Expression of metabotropic glutamate receptors in murine thymocytes and thymic stromal cells
Journal of NeuroimmunologyPublished 1 September 2000
Marianna Storto, Ugo de Grazia, Giuseppe Battaglia, María Pía Felli, Marella Maroder, Alberto Gulino
Citations82
SJR quartileQ2
SJR score0.90
SNIP0.71
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TL;DR
MGlu receptors expressed in both thymic cell components were functional, as indicated by measurements of polyphosphoinositide hydrolysis or cAMP formation, and suggest a possible role for mGlu receptor signalling in the thymus.
Abstract
RT-PCR combined with immunoblotting showed the expression of group-I (mGlu1 and 5) and group-II (mGlu2 and 3) metabotropic glutamate receptors in whole mouse thymus, isolated thymocytes and TC-1S thymic stromal cell line. Cytofluorimetric analysis showed that mGlu-5 receptors were absent in CD4(-)/CD8(-) but present in more mature CD4(+) CD8(+) and CD4(+)CD8(-) thymocytes. mGlu-1a receptors showed an opposite pattern of expression with respect to mGlu5, whereas mGlu2/3 receptor expression did not differ between double negative and double positive cells. mGlu receptors expressed in both thymic cell components were functional, as indicated by measurements of polyphosphoinositide hydrolysis or cAMP formation. These data suggest a possible role for mGlu receptor signalling in the thymus.
Keywords
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