Denervation supersensitivity in skeletal muscle: analysis with a cloned cDNA probe.
The Journal of Cell BiologyPublished 1 July 1984Open access
John P. Merlie, K E Isenberg, Steven D. Russell, Joshua R. Sanes
Citations192
SJR quartileQ1
SJR score3.36
SNIP1.52
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TL;DR
Hybridization analysis with a cloned cDNA specific for AChR alpha-subunit was used to compare the abundance of A ChR mRNA in innervated and denervated adult mouse muscles, and the increase in AchR mRNA level was sufficiently large and rapid to account for denervation supersensitivity.
Abstract
Motor neurons regulate the acetylcholine sensitivity of the muscles they innervate: denervated muscle fiber become "supersensitive" to acetylcholine, due to insertion of newly synthesized acetylcholine receptors (AChRs) in the plasma membrane. We used hybridization analysis with a cloned cDNA specific for AChR alpha-subunit to compare the abundance of AChR mRNA in innervated and denervated adult mouse muscles. Within 3 d of denervation, levels of AChR mRNA increased 100-fold; levels of actin mRNA changed little. The increase in AChR mRNA level was sufficiently large and rapid to account for denervation supersensitivity.
Keywords
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