An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c.
Clinical ChemistryPublished 1 November 1984
Graham Ellis, Eleftherios P. Diamandis, Ernesto Giesbrecht, Denis Daneman, Lynn C. Allen
Citations80
SJR quartileQ1
SJR score1.34
SNIP1.39
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TL;DR
An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described, which incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration.
Abstract
An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.
Keywords
Biochemistry, Genetics and Molecular Biology
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