Partial amino acid sequence of rat pre-prolactin
Biochemical JournalPublished 1 January 1977Open access
R A Maurer, J Górski, D J McKean
Citations49
SJR quartileQ1
SJR score2.06
SNIP0.98
Generate an AI Snapshot to get a quick, structured summary of this paper.
Study Snapshot
ObjectiveStudy objective
MethodsResearch methodology
PopulationPopulation studied
Sample sizeSample sizes
OutcomesStudy outcomes here
ResultsStudy results comes here
LimitationsResearch study limitations comes here
A concise AI-generated summary of the paper will appear here once you click Generate AI Snapshot.
TL;DR
Rat pituitary mRNA was used to direct the cell-free synthesis of pre-prolactin labelled with [4,5-3H]leucine and either [35S] methioninc or [ 35S] cystine, and this partial sequence shows considerable similarity with other precursors that have been sequenced.
Abstract
Rat pituitary mRNA was used to direct the cell-free synthesis of pre-prolactin labelled with [4,5-3H]leucine and either [35S] methioninc or [35S] cystine. Sequence analysis of the labelled protein indicates that pre-prolactin has 29 amino acid residues joined to the N-terminus of the prolactin sequence. Leucine residues were found at positions 13, 14, 15, 16, 21 and 22, methionine residues at positions 1, 17 and 18, and a cysteine residue at position 24 of the precursor sequence, and this partial sequence shows considerable similarity with other precursors that have been sequenced.
Keywords
MedicineBiochemistry, Genetics and Molecular Biology
The Journal of Cell BiologyTransfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
3,011 Citations1975Günter Blobel, B Dobberstein
These results established that processing in vivo takes place before completion of the nascent chain, and indicate that in vitro processing of nascent chains by rough microsomes is dependent on ribosome binding to the membrane.
BiochemistryQuantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac
679 Citations1971O. Smithies, D. M. Gibson +4 more
Proceedings of the National Academy of SciencesCell-free synthesis of rat preproinsulins: characterization and partial amino acid sequence determination.
281 Citations1976Shu Jin Chan, P S Keim +1 more
This peptide preproinsulin is designated on the basis of evidence that whole nucleic acid fractions of isolated rat islets of Langerhans greatly stimulate incorporation of radioactive amino acids into protein in a wheat germ ribosomal system.
Proceedings of the National Academy of SciencesHomology in amino-terminal sequence of precursors to pancreatic secretory proteins.
273 Citations1975Anne Devillers‐Thiéry, Thomas J. Kindt +2 more
It is suggested that this common sequence constitutes a metabolically short-lived peptide extension which precedes the amino-terminal sequences of all pancreatic secretory proteins and that it functions in the transfer of these proteins across the microsomal membrane.
Proceedings of the National Academy of SciencesPurification and Properties of Biologically Active Messenger RNA for a Myeloma Light Chain
195 Citations1972Daniel Swan, Haim Aviv +1 more
The results suggest that the light-chain mRNA is monocistronic and that it contains about 200 more bases than would be necessary to encode the variable and constant regions of a single light- chain molecule.
Proceedings of the National Academy of SciencesPre-proparathyroid Hormone: A Direct Translation Product of Parathyroid Messenger RNA
193 Citations1974Byron Kemper, Joel F. Habener +3 more
A protein corresponding to the cell-free product could not be detected in intact cells even during incubations with [(3)H]leucine as short as 2 min, which suggests the protein may be a transient precursor to proparathyroid hormone.
Journal of Biological ChemistryPurification and Properties of an Enzyme from Beef Liver Which Catalyzes Sulfhydryl-Disulfide Interchange in Proteins
139 Citations1966Francesco De Lorenzo, Robert F. Goldberger +3 more
An enzyme which catalyzes sulfhydryl-disulfide interchange in proteins containing "incorrect" disulfide bonds has been isolated from beef liver microsomes and shows two bands upon electrophoresis in starch gels and polyacrylamide gels.
Proceedings of the National Academy of SciencesmRNA-dependent synthesis of authentic precursor to human placental lactogen: conversion to its mature hormone form in ascites cell-free extracts.
122 Citations1976Elżbieta Szczȩsna, Irving Boime
Data strongly suggest that pre-hPL is an authentic percursor to hPL, cleavage of the precursor primarily occurs on nascent, ribosome-bound peptide chains, and pre- hPL is the primary gene product.
BiochemistryPre-proparathyroid hormone: Analysis of radioactive tryptic peptides and amino acid sequence
120 Citations1976Byron Kemper, Joel F. Habener +3 more
The amino acid sequence of bovine pre-proparathyroid hormone has been partially determined by analysis of the polypeptide labeled selectively with radioactive amino acids, and the identities of 17 of the 25 amino acids have been established.
Proceedings of the National Academy of SciencesPregrowth hormone: product of the translation in vitro of messenger RNA coding for growth hormone.
114 Citations1976P M Sussman, Robert Tushinski +1 more
The results strongly imply that the cell-free product represents a growth hormone precursor (pregrowth hormone) which is rapidly converted to growth hormone in pituitary cells.
Journal of Biological ChemistryCell-free synthesis of a large translation product of prolactin messenger RNA.
111 Citations1976R A Maurer, R. T. Stone +1 more
The results of these studies suggest that prolactin messenger RNA directs the cell-free synthesis of a product which contains the amino acid sequence of Prolactin but which has an addition at one or both ends of the molecule.
Journal of Biological ChemistryCell-free synthesis of a prolactin precursor directed by mRNA from cultured rat pituitary cells.
88 Citations1976Gerald A. Evans, M G Rosenfeld
Levels of mRNA coding for prolactin determined by translation of total mRNA and quantitation of preprolactin synthesized were stimulated 3- to 6-fold relative to the levels in control cultures, suggesting that regulation of prolactIn synthesis was accomplished, at least in part, at a transcriptional level.
Biochemical and Biophysical Research CommunicationsThyrotropin-releasing hormone inreases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free system
82 Citations1976Priscilla S. Dannies, Armen H. Tashijian
Biochemical and Biophysical Research CommunicationsThe translation of a human placental lactogen mRNA fraction in heterologous cell-free systems: The synthesis of a possible precursor
78 Citations1975Irving Boime, Sophie Boguslawski +1 more
RNA extracted from term placental homogenates directed the synthesis of human placental lactogen (HPL) in a heterologous cell-free system derived from Krebs II ascites tumor cells, raising the possibility that a HPL precursor was synthesized in the wheat germ system.
European Journal of BiochemistryTranslation of the mRNA for Rabbit Uteroglobin in Cell‐Free Systems
54 Citations1976Miguel Beato, Antonio Nieto
The endometrium of intact animals treated with estradiol alone also contains the same mRNA bound to polysomes but in a smaller proportion, indicating that the progesterone-induced synthesis of uteroglobin is accompanied by an accumulation of the specific mRNA in the polysome.
European Journal of BiochemistryTranslation of Honeybee Promelittin Messenger RNA
37 Citations1975Gerda Suchanek, Ingela Kindås-Mügge +2 more
Evidence is presented that most of the extra amino acids are located at the amino terminus of the product formed in vitro and the larger polypeptide detected in vitro may represent a precursor of promelittin.
BiochemistryAmino acid sequence determination with radioactive proteins
36 Citations1974D J McKean, Erwin H. Peters +2 more
Biochemical JournalAmino acid-sequence variability at the <i>N</i>-terminal extra piece of mouse immunoglobulin light-chain precursors of the same and different subgroups
34 Citations1976Yigal Burstein, Israël Schechter
The proteins programmed in the wheat-germ cell-free system by the mRNA coding for the MOPC-63 mouse myeloma L (light) chain were labelled with six radioactive amino acids, which suggest that the extra piece is part of the variable region of the L chain.
BiochemistryTranslation of satellite tobacco necrosis virus ribonucleic acid. II. Initiation of in vitro translation in procaryotic and eucaryotic systems
25 Citations1972R Lundquist, Jerome M. Lazar +2 more
The theory that the original, eucaryotic, STNV-RNA translation product has specifically lost an N-terminal methionine to yield an alanine-terminated protein is supported.