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A comparison of five methods for assaying bacterial hydrophobicity

Journal of Microbiological MethodsPublished 1 October 1986
Jasmine K. Dillon, John A. Fuerst, Alan C. Hayward, G. H. G. Davis
Citations105
SJR quartileQ3
SJR score0.48
SNIP0.65

TL;DR

Five methods for assaying bacterial surface hydrophobicity, namely, bacterial adherence to hydrocarbons, salt aggregation, hydrophobic interaction chromatography, adhesion to polystyrene and latex particle agglutination were used, conclude that reliance on one method for such tests is inadequate.

Abstract

Five methods for assaying bacterial surface hydrophobicity, namely, bacterial adherence to hydrocarbons, salt aggregation, hydrophobic interaction chromatography, adhesion to polystyrene and latex particle agglutination were used to compare the hydrophobic surface properties of Escherichia coli, Acinetobacter calcoaceticus, Staphylococcus aureus and Streptococcus mitis. Two strains of A. calcoaceticus, including RAG-1, gave strong positive results by all five methods. S. mitis gave weak or negative results by all methods. The results for the other bacteria varied with the method. We conclude that reliance on one method for such tests is inadequate.

Keywords

Agricultural and Biological SciencesBiochemistry, Genetics and Molecular Biology