Calcium is an intracellular mediator of the climbing fiber in induction of cerebellar long-term depression.
Proceedings of the National Academy of SciencesPublished 1 May 1990Open access
Masaki Sakurai
Citations267
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
In cerebellar Purkinje cells, conjunctive stimulation of parallel fibers and the climbing fiber causes long-term depression of parallel fiber-Purkinje cell transmission, and it has been postulated that calcium is an intracellular mediator of the climbing Fiber to induce this synaptic modification.
Abstract
In cerebellar Purkinje cells, conjunctive stimulation of parallel fibers and the climbing fiber causes long-term depression of parallel fiber-Purkinje cell transmission. It has been postulated that calcium is an intracellular mediator of the climbing fiber to induce this synaptic modification. To directly test the hypothesis, a calcium-chelating agent, EGTA, was intracellularly injected into Purkinje cells. In these injected cells, conjunctive stimulation failed to induce depression. Instead, it caused potentiation similar to that observed after repetitive stimulation of parallel fibers alone.
Keywords
NeuroscienceBiochemistry, Genetics and Molecular Biology
The Journal of PhysiologyLong‐lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path
7,290 Citations1973T.V.P. Bliss, Terje Lømo
The after‐effects of repetitive stimulation of the perforant path fibres to the dentate area of the hippocampal formation have been examined with extracellular micro‐electrodes in rabbits anaesthetized with urethane.
The Journal of PhysiologyElectrophysiological properties of in vitro Purkinje cell dendrites in mammalian cerebellar slices.
1,764 Citations1980R. Llinás, Mutsuyuki Sugimori
Intradendritic recordings from Purkinje cells in vitro indicate that white matter stimulation produces large synaptic responses by the activation of the climbing fibre afferent, but antidromic potentials do not actively invade the dendritic tree.
ScienceThe Neurobiology of Learning and Memory
1,163 Citations1986Richard F. Thompson
Probably applications of this new understanding of the neural bases of learning and memory range from education to the treatment of learning disabilities to the design of new artificial intelligence systems.
NatureIntracellular injections of EGTA block induction of hippocampal long-term potentiation
1,109 Citations1983Gary Lynch, John Larson +3 more
It is reported that intracellular injections of the calcium chelator EGTA block the development of LTP and this results strongly suggest that LTP is caused by a modification of the postsynaptic neurone and that its induction depends on the level of free calcium.
The Journal of PhysiologyClimbing fibre induced depression of both mossy fibre responsiveness and glutamate sensitivity of cerebellar Purkinje cells
966 Citations1982Masao Ito, Masaki Sakurai +1 more
In high decerebrate rabbits, cells were sampled extracellularly from the rostral flocculus and basket cells were identified based on the absence of olivary responses and also on their location in the molecular layer adjacent to identified Purkinje cells.
Progress in Biophysics and Molecular BiologyInactivation of Ca channels
610 Citations1984Roger Eckert, John E. Chad
Ca-dependent inactivation develops non-linearly with Ca entry and accumulation with CaEntry and accumulation, and Voltage-dependent activation may counteract Ca- dependent inactivation.
NatureIntracellular Ca2+ activates a fast voltage-sensitive K+ current in vertebrate sympathetic neurones
416 Citations1982Paul R. Adams, Andrew Constanti +2 more
It is deduced that in bullfrog sympathetic neurones IC is a fast current which can contribute a substantial fraction to the repolarizing current during an action potential, and is highly sensitive to external tetraethylammonium.
The Journal of PhysiologySynaptic modification of parallel fibre‐Purkinje cell transmission in in vitro guinea‐pig cerebellar slices.
396 Citations1987Masaki Sakurai
In in vitro slices of the cerebellum obtained from guinea‐pigs, synaptic transmission from parallel fibres to Purkinje cells and its modification by paired stimulation of parallel fibre and climbing fibres were studied, finding no associated changes in the membrane potential, the input resistance, or the magnitudes of climbing fibre responses in any of the cases.
The Journal of PhysiologyPossible mechanisms for long‐lasting potentiation of synaptic transmission in hippocampal slices from guinea‐pigs.
366 Citations1980P. Andersen, S. H. Sundberg +3 more
It is concluded that long‐lasting potentiation is specific to the pathway which has received the tetanization, and that the short‐lasting depression is not confined to any particular pathway but is a generalized (unspecific) phenomenon.
Brain ResearchLong-term depression of parallel fibre synapses following stimulation of climbing fibres
293 Citations1985C.‐F. Ekerot, Masanobu Kano
The results show that a short time of conjunctive stimulation of climbing fibres and parallel fibres results in a long-lasting depression of the parallel fibre responses and that this depression can be prevented if the Purkinje cells are inhibited during the conj unctive stimulation.
NatureQuisqualate receptors are specifically involved in cerebellar synaptic plasticity
288 Citations1987Masanobu Kano, Makoto Kato
This study compared the potency of several Glu agonists in inducing LTD and found a highly selective dependency of LTD on the quisqualate(QA)-selective subtype of Glu receptors.
NatureA Ca-dependent Cl− conductance in cultured mouse spinal neurones
165 Citations1984David G. Owen, Menahem Segal +1 more
It is reported here that many cultured mouse spinal neurones exhibit a voltage-activated chloride conductance that lasts for seconds and is dependent on extracellular calcium, thus complementing the functional roles of Ca-dependent K+ conductances.
The Journal of PhysiologyProlonged depolarization elicited in Purkinje cell dendrites by climbing fibre impulses in the cat.
164 Citations1981C.‐F. Ekerot, O. Oscarsson
Responses evoked in Purkinje cell dendrites by impulses in climbing fibres were studied by recording from the cat cerebellar cortex and the nature and significance of the plateau‐like depolarizations evoked by climbing fibre impulses in the purkinje cells are discussed.
The Journal of PhysiologyInteraction between responses in Purkinje cells evoked by climbing fibre impulses and parallel fibre volleys in the cat.
95 Citations1983N. C. Campbell, C.‐F. Ekerot +1 more
The results of the present investigation suggest that single dendritic branches of Purkinje cells serve as independent integrators of mossy fibre and climbing fibre inputs.
Brain ResearchVoltage clamp analysis of the effect of excitatory amino acids and derivatives on Purkinje cell dendrites in rat cerebellar slices maintained in vitro
62 Citations1983F. Crépel, Jean‐Luc Dupont +1 more
The results suggest that receptors of Purkinje cells for glutamate and aspartate are different, and are also different from N-methyl-D-aspartate and quisqualate receptors.
NeuroscienceFluctuation of extracellular potassium and calcium in the cerebellar cortex related to climbing fiber activity
39 Citations1980H. Sto ̈ckle, G. ten Bruggencate
Extracellular recordings performed with ion-selective microelectrodes showed that the amplitude fluctuations of climbing fiber-related field potentials were accompanied by corresponding changes in ion-activity levels, which could have functional implications for cerebellar information processing.