthrie +1 more
This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast.
Molecular and General Genetics MGGA positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
2,376 Citations1984Jef D. Boeke, Francois La Croute +1 more
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection, based on the loss of orotidine-5′-phosphate decarboxylase activity, and seems applicable to a variety of eucaryotic and procaryotic cells.
The FASEB JournalThe eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification <sup>1</sup>
2,300 Citations1995Steven K. Hanks, Tony Hunter
The eukaryotic protein kinases make up a large superfamily of homologous proteins, and a classification scheme can be founded on a kinase domain phylogeny, which reveals families of enzymes that have related substrate specificities and modes of regulation.
Current Opinion in Cell BiologyMechanisms and consequences of activation of protein kinase B/Akt
1,306 Citations1998Julian Downward
Activated PKB/Akt provides a survival signal that protects cells from apoptosis induced by various stresses, and also mediates a number of metabolic effects of insulin.
ScienceDual Role of Phosphatidylinositol-3,4,5-trisphosphate in the Activation of Protein Kinase B
1,209 Citations1997David Stokoe, Leonard R. Stephens +7 more
Chromatographic separation of brain cytosol revealed a kinase activity that phosphorylated and activated PKB only in the presence of phosphatidylinositol-3, 4,5-trisphosphate [PtdIns(3,4,5)P3].
The Annual Review of Pharmacology and ToxicologyTHE ROLE OF RECEPTOR KINASES AND ARRESTINS IN G PROTEIN–COUPLED RECEPTOR REGULATION
1,114 Citations1998Jason G. Krupnick, Jeffrey Benovic
The role of GRKs and arrestins in regulating agonist-specific signaling and trafficking of GPRs is discussed and endocytosis is linked to this process.
ScienceProtein Kinase C Isotypes Controlled by Phosphoinositide 3-Kinase Through the Protein Kinase PDK1
1,108 Citations1998J. Ann Le Good, Wolfgang Ziegler +4 more
Phosphorylation sites in members of the protein kinase A, PKG, and PKC kinase subfamily are conserved and the PKB kinase PDK1 may be responsible for the phosphorylation of PKC isotypes.
ScienceProtein Kinase B Kinases That Mediate Phosphatidylinositol 3,4,5-Trisphosphate-Dependent Activation of Protein Kinase B
1,061 Citations1998Len Stephens, Karen E. Anderson +10 more
Four isoforms of these PKB kinases were purified from sheep brain and their heterologous expression augments receptor activation of PKB, which suggests they are the primary signal transducers that enable PtdIns(3,4,5)P3 or PTDIns- (3, 4)P2 to activate PKB and hence to control signaling pathways regulating cell survival, glucose uptake, and glycogen metabolism.
CellThe GAP-related domain of the neurofibromatosis type 1 gene product interacts with ras p21
1,002 Citations1990George A. Martin, David Viskoohil +10 more
Kinetic measurements suggest that NF1 may be a significant regulator of ras p21 activity, particularly at low rAS p21 concentrations.
NatureRole of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun
978 Citations1994Irma Sánchez, Rowland T. Hughes +6 more
A novel protein kinase, called SAPK/ERK kinase-1 (SEK1), which is structurally related to the MAP kinase kinases (MEKs) and is a potent activator of the SAPKs in vitro and in vivo is identified.
Nucleic Acids ResearchRegulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression
975 Citations1994Dominik Mumberg, Rolf Müller +1 more
A series of compact vectors is designed for the convenient cloning of genes and their regulated expression at different levels and in various genetic backgrounds to compare the expression rates from different regulatable promoters at low or high copy number.
ScienceA Retroviral Oncogene, <i>akt</i> , Encoding a Serine-Threonine Kinase Containing an SH2-Like Region
885 Citations1991Alfonso Bellacosa, Joseph R. Testa +2 more
Sequence analysis of v-akt and biochemical characterization of its product revealed that it codes for a protein kinase C-related serine-threonine kinase whose cellular homolog is expressed in most tissues, with the highest amount found in thymus, suggesting that Akt may form a functional link between tyrosine and serinesine phosphorylation pathways.
SciencePhosphorylation and Activation of p70 <sup>s6k</sup> by PDK1
835 Citations1998Nick Pullen, Patrick B. Dennis +5 more
A regulatory link between p70s6k and PKB was demonstrated, as PDK1 was found to selectively phosphorylate p70S6k at Thr229, whereas the catalytically inactivePDK1 blocked insulin-induced activation of p70 s6k.
Current Opinion in Genetics & DevelopmentMechanism of activation and function of protein kinase B
750 Citations1998Dario R. Alessi, Philip Cohen
The past year has seen significant advances in understanding of how protein kinase B is activated and of the central role it plays in insulin signalling and in mediating the protective effects of survival factors against apoptosis.
Current Biology3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase
743 Citations1997Dario R. Alessi, Mária Deák +10 more
Human PDK1 is homologous to the Drosophila protein kinase DSTPK61, which has been implicated in the regulation of sex differentiation, oogenesis and spermatogenesis and is likely to mediate the activation of PKB by insulin or growth factors.
FEBS LettersMolecular basis for the substrate specificity of protein kinase B; comparison with MAPKAP kinase‐1 and p70 S6 kinase
635 Citations1996Dario R. Alessi, F. Barry Caudwell +3 more
Current BiologyRegulation of protein kinase C ζ by PI 3-kinase and PDK-1
617 Citations1998Margaret M. Chou, Weimin Hou +7 more
The results have identified PDK-1 as the kinase that phosphorylates and activates PKC zeta in the PI 3-kinase signaling pathway, and shows that a membrane-targeted PKCZeta is constitutively active in the absence of agonist stimulation.
Current Biology3-Phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates and activates the p70 S6 kinase in vivo and in vitro
581 Citations1998Dario R. Alessi, Mark T. Kozlowski +3 more
PDK1 is one of the components of the signaling pathway recruited by Pl 3-kinase for the activation of p70 S6 kinase as well as of PKB, and serves as a multifunctional effector downstream of the Pl 3 -kinase.
Journal of Biological ChemistryThe primary structure of the Saccharomyces cerevisiae gene for alcohol dehydrogenase.
560 Citations1982Jeffrey L. Bennetzen, B. D. Hall
The amino acid sequence of the protein as determined from the nucleotide sequence disagrees with the published alcohol dehydrogenase isozyme I (ADH-I) sequence for 5 of the 347 amino acid residues.
Proceedings of the National Academy of SciencesMolecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
537 Citations1991Pamela F. Jones, Teresa Jakubowicz +3 more
DNA sequence analysis identified an open reading frame of 1440 base pairs encoding a protein of 480 amino acids that was supported by the synthesis of a Mr 58,000 protein in an in vitro translation system that used RNA transcribed from cloned cDNAs with SP6 RNA polymerase.
ScienceAkt Signaling--Linking Membrane Events to Life and Death Decisions
534 Citations1997Brian A. Hemmings
In his Perspective, Hemmings discusses two reports that implicate the oncogenic kinase Akt in IGF-1-mediated neuronal survival and show how lipids can regulate the activity of Akt.
Molecular and Cellular BiologyCharacterization of <i>sgk</i>, a Novel Member of the Serine/Threonine Protein Kinase Gene Family Which Is Transcriptionally Induced by Glucocorticoids and Serum
526 Citations1993Melanie K. Webster, Luis Goya +3 more
This is the first report of a presumed serine/threonine protein kinase that is highly regulated at the transcriptional level by glucocorticoid hormones and suggests a novel interplay between glucoc Corticoid receptor signalling and aprotein kinase of the second messenger family.
European Journal of BiochemistryMolecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families
488 Citations1991Paul J. Coffer, James R. Woodgett
Of several novel cDNA fragments encoding putative kinases thus isolated, one was further characterised and its gene has thus been termed pkb, with elevated histone H1 kinase activity compared with similar fractions from control cells, demonstrating the enzymatic activity of this protein kinase.
NatureOsmotic stress activates phosphatidylinositol-3,5-bisphosphate synthesis
469 Citations1997Stephen K. Dove, Frank T. Cooke +4 more
It is shown that when the yeasts Saccharomyces cerevisiae and SchizosacCharomyces pombe are hyperosmotically stressed, they rapidly synthesize phosphatidylinositol-3,5-bisphosphate (PtdIns( 3,5)P2) by a process that involves activation of a PtdIns3P 5-OH kinase.
YeastA rapid method for localized mutagenesis of yeast genes
453 Citations1992Denise Muhlrad, Rosalyn Hunter +1 more
This procedure is efficient, allows trageting of specific regions for mutagenesis, and requires no subcloning steps in Escherichia coli.
Journal of Biological ChemistryHigh Affinity Binding of Inositol Phosphates and Phosphoinositides to the Pleckstrin Homology Domain of RAC/Protein Kinase B and Their Influence on Kinase Activity
444 Citations1997Matthias Frech, Mirjana Andjelković +4 more
Results demonstrate that the PH domain of RAC/PKB binds inositol phosphates and phosphoinositides with high affinity, and suggest that the products of the phosphatidylinositide 3-kinase can act as both a membrane anchor and modulator of Rac/P KB activity.
The EMBO JournalThe principal target of rapamycin‐induced p70s6k inactivation is a novel phosphorylation site within a conserved hydrophobic domain.
443 Citations1995Richard B. Pearson, Patrick B. Dennis +5 more
A combination of techniques are employed, including mass spectrometry, to demonstrate that the immunosuppressive agent rapamycin induces inactivation of p70s6k with no effect on other mitogen‐activated kinases, and that this effect is associated with selective dephosphorylation of three previously unidentified p70S6k phosphorylation sites.
Molecular and Cellular BiologyCharacterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum.
425 Citations1993M K Webster, L Goya +3 more
CellA candidate protein kinase C gene, PKC1, is required for the S. cerevisiae cell cycle
417 Citations1990David E. Levin, F.Owen Fields +3 more
Journal of Biological ChemistryRegulation of the p70 S6 Kinase by Phosphorylation in Vivo
383 Citations1998Qing-Ping Weng, Mark T. Kozlowski +4 more
Although p70 activity depends on a disinhibition from the carboxyl-terminal tail and the simultaneous phosphorylation at both Thr-252 and Thr-412, p70Activity in vivo is most closely related to the state of phosphorylated Thr- 412.
Biochemical JournalRole of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1
375 Citations1999A Currie, Kay S. WALKER +7 more
Results indicate that PtdIns(3,4,5)P3 plays several roles in the PDK1-induced activation of P KBalpha, and binds to the PH domain of PKB, altering its conformation so that it can be activated by PDK 1.
Biochemical JournalSpecific binding of the Akt-1 protein kinase to phosphatidylinositol 3,4,5-trisphosphate without subsequent activation
332 Citations1996Stephen R. James, C. Peter Downes +4 more
The EMBO JournalGPR1 encodes a putative G protein‐coupled receptor that associates with the Gpa2p Gα subunit and functions in a Ras‐independent pathway
324 Citations1998Xue Yong, Montserrat Batlle +1 more
It is shown that this growth control also involves a signal mediated by the heterotrimeric G protein α subunit homolog encoded by GPA2, consistent with a model in which the GPR1/GPA2 pathway activates the Sch9p kinase to generate a response that acts in parallel with that generated by the Ras/cAMP pathway, resulting in the integration of nutrient signals.
NatureThe nucleotide sequence of Saccharomyces cerevisiae chromosome VII
298 Citations1997Hervé Tettelin, Maria Luigia Carbone +113 more
No correlation was found between G+C content and gene density along the chromosome, and their variations are random, so accurate verification procedures demonstrate that there are less than two errors per 10,000 base pairs in the published sequence.
Nucleic Acids ResearchPrecise gene fusion by PCR
289 Citations1989Jeff Yon, Mike Fried
This fast and simple method for the construction of fusion genes using the polymerase chain reaction (PCR) has proved useful in creating gene fusions and deletions, and additional changes can be introduced at the join by modifying the linking oligo.
GeneGene disruption with PCR products in Saccharomyces cerevisiae
287 Citations1995Michael Lorenz, R.Scott Muir +4 more
It is found that regions of homology as short as 38 to 50 bp suffice to mediate homologous recombination in yeast and should facilitate analysis of sequenced genes of unknown function, which will soon include the entire yeast genome.
Biochemical JournalActivation of protein kinase B β and γ isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B α
280 Citations1998Kay S. Walker, Mária Deák +4 more
PKBgamma which had been activated by PDK1 possessed a substrate specificity identical with that of PKBalpha and PKBbeta towards a range of peptides, and was the major isoform activated by insulin in rat L6 myotubes (a skeletal-muscle cell line).
Proceedings of the National Academy of SciencesPhosphorylation and activation of cAMP-dependent protein kinase by phosphoinositide-dependent protein kinase
253 Citations1998Xiaodong Cheng, Yuliang Ma +3 more
PDK1, or one of its homologs, is a likely candidate for the in vivo PKA kinase that phosphorylates Thr-197, and opens a new dimension in the thinking about this ubiquitous protein kinase and how it is regulated in the cell.
FEBS LettersPDK1, one of the missing links in insulin signal transduction?<sup>1</sup>
246 Citations1997Philip Cohen, Dario R. Alessi +1 more
The evidence is reviewed which indicates that the phosphorylation of other proteins by PKB and GSK3 is likely to mediate many of the intracellular actions of insulin.
Current BiologyMultiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2
223 Citations1996W. Michael Kavanaugh, David Pot +8 more
These findings strongly implicate the inositol polyphosphate 5-phosphatases in Shc- and Grb2-mediated signal transduction in response to B-cell activation.
Genes & DevelopmentSCH9, a gene of Saccharomyces cerevisiae that encodes a protein distinct from, but functionally and structurally related to, cAMP-dependent protein kinase catalytic subunits.
201 Citations1988Takashi Toda, Scott J. Cameron +2 more
Trends in Biochemical Sciencesp70 S6 kinase: an enigma with variations
201 Citations1996Christopher G. Proud
Although the cellular role of S6 phosphorylation is still not fully clear, studies on p70S6k and its activation have revealed the existence of a novel signalling pathway, clues to the mechanism of action of certain immunosuppressants and insights into the control of gene expression at the levels of transcription and translation.
Proceedings of the National Academy of SciencesStructural and functional analysis of pp70S6k.
126 Citations1995Lynn Cheatham, Mercè Monfar +2 more
Proceedings of the National Academy of SciencesDominant genetics using a yeast genomic library under the control of a strong inducible promoter.
97 Citations1992Sandra W. Ramer, Stephen J. Elledge +1 more
Construction of a genomic DNA expression library for S. cerevisiae that allows isolation based on dominant phenotypes of genes that might have been difficult or impossible to isolate from high-copy-number libraries is reported.
Biochemical JournalRole of phosphatidylinositol 3,4,5-trisphosphate in regulating the activity and localization of 3-phosphoinositide-dependent protein kinase-1
82 Citations1999A Currie, Kay S. WALKER +7 more
NatureThe nucleotide sequence of Saccharomyces cerevisiae chromosome IV
80 Citations1997C. Jacq, J. Alt-Mörbe +134 more
Analysis of the sequence of Saccharomyces cerevisiae chromosome V revealed a centromere, two telomeres and 271 open reading frames (ORFs) plus 13 tRNAs and four small nuclear RNAs.
The Journal of Cell BiologyA novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus.
80 Citations1994Bret M. Benton, Jiatao Zang +1 more
The gene (FPR3) encoding a novel type of peptidylpropyl-cis-trans- isomerase (PPIase) was isolated during a search for previously unidentified nuclear proteins in Saccharomyces cerevisiae and demonstrated that Fpr3 is located exclusively in the nucleolus.
Journal of Biological ChemistryAnalysis of inositol metabolites produced by Saccharomyces cerevisiae in response to glucose stimulation.
73 Citations1993Phillip T. Hawkins, Len Stephens +1 more
The results suggest that deacylation of phosphatidylinositol 4-phosphate and phosphatIDylinposol 4,5-bisph phosphate may be coordinated with cell cycle control in S. cerevisiae.
Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles)The nucleotide sequence of chromosome IV from Saccharomyces cerevisiae
68 Citations1997Claude Jacq, Juliane Alt‐Mörbe +24 more
Molecular and General Genetics MGGA pair of putative protein kinase genes (YPK1 and YPK2) is required for cell growth in Saccharomyces cerevisiae
67 Citations1993Ping Chen, Kyung S. Lee +1 more
Cells deleted for both YPK1 and YPK2 were defective in vegetative growth, indicating that the protein kinases predicted to be encoded by these genes are functionally overlapping and play an essential role in the proliferation of yeast cells.
Molecular and General Genetics MGGA Schizosaccharomyces pombe gene, ksg1, that shows structural homology to the human phosphoinositide-dependent protein kinase PDK1, is essential for growth, mating and sporulation
59 Citations1999Christian Niederberger, M. Ernst Schweingruber
It is proposed that ksg1 is involved in one or several phosphoinositide signalling processes that are responsible for control of the life cycle and is lethal for haploid cells.
NatureThe nucleotide sequence of Saccharomyces cerevisiae chromosome XV
46 Citations1997Bernard Dujon, Kaj Albermann +95 more
The sequence of chromosome XV has been determined from a set of partly overlapping cosmid clones derived from a unique yeast strain, and physically mapped at 3-kilobase resolution before sequencing, and confirms the high proportion of orphan genes and reveals a number of ancestral and successive duplications with other yeast chromosomes.
GeneticsGeneration of temperature-sensitive cbp1 strains of Saccharomyces cerevisiae by PCR mutagenesis and in vivo recombination: characteristics of the mutant strains imply that CBP1 is involved in stabilization and processing of cytochrome b pre-mRNA.
35 Citations1993Robin R. Staples, Carol L. Dieckmann
To examine the activity of CBP1, temperature-sensitive cbp1 mutant strains were generated by polymerase chain reaction (PCR) mutagenesis and in vivo recombination and it was found that mature mRNAs are undetectable while precursor RNAs are reduced only slightly at the nonpermissive temperature.
DNAIsolation of a Yeast Protein Kinase Gene by Screening with a Mammalian Protein Kinase cDNA
28 Citations1988Richard A. Maurer
The gene for a new Saccharomyces cerevisiae protein kinase was identified and isolated by hybridization to a cloned cDNA for the catalytic subunit of bovine cAMP-dependentprotein kinase.
GeneA novel yeast gene coding for a putative protein kinase
20 Citations1989Kubo Kyoko, Shigeo Ohno +3 more
The encoded protein YKR2, containing 677 amino acids, shows about 40% sequence identity in the kinase region to a family of serine/threonine-specific protein kinases from various species.
Biochemical Society TransactionsRegulation of the <i>Saccharomyces cerevisiae</i> Vps34p phosphatidylinositol 3-kinase
5 Citations1997Daryll B. DeWald, Andrew E. Wurmser +1 more